Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3349 and GFF3350 overlap by 4 nucleotides GFF3350 and GFF3351 are separated by 3 nucleotides GFF3351 and GFF3352 are separated by 10 nucleotides GFF3352 and GFF3353 overlap by 1 nucleotides
GFF3349 - Lipopolysaccharide export system ATP-binding protein LptB, at 193,600 to 194,433
GFF3349
GFF3350 - Vitamin B12 import ATP-binding protein BtuD, at 194,430 to 195,188
GFF3350
GFF3351 - High-affinity branched-chain amino acid transport system permease protein LivH, at 195,192 to 196,055
GFF3351
GFF3352 - hypothetical protein, at 196,066 to 196,986
GFF3352
GFF3353 - hypothetical protein, at 196,986 to 197,567
GFF3353
Position (kb) 195
196
197 Strain fitness (log2 ratio) -2
-1
0
1 at 194.414 kb on - strand at 194.715 kb on + strand, within GFF3350 at 194.715 kb on + strand, within GFF3350 at 194.715 kb on + strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 194.716 kb on - strand, within GFF3350 at 195.327 kb on + strand, within GFF3351 at 195.337 kb on - strand, within GFF3351 at 195.843 kb on + strand, within GFF3351 at 196.075 kb on + strand at 196.075 kb on + strand at 196.075 kb on + strand at 196.075 kb on + strand at 196.076 kb on - strand at 196.076 kb on - strand at 196.076 kb on - strand at 196.199 kb on - strand, within GFF3352 at 196.318 kb on + strand, within GFF3352 at 196.681 kb on + strand, within GFF3352 at 196.681 kb on + strand, within GFF3352 at 196.681 kb on + strand, within GFF3352 at 196.681 kb on + strand, within GFF3352 at 196.681 kb on + strand, within GFF3352 at 196.682 kb on - strand, within GFF3352 at 196.682 kb on - strand, within GFF3352 at 196.682 kb on - strand, within GFF3352 at 196.683 kb on + strand, within GFF3352 at 196.684 kb on - strand, within GFF3352 at 196.721 kb on - strand, within GFF3352 at 196.721 kb on - strand, within GFF3352 at 196.721 kb on - strand, within GFF3352 at 196.721 kb on - strand, within GFF3352 at 196.721 kb on - strand, within GFF3352 at 196.727 kb on - strand, within GFF3352 at 196.901 kb on + strand at 196.901 kb on + strand at 196.901 kb on + strand at 196.902 kb on - strand at 197.031 kb on + strand at 197.031 kb on + strand at 197.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 194,414 - -0.2 194,715 + GFF3350 0.38 +0.8 194,715 + GFF3350 0.38 +1.1 194,715 + GFF3350 0.38 +0.5 194,716 - GFF3350 0.38 -0.2 194,716 - GFF3350 0.38 +0.0 194,716 - GFF3350 0.38 -0.0 194,716 - GFF3350 0.38 +0.2 194,716 - GFF3350 0.38 +1.6 194,716 - GFF3350 0.38 -0.0 195,327 + GFF3351 0.16 -0.5 195,337 - GFF3351 0.17 +0.2 195,843 + GFF3351 0.75 +0.9 196,075 + +0.1 196,075 + -0.1 196,075 + +0.0 196,075 + -0.9 196,076 - -0.5 196,076 - +0.2 196,076 - +1.3 196,199 - GFF3352 0.14 -0.0 196,318 + GFF3352 0.27 -0.5 196,681 + GFF3352 0.67 +0.1 196,681 + GFF3352 0.67 +0.4 196,681 + GFF3352 0.67 -0.0 196,681 + GFF3352 0.67 +0.2 196,681 + GFF3352 0.67 +0.7 196,682 - GFF3352 0.67 -0.6 196,682 - GFF3352 0.67 +0.6 196,682 - GFF3352 0.67 +0.5 196,683 + GFF3352 0.67 -0.7 196,684 - GFF3352 0.67 -0.1 196,721 - GFF3352 0.71 +0.4 196,721 - GFF3352 0.71 -0.6 196,721 - GFF3352 0.71 -0.2 196,721 - GFF3352 0.71 -0.1 196,721 - GFF3352 0.71 -2.0 196,727 - GFF3352 0.72 +0.4 196,901 + -1.0 196,901 + +0.9 196,901 + +1.1 196,902 - +0.1 197,031 + -0.1 197,031 + +0.7 197,032 - +0.6
Or see this region's nucleotide sequence