Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1039 and GFF1040 are separated by 496 nucleotides GFF1040 and GFF1041 are separated by 9 nucleotides GFF1041 and GFF1042 overlap by 4 nucleotides
GFF1039 - Proline--tRNA ligase, at 1,118,812 to 1,120,134
GFF1039
GFF1040 - hypothetical protein, at 1,120,631 to 1,121,074
GFF1040
GFF1041 - hypothetical protein, at 1,121,084 to 1,121,458
GFF1041
GFF1042 - putative cysteine desulfurase, at 1,121,455 to 1,122,702
GFF1042
Position (kb)
1120
1121
1122 Strain fitness (log2 ratio)
-1
0
1
2 at 1120.242 kb on + strand at 1120.304 kb on - strand at 1120.414 kb on - strand at 1120.422 kb on + strand at 1120.422 kb on + strand at 1120.470 kb on + strand at 1120.470 kb on + strand at 1120.471 kb on - strand at 1120.471 kb on - strand at 1120.471 kb on - strand at 1120.635 kb on + strand at 1120.635 kb on + strand at 1120.695 kb on + strand, within GFF1040 at 1120.695 kb on + strand, within GFF1040 at 1120.696 kb on - strand, within GFF1040 at 1120.975 kb on - strand, within GFF1040 at 1120.975 kb on - strand, within GFF1040 at 1121.007 kb on + strand, within GFF1040 at 1121.008 kb on - strand, within GFF1040 at 1121.008 kb on - strand, within GFF1040 at 1121.299 kb on - strand, within GFF1041 at 1121.316 kb on + strand, within GFF1041 at 1121.343 kb on + strand, within GFF1041 at 1121.343 kb on + strand, within GFF1041
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 1,120,242 + -1.0 1,120,304 - +1.7 1,120,414 - +1.3 1,120,422 + +0.9 1,120,422 + +0.4 1,120,470 + +1.2 1,120,470 + +1.8 1,120,471 - +1.2 1,120,471 - +0.9 1,120,471 - +1.3 1,120,635 + +0.6 1,120,635 + -0.4 1,120,695 + GFF1040 0.14 -0.5 1,120,695 + GFF1040 0.14 +0.3 1,120,696 - GFF1040 0.15 +0.7 1,120,975 - GFF1040 0.77 -0.2 1,120,975 - GFF1040 0.77 +0.5 1,121,007 + GFF1040 0.85 +0.3 1,121,008 - GFF1040 0.85 +0.3 1,121,008 - GFF1040 0.85 -0.6 1,121,299 - GFF1041 0.57 +0.8 1,121,316 + GFF1041 0.62 +0.3 1,121,343 + GFF1041 0.69 -0.1 1,121,343 + GFF1041 0.69 +1.3
Or see this region's nucleotide sequence