Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3336 and GFF3337 overlap by 4 nucleotides GFF3337 and GFF3338 are separated by 45 nucleotides
GFF3336 - Homoserine kinase, at 177,525 to 178,592
GFF3336
GFF3337 - Isoleucine 2-epimerase, at 178,589 to 179,935
GFF3337
GFF3338 - Beta-alanine--pyruvate aminotransferase, at 179,981 to 181,315
GFF3338
Position (kb)
178
179
180 Strain fitness (log2 ratio)
-1
0
1
2 at 177.718 kb on + strand, within GFF3336 at 177.718 kb on + strand, within GFF3336 at 177.719 kb on - strand, within GFF3336 at 177.719 kb on - strand, within GFF3336 at 177.719 kb on - strand, within GFF3336 at 177.719 kb on - strand, within GFF3336 at 177.864 kb on + strand, within GFF3336 at 177.864 kb on + strand, within GFF3336 at 178.207 kb on - strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.452 kb on + strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.453 kb on - strand, within GFF3336 at 178.718 kb on + strand at 178.730 kb on + strand, within GFF3337 at 178.730 kb on + strand, within GFF3337 at 178.730 kb on + strand, within GFF3337 at 178.730 kb on + strand, within GFF3337 at 178.730 kb on + strand, within GFF3337 at 178.731 kb on - strand, within GFF3337 at 178.797 kb on - strand, within GFF3337 at 178.797 kb on - strand, within GFF3337 at 178.808 kb on + strand, within GFF3337 at 178.863 kb on - strand, within GFF3337 at 178.863 kb on - strand, within GFF3337 at 178.899 kb on - strand, within GFF3337 at 178.899 kb on - strand, within GFF3337 at 178.899 kb on - strand, within GFF3337 at 179.030 kb on - strand, within GFF3337 at 179.030 kb on - strand, within GFF3337 at 179.067 kb on - strand, within GFF3337 at 179.067 kb on - strand, within GFF3337 at 179.067 kb on - strand, within GFF3337 at 179.067 kb on - strand, within GFF3337 at 179.216 kb on + strand, within GFF3337 at 179.216 kb on + strand, within GFF3337 at 179.216 kb on + strand, within GFF3337 at 179.217 kb on - strand, within GFF3337 at 179.217 kb on - strand, within GFF3337 at 179.297 kb on + strand, within GFF3337 at 179.297 kb on + strand, within GFF3337 at 179.297 kb on + strand, within GFF3337 at 179.297 kb on + strand, within GFF3337 at 179.298 kb on - strand, within GFF3337 at 179.540 kb on + strand, within GFF3337 at 179.541 kb on - strand, within GFF3337 at 179.720 kb on + strand, within GFF3337 at 179.721 kb on - strand, within GFF3337 at 179.721 kb on - strand, within GFF3337 at 179.721 kb on - strand, within GFF3337 at 179.721 kb on - strand, within GFF3337 at 179.722 kb on + strand, within GFF3337 at 179.722 kb on + strand, within GFF3337 at 179.722 kb on + strand, within GFF3337 at 179.835 kb on - strand at 179.932 kb on - strand at 180.129 kb on + strand, within GFF3338 at 180.129 kb on + strand, within GFF3338 at 180.130 kb on - strand, within GFF3338 at 180.130 kb on - strand, within GFF3338 at 180.200 kb on - strand, within GFF3338 at 180.200 kb on - strand, within GFF3338 at 180.294 kb on + strand, within GFF3338 at 180.294 kb on + strand, within GFF3338 at 180.294 kb on + strand, within GFF3338 at 180.295 kb on - strand, within GFF3338 at 180.295 kb on - strand, within GFF3338 at 180.295 kb on - strand, within GFF3338 at 180.295 kb on - strand, within GFF3338 at 180.295 kb on - strand, within GFF3338 at 180.345 kb on + strand, within GFF3338 at 180.345 kb on + strand, within GFF3338 at 180.345 kb on + strand, within GFF3338 at 180.345 kb on + strand, within GFF3338 at 180.345 kb on + strand, within GFF3338 at 180.345 kb on + strand, within GFF3338 at 180.346 kb on - strand, within GFF3338 at 180.346 kb on - strand, within GFF3338 at 180.346 kb on - strand, within GFF3338 at 180.346 kb on - strand, within GFF3338 at 180.346 kb on - strand, within GFF3338 at 180.349 kb on + strand, within GFF3338 at 180.349 kb on + strand, within GFF3338 at 180.349 kb on + strand, within GFF3338 at 180.349 kb on + strand, within GFF3338 at 180.350 kb on - strand, within GFF3338 at 180.350 kb on - strand, within GFF3338 at 180.350 kb on - strand, within