Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4541 and GFF4542 are separated by 472 nucleotides GFF4542 and GFF4543 are separated by 164 nucleotides GFF4543 and GFF4544 overlap by 4 nucleotides
GFF4541 - Coniferyl aldehyde dehydrogenase, at 120,131 to 121,423
GFF4541
GFF4542 - Riboflavin-binding protein RibY, at 121,896 to 122,891
GFF4542
GFF4543 - Shikimate dehydrogenase (NADP(+)), at 123,056 to 123,859
GFF4543
GFF4544 - 2-oxo-hept-4-ene-1,7-dioate hydratase, at 123,856 to 124,644
GFF4544
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-2
-1
0
1 at 121.179 kb on + strand, within GFF4541 at 121.180 kb on - strand, within GFF4541 at 121.230 kb on + strand, within GFF4541 at 121.230 kb on + strand, within GFF4541 at 121.230 kb on + strand, within GFF4541 at 121.230 kb on + strand, within GFF4541 at 121.230 kb on + strand, within GFF4541 at 121.331 kb on + strand at 121.385 kb on + strand at 121.519 kb on + strand at 121.520 kb on - strand at 121.660 kb on - strand at 121.668 kb on + strand at 121.668 kb on + strand at 121.669 kb on - strand at 121.669 kb on - strand at 121.669 kb on - strand at 121.757 kb on + strand at 121.757 kb on + strand at 121.763 kb on + strand at 121.892 kb on + strand at 121.945 kb on + strand at 121.945 kb on + strand at 122.198 kb on + strand, within GFF4542 at 122.199 kb on - strand, within GFF4542 at 122.304 kb on + strand, within GFF4542 at 122.306 kb on + strand, within GFF4542 at 122.348 kb on - strand, within GFF4542 at 122.348 kb on - strand, within GFF4542 at 122.666 kb on + strand, within GFF4542 at 122.694 kb on - strand, within GFF4542 at 122.823 kb on + strand at 122.901 kb on - strand at 122.996 kb on - strand at 123.055 kb on + strand at 123.055 kb on + strand at 123.055 kb on + strand at 123.055 kb on + strand at 123.056 kb on - strand at 123.056 kb on - strand at 123.080 kb on + strand at 123.080 kb on + strand at 123.081 kb on - strand at 123.081 kb on - strand at 123.137 kb on + strand, within GFF4543 at 123.137 kb on + strand, within GFF4543 at 123.138 kb on - strand, within GFF4543 at 123.138 kb on - strand, within GFF4543 at 123.289 kb on - strand, within GFF4543 at 123.289 kb on - strand, within GFF4543 at 123.441 kb on - strand, within GFF4543 at 123.441 kb on - strand, within GFF4543 at 123.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 121,179 + GFF4541 0.81 +0.1 121,180 - GFF4541 0.81 -0.7 121,230 + GFF4541 0.85 -0.4 121,230 + GFF4541 0.85 -0.4 121,230 + GFF4541 0.85 +0.1 121,230 + GFF4541 0.85 +0.3 121,230 + GFF4541 0.85 -0.3 121,331 + -0.1 121,385 + -0.4 121,519 + -0.9 121,520 - -0.1 121,660 - -0.1 121,668 + -0.2 121,668 + -0.4 121,669 - -0.3 121,669 - -0.5 121,669 - +0.5 121,757 + -0.3 121,757 + +0.2 121,763 + +0.5 121,892 + +0.4 121,945 + -2.2 121,945 + +0.0 122,198 + GFF4542 0.30 +0.3 122,199 - GFF4542 0.30 +1.3 122,304 + GFF4542 0.41 -1.1 122,306 + GFF4542 0.41 -1.0 122,348 - GFF4542 0.45 +0.6 122,348 - GFF4542 0.45 -0.2 122,666 + GFF4542 0.77 +0.6 122,694 - GFF4542 0.80 -0.6 122,823 + -1.5 122,901 - +0.1 122,996 - +0.9 123,055 + -1.1 123,055 + -0.2 123,055 + -0.2 123,055 + +0.6 123,056 - +0.5 123,056 - -0.8 123,080 + -0.2 123,080 + +0.2 123,081 - -0.8 123,081 - +0.1 123,137 + GFF4543 0.10 +0.8 123,137 + GFF4543 0.10 +0.2 123,138 - GFF4543 0.10 +0.1 123,138 - GFF4543 0.10 +0.1 123,289 - GFF4543 0.29 -0.2 123,289 - GFF4543 0.29 -0.6 123,441 - GFF4543 0.48 -1.0 123,441 - GFF4543 0.48 +1.3 123,828 - -0.3
Or see this region's nucleotide sequence