Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF136 and GFF137 are separated by 206 nucleotides GFF137 and GFF138 are separated by 87 nucleotides GFF138 and GFF139 are separated by 197 nucleotides
GFF136 - Acetyl esterase, at 147,589 to 148,545
GFF136
GFF137 - hypothetical protein, at 148,752 to 149,219
GFF137
GFF138 - 2-hydroxychromene-2-carboxylate isomerase, at 149,307 to 149,951
GFF138
GFF139 - hypothetical protein, at 150,149 to 150,400
GFF139
Position (kb)
148
149
150 Strain fitness (log2 ratio)
-1
0
1 at 147.863 kb on - strand, within GFF136 at 147.971 kb on - strand, within GFF136 at 147.971 kb on - strand, within GFF136 at 148.190 kb on - strand, within GFF136 at 148.280 kb on - strand, within GFF136 at 148.280 kb on - strand, within GFF136 at 148.454 kb on - strand at 148.454 kb on - strand at 148.528 kb on + strand at 148.814 kb on - strand, within GFF137 at 148.963 kb on + strand, within GFF137 at 148.964 kb on - strand, within GFF137 at 148.964 kb on - strand, within GFF137 at 149.270 kb on - strand at 149.407 kb on + strand, within GFF138 at 149.408 kb on - strand, within GFF138 at 149.462 kb on - strand, within GFF138 at 149.564 kb on - strand, within GFF138 at 149.996 kb on + strand at 149.996 kb on + strand at 149.997 kb on - strand at 149.997 kb on - strand at 149.997 kb on - strand at 149.997 kb on - strand at 149.997 kb on - strand at 150.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 147,863 - GFF136 0.29 -0.1 147,971 - GFF136 0.40 +0.4 147,971 - GFF136 0.40 +0.1 148,190 - GFF136 0.63 +0.5 148,280 - GFF136 0.72 -0.4 148,280 - GFF136 0.72 -1.3 148,454 - +0.8 148,454 - +1.5 148,528 + -1.0 148,814 - GFF137 0.13 -0.2 148,963 + GFF137 0.45 +0.2 148,964 - GFF137 0.45 -0.6 148,964 - GFF137 0.45 +0.3 149,270 - -0.5 149,407 + GFF138 0.16 -0.3 149,408 - GFF138 0.16 -0.3 149,462 - GFF138 0.24 -0.5 149,564 - GFF138 0.40 +0.5 149,996 + +0.6 149,996 + -0.8 149,997 - +0.1 149,997 - +0.9 149,997 - -1.2 149,997 - +0.4 149,997 - +1.2 150,148 + -0.2
Or see this region's nucleotide sequence