GFF3338 at 180.351 kb on + strand, within GFF3338 at 180.352 kb on - strand, within GFF3338 at 180.473 kb on + strand, within GFF3338 at 180.474 kb on - strand, within GFF3338 at 180.474 kb on - strand, within GFF3338 at 180.474 kb on - strand, within GFF3338 at 180.474 kb on - strand, within GFF3338 at 180.474 kb on - strand, within GFF3338 at 180.495 kb on + strand, within GFF3338 at 180.496 kb on - strand, within GFF3338 at 180.496 kb on - strand, within GFF3338 at 180.496 kb on - strand, within GFF3338 at 180.600 kb on + strand, within GFF3338 at 180.600 kb on + strand, within GFF3338 at 180.601 kb on - strand, within GFF3338 at 180.834 kb on + strand, within GFF3338 at 180.861 kb on + strand, within GFF3338
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 177,718 + GFF3336 0.18 +0.6 177,718 + GFF3336 0.18 +1.3 177,719 - GFF3336 0.18 +0.2 177,719 - GFF3336 0.18 +1.0 177,719 - GFF3336 0.18 +0.4 177,719 - GFF3336 0.18 -0.6 177,864 + GFF3336 0.32 -0.4 177,864 + GFF3336 0.32 -0.9 178,207 - GFF3336 0.64 +0.3 178,452 + GFF3336 0.87 -0.4 178,452 + GFF3336 0.87 +0.1 178,452 + GFF3336 0.87 +0.0 178,452 + GFF3336 0.87 -0.4 178,452 + GFF3336 0.87 +0.5 178,452 + GFF3336 0.87 -0.3 178,452 + GFF3336 0.87 +0.4 178,453 - GFF3336 0.87 -0.2 178,453 - GFF3336 0.87 -1.2 178,453 - GFF3336 0.87 +0.6 178,453 - GFF3336 0.87 -0.2 178,453 - GFF3336 0.87 +0.7 178,453 - GFF3336 0.87 -0.4 178,453 - GFF3336 0.87 -0.2 178,453 - GFF3336 0.87 -0.3 178,718 + +0.4 178,730 + GFF3337 0.10 +0.2 178,730 + GFF3337 0.10 -0.1 178,730 + GFF3337 0.10 +0.6 178,730 + GFF3337 0.10 -0.1 178,730 + GFF3337 0.10 -1.1 178,731 - GFF3337 0.11 -0.1 178,797 - GFF3337 0.15 +0.1 178,797 - GFF3337 0.15 +0.5 178,808 + GFF3337 0.16 +1.0 178,863 - GFF3337 0.20 +1.9 178,863 - GFF3337 0.20 +0.3 178,899 - GFF3337 0.23 -0.2 178,899 - GFF3337 0.23 +0.0 178,899 - GFF3337 0.23 +0.6 179,030 - GFF3337 0.33 -0.2 179,030 - GFF3337 0.33 +0.1 179,067 - GFF3337 0.35 +0.1 179,067 - GFF3337 0.35 +0.5 179,067 - GFF3337 0.35 +0.1 179,067 - GFF3337 0.35 -0.7 179,216 + GFF3337 0.47 +0.5 179,216 + GFF3337 0.47 +0.4 179,216 + GFF3337 0.47 +0.7 179,217 - GFF3337 0.47 -0.1 179,217 - GFF3337 0.47 +0.1 179,297 + GFF3337 0.53 -0.3 179,297 + GFF3337 0.53 +0.5 179,297 + GFF3337 0.53 -0.3 179,297 + GFF3337 0.53 +0.5 179,298 - GFF3337 0.53 -0.1 179,540 + GFF3337 0.71 -0.7 179,541 - GFF3337 0.71 +1.1 179,720 + GFF3337 0.84 -0.4 179,721 - GFF3337 0.84 -0.2 179,721 - GFF3337 0.84 -1.4 179,721 - GFF3337 0.84 -0.5 179,721 - GFF3337 0.84 -1.5 179,722 + GFF3337 0.84 +0.2 179,722 + GFF3337 0.84 -0.9 179,722 + GFF3337 0.84 -0.8 179,835 - +0.3 179,932 - +0.7 180,129 + GFF3338 0.11 -0.3 180,129 + GFF3338 0.11 +0.2 180,130 - GFF3338 0.11 +0.5 180,130 - GFF3338 0.11 -0.0 180,200 - GFF3338 0.16 +0.0 180,200 - GFF3338 0.16 -0.1 180,294 + GFF3338 0.23 +0.8 180,294 + GFF3338 0.23 -1.1 180,294 + GFF3338 0.23 -0.4 180,295 - GFF3338 0.24 +0.2 180,295 - GFF3338 0.24 -0.2 180,295 - GFF3338 0.24 +0.2 180,295 - GFF3338 0.24 +0.2 180,295 - GFF3338 0.24 -0.1 180,345 + GFF3338 0.27 -1.0 180,345 + GFF3338 0.27 -0.3 180,345 + GFF3338 0.27 -0.2 180,345 + GFF3338 0.27 -1.0 180,345 + GFF3338 0.27 -0.4 180,345 + GFF3338 0.27 +1.0 180,346 - GFF3338 0.27 -0.8 180,346 - GFF3338 0.27 -0.1 180,346 - GFF3338 0.27 +0.8 180,346 - GFF3338 0.27 +0.5 180,346 - GFF3338 0.27 -0.0 180,349 + GFF3338 0.28 +0.2 180,349 + GFF3338 0.28 +0.3 180,349 + GFF3338 0.28 +0.2 180,349 + GFF3338 0.28 -0.2 180,350 - GFF3338 0.28 +0.2 180,350 - GFF3338 0.28 -0.0 180,350 - GFF3338 0.28 +0.4 180,351 + GFF3338 0.28 -0.8 180,352 - GFF3338 0.28 -0.7 180,473 + GFF3338 0.37 -1.1 180,474 - GFF3338 0.37 +0.8 180,474 - GFF3338 0.37 -0.6 180,474 - GFF3338 0.37 -0.9 180,474 - GFF3338 0.37 +0.2 180,474 - GFF3338 0.37 +1.3 180,495 + GFF3338 0.39 +0.2 180,496 - GFF3338 0.39 +1.3 180,496 - GFF3338 0.39 -0.3 180,496 - GFF3338 0.39 +0.8 180,600 + GFF3338 0.46 -0.3 180,600 + GFF3338 0.46 +0.1 180,601 - GFF3338 0.46 -0.3 180,834 + GFF3338 0.64 -0.4 180,861 + GFF3338 0.66 +0.5
Or see this region's nucleotide sequence