Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF45 and GFF46 are separated by 9 nucleotides GFF46 and GFF47 overlap by 4 nucleotides GFF47 and GFF48 overlap by 154 nucleotides GFF48 and GFF49 are separated by 99 nucleotides
GFF45 - Spermidine/putrescine import ATP-binding protein PotA, at 54,200 to 55,378
GFF45
GFF46 - Putrescine transport system permease protein PotH, at 55,388 to 56,305
GFF46
GFF47 - Inner membrane ABC transporter permease protein YdcV, at 56,302 to 57,117
GFF47
GFF48 - hypothetical protein, at 56,964 to 57,383
GFF48
GFF49 - hypothetical protein, at 57,483 to 59,549
GFF49
Position (kb) 56
57
58 Strain fitness (log2 ratio) -1
0
1 at 55.376 kb on + strand at 55.376 kb on + strand at 55.377 kb on - strand at 55.442 kb on + strand at 55.443 kb on - strand at 55.443 kb on - strand at 55.529 kb on + strand, within GFF46 at 55.604 kb on + strand, within GFF46 at 55.610 kb on + strand, within GFF46 at 55.611 kb on - strand, within GFF46 at 55.611 kb on - strand, within GFF46 at 55.646 kb on + strand, within GFF46 at 55.715 kb on + strand, within GFF46 at 55.716 kb on - strand, within GFF46 at 55.913 kb on + strand, within GFF46 at 55.914 kb on - strand, within GFF46 at 55.928 kb on + strand, within GFF46 at 55.928 kb on + strand, within GFF46 at 55.935 kb on - strand, within GFF46 at 55.962 kb on - strand, within GFF46 at 55.962 kb on - strand, within GFF46 at 56.265 kb on - strand at 56.389 kb on + strand, within GFF47 at 56.390 kb on - strand, within GFF47 at 56.447 kb on - strand, within GFF47 at 56.478 kb on - strand, within GFF47 at 56.611 kb on + strand, within GFF47 at 56.612 kb on - strand, within GFF47 at 56.965 kb on + strand at 56.965 kb on + strand at 57.148 kb on - strand, within GFF48 at 57.439 kb on + strand at 57.439 kb on + strand at 57.440 kb on - strand at 57.440 kb on - strand at 57.440 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 55,376 + +1.2 55,376 + -0.3 55,377 - -1.3 55,442 + +0.1 55,443 - -0.9 55,443 - -0.3 55,529 + GFF46 0.15 -0.9 55,604 + GFF46 0.24 +0.4 55,610 + GFF46 0.24 -0.8 55,611 - GFF46 0.24 -0.7 55,611 - GFF46 0.24 -0.4 55,646 + GFF46 0.28 +0.2 55,715 + GFF46 0.36 +0.6 55,716 - GFF46 0.36 +0.1 55,913 + GFF46 0.57 -0.1 55,914 - GFF46 0.57 -1.1 55,928 + GFF46 0.59 -0.5 55,928 + GFF46 0.59 -0.2 55,935 - GFF46 0.60 -0.8 55,962 - GFF46 0.63 -0.1 55,962 - GFF46 0.63 -1.4 56,265 - -0.6 56,389 + GFF47 0.11 -1.2 56,390 - GFF47 0.11 -0.6 56,447 - GFF47 0.18 +0.1 56,478 - GFF47 0.22 +0.4 56,611 + GFF47 0.38 -0.2 56,612 - GFF47 0.38 -1.2 56,965 + -1.7 56,965 + -0.1 57,148 - GFF48 0.44 -0.3 57,439 + -0.2 57,439 + -0.5 57,440 - -0.0 57,440 - +0.4 57,440 - +0.1
Or see this region's nucleotide sequence