Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4330 and GFF4331 overlap by 4 nucleotides GFF4331 and GFF4332 overlap by 4 nucleotides GFF4332 and GFF4333 are separated by 14 nucleotides
GFF4330 - putative acetyl-CoA acyltransferase, at 113,222 to 114,349
GFF4330
GFF4331 - putative acyl--CoA ligase YhfT, at 114,346 to 115,806
GFF4331
GFF4332 - Biotin transporter BioY, at 115,803 to 116,378
GFF4332
GFF4333 - Energy-coupling factor transporter transmembrane protein BioN, at 116,393 to 117,025
GFF4333
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-1
0
1
2 at 113.469 kb on + strand, within GFF4330 at 113.633 kb on + strand, within GFF4330 at 113.781 kb on - strand, within GFF4330 at 114.033 kb on + strand, within GFF4330 at 114.034 kb on - strand, within GFF4330 at 114.433 kb on + strand at 114.752 kb on + strand, within GFF4331 at 114.752 kb on + strand, within GFF4331 at 114.752 kb on + strand, within GFF4331 at 114.753 kb on - strand, within GFF4331 at 114.753 kb on - strand, within GFF4331 at 114.753 kb on - strand, within GFF4331 at 114.779 kb on + strand, within GFF4331 at 114.793 kb on + strand, within GFF4331 at 114.960 kb on - strand, within GFF4331 at 115.121 kb on + strand, within GFF4331 at 115.122 kb on - strand, within GFF4331 at 115.229 kb on + strand, within GFF4331 at 115.229 kb on + strand, within GFF4331 at 115.229 kb on + strand, within GFF4331 at 115.230 kb on - strand, within GFF4331 at 115.230 kb on - strand, within GFF4331 at 115.388 kb on + strand, within GFF4331 at 115.388 kb on + strand, within GFF4331 at 115.388 kb on + strand, within GFF4331 at 115.389 kb on - strand, within GFF4331 at 115.389 kb on - strand, within GFF4331 at 115.721 kb on + strand at 115.721 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 113,469 + GFF4330 0.22 -0.5 113,633 + GFF4330 0.36 -0.2 113,781 - GFF4330 0.50 -0.8 114,033 + GFF4330 0.72 +0.7 114,034 - GFF4330 0.72 -0.0 114,433 + -0.4 114,752 + GFF4331 0.28 +0.7 114,752 + GFF4331 0.28 +0.5 114,752 + GFF4331 0.28 +0.3 114,753 - GFF4331 0.28 +0.4 114,753 - GFF4331 0.28 +0.2 114,753 - GFF4331 0.28 -0.0 114,779 + GFF4331 0.30 -0.6 114,793 + GFF4331 0.31 -0.3 114,960 - GFF4331 0.42 +0.7 115,121 + GFF4331 0.53 -0.5 115,122 - GFF4331 0.53 -0.6 115,229 + GFF4331 0.60 -0.6 115,229 + GFF4331 0.60 +1.9 115,229 + GFF4331 0.60 +0.0 115,230 - GFF4331 0.61 +0.2 115,230 - GFF4331 0.61 -0.2 115,388 + GFF4331 0.71 +0.4 115,388 + GFF4331 0.71 +1.2 115,388 + GFF4331 0.71 +0.4 115,389 - GFF4331 0.71 +1.0 115,389 - GFF4331 0.71 +0.7 115,721 + -0.2 115,721 + +0.8
Or see this region's nucleotide sequence