Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF731 and GFF732 are separated by 125 nucleotides GFF732 and GFF733 are separated by 1 nucleotides GFF733 and GFF734 overlap by 4 nucleotides
GFF731 - Leu/Ile/Val-binding protein, at 777,356 to 778,456
GFF731
GFF732 - hypothetical protein, at 778,582 to 779,040
GFF732
GFF733 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 779,042 to 779,815
GFF733
GFF734 - putative amino-acid import ATP-binding protein YxeO, at 779,812 to 780,756
GFF734
Position (kb) 778
779
780 Strain fitness (log2 ratio) -1
0
1 at 777.784 kb on - strand, within GFF731 at 777.846 kb on + strand, within GFF731 at 777.846 kb on + strand, within GFF731 at 777.877 kb on - strand, within GFF731 at 777.881 kb on - strand, within GFF731 at 777.942 kb on + strand, within GFF731 at 778.000 kb on - strand, within GFF731 at 778.000 kb on - strand, within GFF731 at 778.125 kb on + strand, within GFF731 at 778.126 kb on - strand, within GFF731 at 778.162 kb on - strand, within GFF731 at 778.162 kb on - strand, within GFF731 at 778.634 kb on + strand, within GFF732 at 778.634 kb on + strand, within GFF732 at 778.634 kb on + strand, within GFF732 at 778.634 kb on + strand, within GFF732 at 778.646 kb on + strand, within GFF732 at 778.646 kb on + strand, within GFF732 at 778.647 kb on - strand, within GFF732 at 778.806 kb on - strand, within GFF732 at 778.806 kb on - strand, within GFF732 at 778.823 kb on + strand, within GFF732 at 779.061 kb on + strand at 779.139 kb on + strand, within GFF733 at 779.139 kb on + strand, within GFF733 at 779.575 kb on - strand, within GFF733 at 779.722 kb on - strand, within GFF733 at 779.725 kb on - strand, within GFF733 at 779.867 kb on + strand at 779.867 kb on + strand at 779.868 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 777,784 - GFF731 0.39 +0.6 777,846 + GFF731 0.45 +0.0 777,846 + GFF731 0.45 +0.7 777,877 - GFF731 0.47 -0.3 777,881 - GFF731 0.48 +1.1 777,942 + GFF731 0.53 +0.7 778,000 - GFF731 0.58 +0.4 778,000 - GFF731 0.58 +1.5 778,125 + GFF731 0.70 +1.2 778,126 - GFF731 0.70 +1.5 778,162 - GFF731 0.73 +1.7 778,162 - GFF731 0.73 +0.1 778,634 + GFF732 0.11 -1.4 778,634 + GFF732 0.11 -0.9 778,634 + GFF732 0.11 +0.1 778,634 + GFF732 0.11 -0.3 778,646 + GFF732 0.14 -1.4 778,646 + GFF732 0.14 -0.0 778,647 - GFF732 0.14 +0.3 778,806 - GFF732 0.49 +1.4 778,806 - GFF732 0.49 +0.1 778,823 + GFF732 0.53 +0.3 779,061 + +0.1 779,139 + GFF733 0.13 +0.6 779,139 + GFF733 0.13 +1.0 779,575 - GFF733 0.69 -0.4 779,722 - GFF733 0.88 +0.1 779,725 - GFF733 0.88 +0.5 779,867 + +0.4 779,867 + +0.9 779,868 - +0.8
Or see this region's nucleotide sequence