Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF718 and GFF719 are separated by 10 nucleotides GFF719 and GFF720 are separated by 5 nucleotides GFF720 and GFF721 overlap by 4 nucleotides GFF721 and GFF722 are separated by 31 nucleotides
GFF718 - Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha, at 766,226 to 767,236
GFF718
GFF719 - hypothetical protein, at 767,247 to 767,603
GFF719
GFF720 - 1-deoxy-D-xylulose-5-phosphate synthase, at 767,609 to 769,000
GFF720
GFF721 - hypothetical protein, at 768,997 to 769,704
GFF721
GFF722 - Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, at 769,736 to 771,103
GFF722
Position (kb) 767
768
769
770 Strain fitness (log2 ratio) -2
-1
0
1 at 766.668 kb on - strand, within GFF718 at 766.778 kb on + strand, within GFF718 at 766.800 kb on - strand, within GFF718 at 766.986 kb on - strand, within GFF718 at 767.266 kb on + strand at 767.382 kb on + strand, within GFF719 at 768.263 kb on - strand, within GFF720 at 768.326 kb on + strand, within GFF720 at 768.417 kb on - strand, within GFF720 at 768.549 kb on - strand, within GFF720 at 768.665 kb on + strand, within GFF720 at 768.666 kb on - strand, within GFF720 at 768.992 kb on + strand at 769.039 kb on + strand at 769.039 kb on + strand at 769.039 kb on + strand at 769.040 kb on - strand at 769.243 kb on + strand, within GFF721 at 769.392 kb on + strand, within GFF721 at 769.429 kb on + strand, within GFF721 at 769.430 kb on - strand, within GFF721 at 769.587 kb on + strand, within GFF721 at 769.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 766,668 - GFF718 0.44 -2.1 766,778 + GFF718 0.55 +0.3 766,800 - GFF718 0.57 -0.0 766,986 - GFF718 0.75 -0.9 767,266 + -0.1 767,382 + GFF719 0.38 -1.1 768,263 - GFF720 0.47 -0.2 768,326 + GFF720 0.52 -0.8 768,417 - GFF720 0.58 +1.0 768,549 - GFF720 0.68 -0.6 768,665 + GFF720 0.76 +0.7 768,666 - GFF720 0.76 -0.4 768,992 + +0.2 769,039 + -0.6 769,039 + +0.3 769,039 + -0.7 769,040 - -0.6 769,243 + GFF721 0.35 +1.0 769,392 + GFF721 0.56 -1.0 769,429 + GFF721 0.61 +0.6 769,430 - GFF721 0.61 +1.6 769,587 + GFF721 0.83 +0.5 769,639 + +0.1
Or see this region's nucleotide sequence