Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF90 and GFF91 are separated by 222 nucleotides GFF91 and GFF92 are separated by 127 nucleotides GFF92 and GFF93 overlap by 4 nucleotides
GFF90 - Bifunctional PGK/TIM, at 99,001 to 100,194
GFF90
GFF91 - hypothetical protein, at 100,417 to 101,439
GFF91
GFF92 - Thiamine-phosphate synthase, at 101,567 to 102,199
GFF92
GFF93 - hypothetical protein, at 102,196 to 103,353
GFF93
Position (kb) 100
101
102 Strain fitness (log2 ratio) -2
-1
0
1 at 100.559 kb on - strand, within GFF91 at 100.594 kb on + strand, within GFF91 at 100.594 kb on + strand, within GFF91 at 100.595 kb on - strand, within GFF91 at 100.616 kb on - strand, within GFF91 at 100.793 kb on - strand, within GFF91 at 100.864 kb on + strand, within GFF91 at 100.864 kb on + strand, within GFF91 at 100.903 kb on + strand, within GFF91 at 101.035 kb on + strand, within GFF91 at 101.035 kb on + strand, within GFF91 at 101.035 kb on + strand, within GFF91 at 101.036 kb on - strand, within GFF91 at 101.038 kb on + strand, within GFF91 at 101.039 kb on - strand, within GFF91 at 101.039 kb on - strand, within GFF91 at 101.278 kb on + strand, within GFF91 at 101.278 kb on + strand, within GFF91 at 101.278 kb on + strand, within GFF91 at 101.491 kb on + strand at 101.492 kb on - strand at 101.492 kb on - strand at 101.945 kb on + strand, within GFF92
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 100,559 - GFF91 0.14 +0.2 100,594 + GFF91 0.17 -1.5 100,594 + GFF91 0.17 -2.2 100,595 - GFF91 0.17 +0.6 100,616 - GFF91 0.19 +1.0 100,793 - GFF91 0.37 +1.2 100,864 + GFF91 0.44 -1.1 100,864 + GFF91 0.44 -0.5 100,903 + GFF91 0.48 -0.0 101,035 + GFF91 0.60 +1.0 101,035 + GFF91 0.60 -0.1 101,035 + GFF91 0.60 -0.9 101,036 - GFF91 0.61 -1.2 101,038 + GFF91 0.61 +0.0 101,039 - GFF91 0.61 +0.3 101,039 - GFF91 0.61 +0.2 101,278 + GFF91 0.84 -0.0 101,278 + GFF91 0.84 -0.5 101,278 + GFF91 0.84 +0.5 101,491 + -0.3 101,492 - -0.6 101,492 - -0.5 101,945 + GFF92 0.60 -0.5
Or see this region's nucleotide sequence