Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1875 and GFF1876 are separated by 470 nucleotides GFF1876 and GFF1877 are separated by 2 nucleotides GFF1877 and GFF1878 are separated by 18 nucleotides GFF1878 and GFF1879 are separated by 18 nucleotides
GFF1875 - Lysine--tRNA ligase, at 536,717 to 538,393
GFF1875
GFF1876 - Aminomethyltransferase, at 538,864 to 540,030
GFF1876
GFF1877 - Glycine cleavage system H protein, at 540,033 to 540,404
GFF1877
GFF1878 - hypothetical protein, at 540,423 to 540,899
GFF1878
GFF1879 - putative glycine dehydrogenase (decarboxylating) subunit 1, at 540,918 to 542,258
GFF1879
Position (kb) 538
539
540
541 Strain fitness (log2 ratio) -1
0
1 at 538.504 kb on + strand at 538.809 kb on + strand at 539.479 kb on + strand, within GFF1876 at 539.497 kb on + strand, within GFF1876 at 539.498 kb on - strand, within GFF1876 at 539.624 kb on - strand, within GFF1876 at 539.896 kb on + strand, within GFF1876 at 539.897 kb on - strand, within GFF1876 at 539.990 kb on + strand at 539.990 kb on + strand at 540.063 kb on + strand at 540.064 kb on - strand at 540.064 kb on - strand at 540.225 kb on + strand, within GFF1877 at 540.225 kb on + strand, within GFF1877 at 540.225 kb on + strand, within GFF1877 at 540.225 kb on + strand, within GFF1877 at 540.226 kb on - strand, within GFF1877 at 540.226 kb on - strand, within GFF1877 at 540.226 kb on - strand, within GFF1877 at 540.378 kb on + strand at 540.378 kb on + strand at 540.854 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 538,504 + +1.0 538,809 + +0.2 539,479 + GFF1876 0.53 -0.3 539,497 + GFF1876 0.54 -0.5 539,498 - GFF1876 0.54 -0.7 539,624 - GFF1876 0.65 +0.1 539,896 + GFF1876 0.88 -1.0 539,897 - GFF1876 0.89 -0.2 539,990 + +0.0 539,990 + +0.3 540,063 + +0.3 540,064 - -0.2 540,064 - -0.9 540,225 + GFF1877 0.52 -0.3 540,225 + GFF1877 0.52 -0.0 540,225 + GFF1877 0.52 +0.7 540,225 + GFF1877 0.52 +0.4 540,226 - GFF1877 0.52 +0.6 540,226 - GFF1877 0.52 -0.5 540,226 - GFF1877 0.52 +0.5 540,378 + -0.9 540,378 + -0.1 540,854 - +0.1
Or see this region's nucleotide sequence