Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4634 and GFF4635 are separated by 221 nucleotides GFF4635 and GFF4636 are separated by 203 nucleotides GFF4636 and GFF4637 are separated by 98 nucleotides
GFF4634 - O-succinylhomoserine sulfhydrylase, at 43,198 to 44,418
GFF4634
GFF4635 - dCTP deaminase, dUMP-forming, at 44,640 to 45,767
GFF4635
GFF4636 - hypothetical protein, at 45,971 to 46,357
GFF4636
GFF4637 - hypothetical protein, at 46,456 to 48,687
GFF4637
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1 at 43.769 kb on + strand, within GFF4634 at 43.770 kb on - strand, within GFF4634 at 43.770 kb on - strand, within GFF4634 at 44.270 kb on + strand, within GFF4634 at 44.344 kb on + strand at 44.344 kb on + strand at 44.368 kb on - strand at 44.511 kb on + strand at 44.512 kb on - strand at 45.787 kb on - strand at 45.787 kb on - strand at 45.962 kb on + strand at 46.042 kb on + strand, within GFF4636 at 46.043 kb on - strand, within GFF4636 at 46.043 kb on - strand, within GFF4636 at 46.063 kb on + strand, within GFF4636 at 46.312 kb on + strand, within GFF4636 at 46.313 kb on - strand, within GFF4636 at 46.425 kb on + strand at 46.425 kb on + strand at 46.426 kb on - strand at 46.426 kb on - strand at 46.580 kb on + strand at 46.581 kb on - strand at 46.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 43,769 + GFF4634 0.47 +0.2 43,770 - GFF4634 0.47 +0.3 43,770 - GFF4634 0.47 +0.6 44,270 + GFF4634 0.88 +0.0 44,344 + +0.5 44,344 + +0.3 44,368 - -0.3 44,511 + -0.1 44,512 - +0.1 45,787 - +0.2 45,787 - +1.3 45,962 + +0.5 46,042 + GFF4636 0.18 +0.2 46,043 - GFF4636 0.19 +0.5 46,043 - GFF4636 0.19 -0.2 46,063 + GFF4636 0.24 +0.6 46,312 + GFF4636 0.88 -0.9 46,313 - GFF4636 0.88 +0.0 46,425 + +0.6 46,425 + -0.1 46,426 - +0.2 46,426 - -1.8 46,580 + +0.4 46,581 - +0.5 46,581 - -0.7
Or see this region's nucleotide sequence