Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF176 and GFF177 are separated by 126 nucleotides GFF177 and GFF178 overlap by 1 nucleotides GFF178 and GFF179 overlap by 1 nucleotides
GFF176 - hypothetical protein, at 184,786 to 185,547
GFF176
GFF177 - Lipopolysaccharide export system ATP-binding protein LptB, at 185,674 to 186,528
GFF177
GFF178 - High-affinity branched-chain amino acid transport system permease protein LivH, at 186,528 to 187,406
GFF178
GFF179 - hypothetical protein, at 187,406 to 188,449
GFF179
Position (kb) 186
187
188 Strain fitness (log2 ratio) -1
0
1
2 at 185.872 kb on + strand, within GFF177 at 185.873 kb on - strand, within GFF177 at 185.873 kb on - strand, within GFF177 at 186.065 kb on - strand, within GFF177 at 186.179 kb on - strand, within GFF177 at 186.179 kb on - strand, within GFF177 at 186.369 kb on + strand, within GFF177 at 186.370 kb on - strand, within GFF177 at 186.370 kb on - strand, within GFF177 at 186.370 kb on - strand, within GFF177 at 186.464 kb on - strand at 186.464 kb on - strand at 186.464 kb on - strand at 186.464 kb on - strand at 186.464 kb on - strand at 186.619 kb on - strand, within GFF178 at 186.658 kb on + strand, within GFF178 at 186.658 kb on + strand, within GFF178 at 186.659 kb on - strand, within GFF178 at 186.939 kb on + strand, within GFF178 at 186.939 kb on + strand, within GFF178 at 186.939 kb on + strand, within GFF178 at 186.939 kb on + strand, within GFF178 at 186.939 kb on + strand, within GFF178 at 186.940 kb on - strand, within GFF178 at 186.940 kb on - strand, within GFF178 at 186.940 kb on - strand, within GFF178 at 186.940 kb on - strand, within GFF178 at 186.940 kb on - strand, within GFF178 at 187.068 kb on + strand, within GFF178 at 187.069 kb on - strand, within GFF178 at 187.101 kb on + strand, within GFF178 at 187.314 kb on + strand, within GFF178 at 187.315 kb on - strand, within GFF178 at 187.315 kb on - strand, within GFF178 at 187.335 kb on + strand at 187.433 kb on + strand at 187.433 kb on + strand at 187.542 kb on - strand, within GFF179 at 187.542 kb on - strand, within GFF179 at 187.605 kb on - strand, within GFF179 at 187.605 kb on - strand, within GFF179 at 187.606 kb on + strand, within GFF179 at 187.607 kb on - strand, within GFF179 at 187.656 kb on - strand, within GFF179 at 187.657 kb on + strand, within GFF179 at 187.658 kb on - strand, within GFF179 at 187.658 kb on - strand, within GFF179 at 187.658 kb on - strand, within GFF179 at 187.705 kb on + strand, within GFF179 at 187.705 kb on + strand, within GFF179 at 187.706 kb on - strand, within GFF179 at 187.743 kb on + strand, within GFF179 at 187.743 kb on + strand, within GFF179 at 187.744 kb on - strand, within GFF179 at 187.744 kb on - strand, within GFF179 at 187.744 kb on - strand, within GFF179 at 187.744 kb on - strand, within GFF179 at 187.781 kb on + strand, within GFF179 at 187.782 kb on - strand, within GFF179 at 187.926 kb on - strand, within GFF179 at 188.027 kb on - strand, within GFF179 at 188.063 kb on + strand, within GFF179 at 188.127 kb on - strand, within GFF179 at 188.127 kb on - strand, within GFF179 at 188.178 kb on - strand, within GFF179 at 188.274 kb on - strand, within GFF179 at 188.274 kb on - strand, within GFF179 at 188.373 kb on + strand at 188.374 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 185,872 + GFF177 0.23 +0.6 185,873 - GFF177 0.23 -0.0 185,873 - GFF177 0.23 -0.3 186,065 - GFF177 0.46 -1.0 186,179 - GFF177 0.59 +0.2 186,179 - GFF177 0.59 -0.6 186,369 + GFF177 0.81 -0.1 186,370 - GFF177 0.81 -0.5 186,370 - GFF177 0.81 -1.2 186,370 - GFF177 0.81 -1.7 186,464 - -0.3 186,464 - -0.7 186,464 - +0.5 186,464 - -0.1 186,464 - +0.9 186,619 - GFF178 0.10 +0.6 186,658 + GFF178 0.15 -0.7 186,658 + GFF178 0.15 -0.1 186,659 - GFF178 0.15 -0.4 186,939 + GFF178 0.47 +0.1 186,939 + GFF178 0.47 -0.3 186,939 + GFF178 0.47 +0.0 186,939 + GFF178 0.47 +0.1 186,939 + GFF178 0.47 -0.1 186,940 - GFF178 0.47 -0.8 186,940 - GFF178 0.47 +0.1 186,940 - GFF178 0.47 -0.7 186,940 - GFF178 0.47 -0.6 186,940 - GFF178 0.47 -0.0 187,068 + GFF178 0.61 -0.5 187,069 - GFF178 0.62 -0.9 187,101 + GFF178 0.65 +0.5 187,314 + GFF178 0.89 -0.5 187,315 - GFF178 0.90 -0.3 187,315 - GFF178 0.90 +0.6 187,335 + +0.3 187,433 + -0.4 187,433 + +0.0 187,542 - GFF179 0.13 -0.3 187,542 - GFF179 0.13 -0.3 187,605 - GFF179 0.19 -0.2 187,605 - GFF179 0.19 +0.7 187,606 + GFF179 0.19 -0.2 187,607 - GFF179 0.19 +0.4 187,656 - GFF179 0.24 -0.2 187,657 + GFF179 0.24 +0.1 187,658 - GFF179 0.24 -1.4 187,658 - GFF179 0.24 +1.4 187,658 - GFF179 0.24 -0.9 187,705 + GFF179 0.29 -0.4 187,705 + GFF179 0.29 -0.4 187,706 - GFF179 0.29 -0.6 187,743 + GFF179 0.32 +0.1 187,743 + GFF179 0.32 +0.5 187,744 - GFF179 0.32 -0.4 187,744 - GFF179 0.32 +0.0 187,744 - GFF179 0.32 -0.2 187,744 - GFF179 0.32 +0.1 187,781 + GFF179 0.36 +0.9 187,782 - GFF179 0.36 +0.8 187,926 - GFF179 0.50 +0.8 188,027 - GFF179 0.59 +1.9 188,063 + GFF179 0.63 -0.1 188,127 - GFF179 0.69 -0.7 188,127 - GFF179 0.69 -0.1 188,178 - GFF179 0.74 +1.1 188,274 - GFF179 0.83 +0.0 188,274 - GFF179 0.83 -0.2 188,373 + -0.7 188,374 - -1.1
Or see this region's nucleotide sequence