Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3734 and GFF3735 are separated by 336 nucleotides GFF3735 and GFF3736 are separated by 123 nucleotides GFF3736 and GFF3737 overlap by 4 nucleotides
GFF3734 - Uroporphyrinogen decarboxylase, at 75,549 to 76,595
GFF3734
GFF3735 - Putative pyruvate, phosphate dikinase regulatory protein, at 76,932 to 77,786
GFF3735
GFF3736 - 7-methyl-GTP pyrophosphatase, at 77,910 to 78,509
GFF3736
GFF3737 - Shikimate dehydrogenase (NADP(+)), at 78,506 to 79,354
GFF3737
Position (kb) 76
77
78 Strain fitness (log2 ratio) -2
-1
0
1 at 76.621 kb on - strand at 76.621 kb on - strand at 76.959 kb on + strand at 76.960 kb on - strand at 77.064 kb on + strand, within GFF3735 at 77.142 kb on - strand, within GFF3735 at 77.248 kb on - strand, within GFF3735 at 77.248 kb on - strand, within GFF3735 at 77.248 kb on - strand, within GFF3735 at 77.304 kb on + strand, within GFF3735 at 77.409 kb on - strand, within GFF3735 at 77.433 kb on + strand, within GFF3735 at 77.434 kb on - strand, within GFF3735 at 77.504 kb on - strand, within GFF3735 at 77.619 kb on + strand, within GFF3735 at 77.620 kb on - strand, within GFF3735 at 77.620 kb on - strand, within GFF3735 at 77.620 kb on - strand, within GFF3735 at 77.748 kb on + strand at 77.749 kb on - strand at 77.749 kb on - strand at 78.166 kb on + strand, within GFF3736 at 78.168 kb on + strand, within GFF3736 at 78.168 kb on + strand, within GFF3736
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 76,621 - +1.3 76,621 - +0.1 76,959 + +0.5 76,960 - +0.3 77,064 + GFF3735 0.15 +0.5 77,142 - GFF3735 0.25 -0.9 77,248 - GFF3735 0.37 +0.1 77,248 - GFF3735 0.37 +0.6 77,248 - GFF3735 0.37 +1.0 77,304 + GFF3735 0.44 +0.2 77,409 - GFF3735 0.56 +0.1 77,433 + GFF3735 0.59 -1.8 77,434 - GFF3735 0.59 +1.4 77,504 - GFF3735 0.67 +0.4 77,619 + GFF3735 0.80 +0.2 77,620 - GFF3735 0.80 +1.2 77,620 - GFF3735 0.80 -0.0 77,620 - GFF3735 0.80 +1.0 77,748 + -0.4 77,749 - +0.4 77,749 - +0.5 78,166 + GFF3736 0.43 +0.6 78,168 + GFF3736 0.43 -0.0 78,168 + GFF3736 0.43 +0.3
Or see this region's nucleotide sequence