Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3460 and GFF3461 are separated by 253 nucleotides GFF3461 and GFF3462 are separated by 148 nucleotides GFF3462 and GFF3463 are separated by 380 nucleotides
GFF3460 - Alcohol dehydrogenase [acceptor], at 312,365 to 313,969
GFF3460
GFF3461 - Translation initiation factor IF-1, at 314,223 to 314,498
GFF3461
GFF3462 - ATP-dependent RNA helicase RhlE, at 314,647 to 316,359
GFF3462
GFF3463 - Inner membrane protein YabI, at 316,740 to 317,264
GFF3463
Position (kb) 314
315
316
317 Strain fitness (log2 ratio) -1
0
1 at 313.683 kb on - strand, within GFF3460 at 313.769 kb on + strand, within GFF3460 at 313.769 kb on + strand, within GFF3460 at 313.769 kb on + strand, within GFF3460 at 313.770 kb on - strand, within GFF3460 at 313.770 kb on - strand, within GFF3460 at 313.945 kb on + strand at 314.547 kb on - strand at 314.547 kb on - strand at 314.547 kb on - strand at 315.302 kb on + strand, within GFF3462 at 315.302 kb on + strand, within GFF3462 at 315.303 kb on - strand, within GFF3462 at 315.303 kb on - strand, within GFF3462 at 316.396 kb on - strand at 316.396 kb on - strand at 316.541 kb on + strand at 316.651 kb on + strand at 316.652 kb on - strand at 316.677 kb on + strand at 316.798 kb on - strand, within GFF3463 at 316.980 kb on + strand, within GFF3463 at 316.998 kb on + strand, within GFF3463 at 316.999 kb on - strand, within GFF3463
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 313,683 - GFF3460 0.82 -0.3 313,769 + GFF3460 0.87 +0.4 313,769 + GFF3460 0.87 -0.4 313,769 + GFF3460 0.87 +0.3 313,770 - GFF3460 0.88 +0.0 313,770 - GFF3460 0.88 +0.3 313,945 + -0.9 314,547 - -0.1 314,547 - +0.3 314,547 - +0.6 315,302 + GFF3462 0.38 +0.2 315,302 + GFF3462 0.38 -0.7 315,303 - GFF3462 0.38 +0.5 315,303 - GFF3462 0.38 -1.5 316,396 - +0.1 316,396 - -0.7 316,541 + -0.2 316,651 + -1.0 316,652 - +0.6 316,677 + -0.9 316,798 - GFF3463 0.11 +0.7 316,980 + GFF3463 0.46 +1.0 316,998 + GFF3463 0.49 +0.5 316,999 - GFF3463 0.49 +0.2
Or see this region's nucleotide sequence