Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1863 and GFF1864 are separated by 4 nucleotides GFF1864 and GFF1865 are separated by 91 nucleotides GFF1865 and GFF1866 are separated by 30 nucleotides GFF1866 and GFF1867 are separated by 413 nucleotides
GFF1863 - lipid kinase YegS, at 524,712 to 525,647
GFF1863
GFF1864 - hypothetical protein, at 525,652 to 526,440
GFF1864
GFF1865 - hypothetical protein, at 526,532 to 527,353
GFF1865
GFF1866 - hypothetical protein, at 527,384 to 527,773
GFF1866
GFF1867 - Replicative DNA helicase, at 528,187 to 529,668
GFF1867
Position (kb) 526
527
528 Strain fitness (log2 ratio) -1
0
1
2 at 525.547 kb on - strand, within GFF1863 at 525.720 kb on - strand at 525.912 kb on - strand, within GFF1864 at 526.055 kb on + strand, within GFF1864 at 526.056 kb on - strand, within GFF1864 at 526.287 kb on + strand, within GFF1864 at 526.287 kb on + strand, within GFF1864 at 526.287 kb on + strand, within GFF1864 at 526.289 kb on + strand, within GFF1864 at 526.290 kb on - strand, within GFF1864 at 526.409 kb on + strand at 526.409 kb on + strand at 526.409 kb on + strand at 526.409 kb on + strand at 526.409 kb on + strand at 526.409 kb on + strand at 526.409 kb on + strand at 526.410 kb on - strand at 526.410 kb on - strand at 526.410 kb on - strand at 526.542 kb on + strand at 526.542 kb on + strand at 526.722 kb on + strand, within GFF1865 at 526.723 kb on - strand, within GFF1865 at 526.827 kb on + strand, within GFF1865 at 526.828 kb on - strand, within GFF1865 at 526.828 kb on - strand, within GFF1865 at 526.830 kb on + strand, within GFF1865 at 527.218 kb on - strand, within GFF1865 at 527.218 kb on - strand, within GFF1865 at 527.218 kb on - strand, within GFF1865 at 527.301 kb on + strand at 527.302 kb on - strand at 527.422 kb on - strand at 527.511 kb on + strand, within GFF1866 at 527.511 kb on + strand, within GFF1866 at 527.511 kb on + strand, within GFF1866 at 527.511 kb on + strand, within GFF1866 at 527.512 kb on - strand, within GFF1866 at 527.616 kb on + strand, within GFF1866 at 527.616 kb on + strand, within GFF1866 at 527.616 kb on + strand, within GFF1866 at 527.616 kb on + strand, within GFF1866 at 527.616 kb on + strand, within GFF1866 at 527.616 kb on + strand, within GFF1866 at 527.617 kb on - strand, within GFF1866 at 527.617 kb on - strand, within GFF1866 at 527.617 kb on - strand, within GFF1866 at 527.859 kb on - strand at 527.882 kb on - strand at 527.910 kb on + strand at 528.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 525,547 - GFF1863 0.89 +0.2 525,720 - -1.1 525,912 - GFF1864 0.33 -1.6 526,055 + GFF1864 0.51 -0.9 526,056 - GFF1864 0.51 -0.6 526,287 + GFF1864 0.80 -0.5 526,287 + GFF1864 0.80 +0.6 526,287 + GFF1864 0.80 +1.0 526,289 + GFF1864 0.81 -0.1 526,290 - GFF1864 0.81 -0.8 526,409 + +0.7 526,409 + +0.2 526,409 + +0.3 526,409 + -1.2 526,409 + +0.5 526,409 + -0.5 526,409 + -0.3 526,410 - -0.8 526,410 - +0.2 526,410 - +0.1 526,542 + +1.0 526,542 + -0.1 526,722 + GFF1865 0.23 +0.1 526,723 - GFF1865 0.23 +1.6 526,827 + GFF1865 0.36 -0.3 526,828 - GFF1865 0.36 +2.0 526,828 - GFF1865 0.36 +0.7 526,830 + GFF1865 0.36 -0.9 527,218 - GFF1865 0.83 +1.6 527,218 - GFF1865 0.83 +1.9 527,218 - GFF1865 0.83 +1.4 527,301 + -0.3 527,302 - +0.4 527,422 - +1.6 527,511 + GFF1866 0.33 -0.5 527,511 + GFF1866 0.33 +0.0 527,511 + GFF1866 0.33 +0.1 527,511 + GFF1866 0.33 +0.2 527,512 - GFF1866 0.33 -0.3 527,616 + GFF1866 0.59 +0.3 527,616 + GFF1866 0.59 -0.3 527,616 + GFF1866 0.59 +0.2 527,616 + GFF1866 0.59 -0.1 527,616 + GFF1866 0.59 -0.3 527,616 + GFF1866 0.59 -1.1 527,617 - GFF1866 0.60 +0.2 527,617 - GFF1866 0.60 -0.5 527,617 - GFF1866 0.60 -0.5 527,859 - +1.7 527,882 - +2.0 527,910 + +0.5 528,067 - +0.4
Or see this region's nucleotide sequence