Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4411 and GFF4412 are separated by 719 nucleotides GFF4412 and GFF4413 are separated by 39 nucleotides GFF4413 and GFF4414 are separated by 3 nucleotides
GFF4411 - Malate dehydrogenase, at 217,956 to 218,921
GFF4411
GFF4412 - Nitrogenase-stabilizing/protective protein NifW, at 219,641 to 219,982
GFF4412
GFF4413 - Serine acetyltransferase, at 220,022 to 220,801
GFF4413
GFF4414 - 2-phosphonomethylmalate synthase, at 220,805 to 222,052
GFF4414
Position (kb) 219
220 Strain fitness (log2 ratio) -1
0
1 at 218.726 kb on - strand, within GFF4411 at 218.991 kb on - strand at 219.034 kb on + strand at 219.034 kb on + strand at 219.035 kb on - strand at 219.237 kb on + strand at 219.238 kb on - strand at 219.278 kb on + strand at 219.279 kb on - strand at 219.279 kb on - strand at 219.359 kb on - strand at 219.505 kb on - strand at 219.524 kb on - strand at 219.524 kb on - strand at 219.524 kb on - strand at 219.524 kb on - strand at 219.524 kb on - strand at 219.777 kb on + strand, within GFF4412 at 219.778 kb on - strand, within GFF4412 at 219.778 kb on - strand, within GFF4412 at 219.778 kb on - strand, within GFF4412 at 219.778 kb on - strand, within GFF4412 at 219.778 kb on - strand, within GFF4412 at 219.778 kb on - strand, within GFF4412 at 219.810 kb on + strand, within GFF4412 at 219.811 kb on - strand, within GFF4412 at 219.811 kb on - strand, within GFF4412 at 219.855 kb on + strand, within GFF4412 at 219.856 kb on - strand, within GFF4412 at 219.856 kb on - strand, within GFF4412 at 219.856 kb on - strand, within GFF4412 at 219.912 kb on + strand, within GFF4412 at 220.421 kb on + strand, within GFF4413 at 220.630 kb on - strand, within GFF4413 at 220.772 kb on - strand at 220.849 kb on - strand at 220.866 kb on + strand at 220.866 kb on + strand at 220.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 218,726 - GFF4411 0.80 +0.6 218,991 - -0.4 219,034 + -1.2 219,034 + +0.3 219,035 - +0.5 219,237 + +0.3 219,238 - -1.0 219,278 + -0.5 219,279 - -0.3 219,279 - +0.2 219,359 - +0.6 219,505 - -0.9 219,524 - +0.6 219,524 - +0.6 219,524 - -0.1 219,524 - -0.1 219,524 - -0.9 219,777 + GFF4412 0.40 +0.8 219,778 - GFF4412 0.40 +0.0 219,778 - GFF4412 0.40 +0.3 219,778 - GFF4412 0.40 +0.0 219,778 - GFF4412 0.40 +0.8 219,778 - GFF4412 0.40 -0.5 219,778 - GFF4412 0.40 -0.1 219,810 + GFF4412 0.49 -0.4 219,811 - GFF4412 0.50 +0.1 219,811 - GFF4412 0.50 +0.6 219,855 + GFF4412 0.63 +0.3 219,856 - GFF4412 0.63 -1.5 219,856 - GFF4412 0.63 +0.0 219,856 - GFF4412 0.63 -0.2 219,912 + GFF4412 0.79 +0.2 220,421 + GFF4413 0.51 -0.2 220,630 - GFF4413 0.78 -1.4 220,772 - -1.3 220,849 - +0.0 220,866 + +0.1 220,866 + -1.0 220,867 - -0.7
Or see this region's nucleotide sequence