Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF63 and GFF64 are separated by 171 nucleotides GFF64 and GFF65 are separated by 18 nucleotides GFF65 and GFF66 are separated by 9 nucleotides
GFF63 - Cytochrome b/c1, at 72,965 to 73,810
GFF63
GFF64 - Adenine phosphoribosyltransferase, at 73,982 to 74,518
GFF64
GFF65 - hypothetical protein, at 74,537 to 75,241
GFF65
GFF66 - Methionine aminopeptidase, at 75,251 to 76,078
GFF66
Position (kb) 74
75
76 Strain fitness (log2 ratio) -1
0
1
2 at 73.955 kb on - strand at 74.028 kb on - strand at 74.754 kb on + strand, within GFF65 at 74.856 kb on + strand, within GFF65 at 74.857 kb on - strand, within GFF65 at 74.877 kb on + strand, within GFF65 at 74.877 kb on + strand, within GFF65 at 74.921 kb on + strand, within GFF65 at 75.264 kb on + strand at 75.264 kb on + strand at 75.264 kb on + strand at 75.264 kb on + strand at 75.264 kb on + strand at 75.264 kb on + strand at 75.265 kb on - strand at 75.279 kb on + strand at 75.654 kb on + strand, within GFF66 at 75.654 kb on + strand, within GFF66 at 75.855 kb on + strand, within GFF66 at 75.873 kb on + strand, within GFF66 at 76.071 kb on + strand at 76.133 kb on + strand at 76.134 kb on - strand at 76.141 kb on + strand at 76.141 kb on + strand at 76.141 kb on + strand at 76.142 kb on - strand at 76.142 kb on - strand at 76.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 73,955 - -0.4 74,028 - -0.4 74,754 + GFF65 0.31 +0.3 74,856 + GFF65 0.45 -0.7 74,857 - GFF65 0.45 +0.8 74,877 + GFF65 0.48 -0.5 74,877 + GFF65 0.48 +1.0 74,921 + GFF65 0.54 +0.8 75,264 + -0.5 75,264 + +0.4 75,264 + -0.0 75,264 + -0.8 75,264 + -0.5 75,264 + -0.3 75,265 - -0.0 75,279 + -0.4 75,654 + GFF66 0.49 -0.8 75,654 + GFF66 0.49 +0.1 75,855 + GFF66 0.73 -1.0 75,873 + GFF66 0.75 -1.7 76,071 + -0.4 76,133 + +1.8 76,134 - +0.1 76,141 + -0.4 76,141 + -0.3 76,141 + -0.3 76,142 - +0.6 76,142 - -1.2 76,142 - +0.7
Or see this region's nucleotide sequence