Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF150 and GFF151 are separated by 186 nucleotides GFF151 and GFF152 overlap by 4 nucleotides
GFF150 - DNA ligase, at 160,443 to 162,074
GFF150
GFF151 - hypothetical protein, at 162,261 to 162,557
GFF151
GFF152 - hypothetical protein, at 162,554 to 163,603
GFF152
Position (kb) 162
163 Strain fitness (log2 ratio) -1
0
1 at 161.285 kb on + strand, within GFF150 at 161.396 kb on + strand, within GFF150 at 161.543 kb on - strand, within GFF150 at 161.848 kb on + strand, within GFF150 at 161.848 kb on + strand, within GFF150 at 161.848 kb on + strand, within GFF150 at 161.848 kb on + strand, within GFF150 at 161.849 kb on - strand, within GFF150 at 161.849 kb on - strand, within GFF150 at 161.849 kb on - strand, within GFF150 at 161.849 kb on - strand, within GFF150 at 161.855 kb on - strand, within GFF150 at 162.037 kb on + strand at 162.037 kb on + strand at 162.037 kb on + strand at 162.038 kb on - strand at 162.283 kb on + strand at 162.284 kb on - strand at 162.284 kb on - strand at 162.379 kb on + strand, within GFF151 at 162.503 kb on - strand, within GFF151 at 162.503 kb on - strand, within GFF151 at 162.628 kb on + strand at 162.904 kb on + strand, within GFF152 at 162.979 kb on - strand, within GFF152 at 162.979 kb on - strand, within GFF152 at 162.981 kb on + strand, within GFF152 at 162.981 kb on + strand, within GFF152 at 162.982 kb on - strand, within GFF152 at 162.982 kb on - strand, within GFF152 at 163.014 kb on + strand, within GFF152 at 163.014 kb on + strand, within GFF152 at 163.014 kb on + strand, within GFF152 at 163.014 kb on + strand, within GFF152 at 163.015 kb on - strand, within GFF152 at 163.015 kb on - strand, within GFF152 at 163.080 kb on + strand, within GFF152 at 163.257 kb on + strand, within GFF152 at 163.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 161,285 + GFF150 0.52 -0.3 161,396 + GFF150 0.58 +0.4 161,543 - GFF150 0.67 -0.6 161,848 + GFF150 0.86 +1.7 161,848 + GFF150 0.86 +0.0 161,848 + GFF150 0.86 +0.2 161,848 + GFF150 0.86 -0.3 161,849 - GFF150 0.86 -0.4 161,849 - GFF150 0.86 -0.2 161,849 - GFF150 0.86 +0.3 161,849 - GFF150 0.86 -0.7 161,855 - GFF150 0.87 -0.2 162,037 + -0.6 162,037 + -0.2 162,037 + +0.3 162,038 - +0.2 162,283 + +0.0 162,284 - -0.3 162,284 - -0.1 162,379 + GFF151 0.40 +1.2 162,503 - GFF151 0.81 -0.1 162,503 - GFF151 0.81 +0.4 162,628 + -0.8 162,904 + GFF152 0.33 -0.7 162,979 - GFF152 0.40 -0.8 162,979 - GFF152 0.40 -0.7 162,981 + GFF152 0.41 -1.4 162,981 + GFF152 0.41 +0.4 162,982 - GFF152 0.41 -0.1 162,982 - GFF152 0.41 -0.5 163,014 + GFF152 0.44 -0.3 163,014 + GFF152 0.44 +0.3 163,014 + GFF152 0.44 -0.4 163,014 + GFF152 0.44 +0.3 163,015 - GFF152 0.44 -0.1 163,015 - GFF152 0.44 +0.3 163,080 + GFF152 0.50 -0.2 163,257 + GFF152 0.67 +0.2 163,539 + -0.4
Or see this region's nucleotide sequence