Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3425 and GFF3426 are separated by 29 nucleotides GFF3426 and GFF3427 are separated by 146 nucleotides
GFF3425 - putative FMNH2-dependent monooxygenase SfnC, at 271,515 to 272,756
GFF3425
GFF3426 - FAD-dependent urate hydroxylase, at 272,786 to 274,243
GFF3426
GFF3427 - Glutamine-dependent NAD(+) synthetase, at 274,390 to 276,435
GFF3427
Position (kb)
272
273
274
275 Strain fitness (log2 ratio)
-1
0
1 at 271.846 kb on - strand, within GFF3425 at 271.990 kb on - strand, within GFF3425 at 272.054 kb on - strand, within GFF3425 at 272.209 kb on - strand, within GFF3425 at 272.442 kb on + strand, within GFF3425 at 272.442 kb on + strand, within GFF3425 at 272.442 kb on + strand, within GFF3425 at 272.443 kb on - strand, within GFF3425 at 272.443 kb on - strand, within GFF3425 at 272.443 kb on - strand, within GFF3425 at 273.134 kb on + strand, within GFF3426 at 273.134 kb on + strand, within GFF3426 at 273.135 kb on - strand, within GFF3426 at 273.149 kb on + strand, within GFF3426 at 273.191 kb on + strand, within GFF3426 at 273.191 kb on + strand, within GFF3426 at 273.833 kb on + strand, within GFF3426 at 273.855 kb on - strand, within GFF3426 at 273.855 kb on - strand, within GFF3426 at 273.948 kb on - strand, within GFF3426 at 274.196 kb on + strand at 274.197 kb on - strand at 274.197 kb on - strand at 274.197 kb on - strand at 274.197 kb on - strand at 274.197 kb on - strand at 274.197 kb on - strand at 274.344 kb on + strand at 274.564 kb on + strand at 274.565 kb on - strand at 274.565 kb on - strand at 274.702 kb on + strand, within GFF3427 at 274.703 kb on - strand, within GFF3427 at 274.759 kb on + strand, within GFF3427 at 274.760 kb on - strand, within GFF3427 at 274.783 kb on + strand, within GFF3427 at 274.783 kb on + strand, within GFF3427 at 275.077 kb on + strand, within GFF3427 at 275.077 kb on + strand, within GFF3427 at 275.083 kb on + strand, within GFF3427 at 275.084 kb on - strand, within GFF3427
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 271,846 - GFF3425 0.27 -0.4 271,990 - GFF3425 0.38 -0.6 272,054 - GFF3425 0.43 +0.1 272,209 - GFF3425 0.56 -0.3 272,442 + GFF3425 0.75 -1.0 272,442 + GFF3425 0.75 -0.4 272,442 + GFF3425 0.75 -0.2 272,443 - GFF3425 0.75 +0.7 272,443 - GFF3425 0.75 +0.2 272,443 - GFF3425 0.75 -0.5 273,134 + GFF3426 0.24 -0.6 273,134 + GFF3426 0.24 +0.3 273,135 - GFF3426 0.24 -0.7 273,149 + GFF3426 0.25 +0.9 273,191 + GFF3426 0.28 +1.6 273,191 + GFF3426 0.28 -0.2 273,833 + GFF3426 0.72 -0.3 273,855 - GFF3426 0.73 -0.7 273,855 - GFF3426 0.73 -0.6 273,948 - GFF3426 0.80 -0.9 274,196 + +0.2 274,197 - -0.5 274,197 - -0.3 274,197 - -0.9 274,197 - +0.1 274,197 - +0.1 274,197 - +0.3 274,344 + -0.4 274,564 + +0.6 274,565 - -0.1 274,565 - -1.4 274,702 + GFF3427 0.15 +0.3 274,703 - GFF3427 0.15 -1.1 274,759 + GFF3427 0.18 +0.2 274,760 - GFF3427 0.18 -0.6 274,783 + GFF3427 0.19 -0.5 274,783 + GFF3427 0.19 +0.4 275,077 + GFF3427 0.34 -1.3 275,077 + GFF3427 0.34 +0.0 275,083 + GFF3427 0.34 -1.0 275,084 - GFF3427 0.34 -1.5
Or see this region's nucleotide sequence