Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF388 and GFF389 are separated by 6 nucleotides GFF389 and GFF390 are separated by 104 nucleotides GFF390 and GFF391 overlap by 10 nucleotides GFF391 and GFF392 are separated by 93 nucleotides
GFF388 - hypothetical protein, at 399,261 to 400,082
GFF388
GFF389 - Acylphosphatase, at 400,089 to 400,379
GFF389
GFF390 - hypothetical protein, at 400,484 to 402,016
GFF390
GFF391 - Aminoalkylphosphonate N-acetyltransferase, at 402,007 to 402,495
GFF391
GFF392 - Disulfide-bond oxidoreductase YfcG, at 402,589 to 403,302
GFF392
Position (kb)
400
401
402
403 Strain fitness (log2 ratio)
-1
0
1
2 at 399.561 kb on + strand, within GFF388 at 399.562 kb on - strand, within GFF388 at 399.762 kb on + strand, within GFF388 at 399.763 kb on - strand, within GFF388 at 399.763 kb on - strand, within GFF388 at 399.778 kb on - strand, within GFF388 at 399.897 kb on + strand, within GFF388 at 399.898 kb on - strand, within GFF388 at 399.898 kb on - strand, within GFF388 at 399.951 kb on + strand, within GFF388 at 399.951 kb on + strand, within GFF388 at 399.951 kb on + strand, within GFF388 at 399.951 kb on + strand, within GFF388 at 399.952 kb on - strand, within GFF388 at 399.972 kb on + strand, within GFF388 at 400.008 kb on + strand at 400.008 kb on + strand at 400.009 kb on - strand at 400.080 kb on + strand at 400.081 kb on - strand at 400.095 kb on + strand at 400.095 kb on + strand at 400.096 kb on - strand at 400.096 kb on - strand at 400.378 kb on - strand at 400.457 kb on + strand at 400.458 kb on - strand at 400.459 kb on + strand at 400.459 kb on + strand at 400.459 kb on + strand at 400.460 kb on - strand at 400.460 kb on - strand at 400.496 kb on + strand at 400.496 kb on + strand at 400.497 kb on - strand at 400.668 kb on - strand, within GFF390 at 400.668 kb on - strand, within GFF390 at 400.694 kb on + strand, within GFF390 at 400.694 kb on + strand, within GFF390 at 400.694 kb on + strand, within GFF390 at 400.694 kb on + strand, within GFF390 at 400.695 kb on - strand, within GFF390 at 400.695 kb on - strand, within GFF390 at 400.695 kb on - strand, within GFF390 at 400.821 kb on - strand, within GFF390 at 400.821 kb on - strand, within GFF390 at 401.010 kb on - strand, within GFF390 at 401.180 kb on + strand, within GFF390 at 401.180 kb on + strand, within GFF390 at 401.181 kb on - strand, within GFF390 at 401.300 kb on + strand, within GFF390 at 401.346 kb on - strand, within GFF390 at 401.346 kb on - strand, within GFF390 at 401.438 kb on - strand, within GFF390 at 401.519 kb on + strand, within GFF390 at 401.519 kb on + strand, within GFF390 at 401.520 kb on - strand, within GFF390 at 401.520 kb on - strand, within GFF390 at 401.520 kb on - strand, within GFF390 at 401.573 kb on + strand, within GFF390 at 401.573 kb on + strand, within GFF390 at 401.573 kb on + strand, within GFF390 at 401.574 kb on - strand, within GFF390 at 401.574 kb on - strand, within GFF390 at 401.625 kb on + strand, within GFF390 at 401.625 kb on + strand, within GFF390 at 401.625 kb on + strand, within GFF390 at 401.684 kb on + strand, within GFF390 at 401.685 kb on - strand, within GFF390 at 402.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 399,561 + GFF388 0.36 +0.6 399,562 - GFF388 0.37 +0.4 399,762 + GFF388 0.61 +2.0 399,763 - GFF388 0.61 +0.4 399,763 - GFF388 0.61 +0.2 399,778 - GFF388 0.63 +0.1 399,897 + GFF388 0.77 +0.1 399,898 - GFF388 0.77 -1.1 399,898 - GFF388 0.77 +1.2 399,951 + GFF388 0.84 -0.3 399,951 + GFF388 0.84 -0.1 399,951 + GFF388 0.84 +0.3 399,951 + GFF388 0.84 -0.9 399,952 - GFF388 0.84 +0.2 399,972 + GFF388 0.86 -0.9 400,008 + +0.2 400,008 + -0.8 400,009 - +1.7 400,080 + -0.1 400,081 - -0.1 400,095 + -0.6 400,095 + +0.0 400,096 - +0.4 400,096 - -0.3 400,378 - +1.1 400,457 + -0.4 400,458 - +0.7 400,459 + +0.1 400,459 + +1.2 400,459 + -0.8 400,460 - -0.8 400,460 - +0.4 400,496 + +0.7 400,496 + -0.4 400,497 - +0.0 400,668 - GFF390 0.12 +0.9 400,668 - GFF390 0.12 -0.1 400,694 + GFF390 0.14 +0.4 400,694 + GFF390 0.14 +0.5 400,694 + GFF390 0.14 +0.4 400,694 + GFF390 0.14 +0.7 400,695 - GFF390 0.14 +0.1 400,695 - GFF390 0.14 +0.7 400,695 - GFF390 0.14 +0.4 400,821 - GFF390 0.22 +0.3 400,821 - GFF390 0.22 +0.2 401,010 - GFF390 0.34 +0.3 401,180 + GFF390 0.45 +0.6 401,180 + GFF390 0.45 +0.5 401,181 - GFF390 0.45 -1.0 401,300 + GFF390 0.53 +0.4 401,346 - GFF390 0.56 +0.5 401,346 - GFF390 0.56 +1.1 401,438 - GFF390 0.62 +0.1 401,519 + GFF390 0.68 -0.6 401,519 + GFF390 0.68 -0.1 401,520 - GFF390 0.68 +0.2 401,520 - GFF390 0.68 +0.4 401,520 - GFF390 0.68 -0.2 401,573 + GFF390 0.71 -0.4 401,573 + GFF390 0.71 -0.0 401,573 + GFF390 0.71 +2.0 401,574 - GFF390 0.71 +0.2 401,574 - GFF390 0.71 -0.0 401,625 + GFF390 0.74 -0.8 401,625 + GFF390 0.74 -0.3 401,625 + GFF390 0.74 +0.9 401,684 + GFF390 0.78 -1.0 401,685 - GFF390 0.78 +1.0 402,514 - -1.0
Or see this region's nucleotide sequence