Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3306 and GFF3307 are separated by 71 nucleotides GFF3307 and GFF3308 are separated by 85 nucleotides GFF3308 and GFF3309 are separated by 85 nucleotides
GFF3306 - D-galactarolactone cycloisomerase, at 144,088 to 145,230
GFF3306
GFF3307 - hypothetical protein, at 145,302 to 146,303
GFF3307
GFF3308 - hypothetical protein, at 146,389 to 147,102
GFF3308
GFF3309 - hypothetical protein, at 147,188 to 148,417
GFF3309
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2 at 144.528 kb on - strand, within GFF3306 at 144.654 kb on + strand, within GFF3306 at 144.686 kb on - strand, within GFF3306 at 144.695 kb on + strand, within GFF3306 at 144.695 kb on + strand, within GFF3306 at 144.695 kb on + strand, within GFF3306 at 144.695 kb on + strand, within GFF3306 at 144.696 kb on - strand, within GFF3306 at 144.758 kb on + strand, within GFF3306 at 144.812 kb on + strand, within GFF3306 at 144.827 kb on + strand, within GFF3306 at 144.898 kb on - strand, within GFF3306 at 144.977 kb on + strand, within GFF3306 at 144.978 kb on - strand, within GFF3306 at 145.079 kb on + strand, within GFF3306 at 145.148 kb on + strand at 145.148 kb on + strand at 145.516 kb on + strand, within GFF3307 at 145.516 kb on + strand, within GFF3307 at 145.517 kb on - strand, within GFF3307 at 145.555 kb on + strand, within GFF3307 at 145.674 kb on + strand, within GFF3307 at 145.675 kb on - strand, within GFF3307 at 145.747 kb on + strand, within GFF3307 at 145.747 kb on + strand, within GFF3307 at 145.801 kb on + strand, within GFF3307 at 145.801 kb on + strand, within GFF3307 at 145.802 kb on - strand, within GFF3307 at 145.802 kb on - strand, within GFF3307 at 146.011 kb on + strand, within GFF3307 at 146.011 kb on + strand, within GFF3307 at 146.011 kb on + strand, within GFF3307 at 146.346 kb on + strand at 146.346 kb on + strand at 146.346 kb on + strand at 146.347 kb on - strand at 146.803 kb on + strand, within GFF3308 at 146.803 kb on + strand, within GFF3308 at 146.839 kb on + strand, within GFF3308 at 146.839 kb on + strand, within GFF3308 at 146.840 kb on - strand, within GFF3308 at 146.840 kb on - strand, within GFF3308 at 146.840 kb on - strand, within GFF3308 at 146.840 kb on - strand, within GFF3308 at 146.840 kb on - strand, within GFF3308 at 146.929 kb on + strand, within GFF3308 at 146.929 kb on + strand, within GFF3308 at 146.929 kb on + strand, within GFF3308 at 146.930 kb on - strand, within GFF3308 at 147.047 kb on - strand at 147.268 kb on - strand at 147.268 kb on - strand at 147.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 144,528 - GFF3306 0.38 +0.4 144,654 + GFF3306 0.50 -0.2 144,686 - GFF3306 0.52 -0.6 144,695 + GFF3306 0.53 -0.2 144,695 + GFF3306 0.53 -0.1 144,695 + GFF3306 0.53 +0.5 144,695 + GFF3306 0.53 +0.2 144,696 - GFF3306 0.53 -0.2 144,758 + GFF3306 0.59 -0.2 144,812 + GFF3306 0.63 +0.1 144,827 + GFF3306 0.65 -0.8 144,898 - GFF3306 0.71 -0.4 144,977 + GFF3306 0.78 -1.0 144,978 - GFF3306 0.78 -0.3 145,079 + GFF3306 0.87 -0.1 145,148 + -0.1 145,148 + -1.4 145,516 + GFF3307 0.21 -0.6 145,516 + GFF3307 0.21 +0.8 145,517 - GFF3307 0.21 +0.5 145,555 + GFF3307 0.25 -0.0 145,674 + GFF3307 0.37 -0.0 145,675 - GFF3307 0.37 -0.7 145,747 + GFF3307 0.44 -0.1 145,747 + GFF3307 0.44 -0.2 145,801 + GFF3307 0.50 +1.4 145,801 + GFF3307 0.50 -0.3 145,802 - GFF3307 0.50 -0.3 145,802 - GFF3307 0.50 +1.1 146,011 + GFF3307 0.71 -0.3 146,011 + GFF3307 0.71 +0.5 146,011 + GFF3307 0.71 +0.0 146,346 + -2.4 146,346 + -0.1 146,346 + +0.6 146,347 - -0.3 146,803 + GFF3308 0.58 -0.3 146,803 + GFF3308 0.58 +1.4 146,839 + GFF3308 0.63 -0.0 146,839 + GFF3308 0.63 +0.6 146,840 - GFF3308 0.63 +2.2 146,840 - GFF3308 0.63 +0.4 146,840 - GFF3308 0.63 +1.0 146,840 - GFF3308 0.63 -0.8 146,840 - GFF3308 0.63 -0.2 146,929 + GFF3308 0.76 -0.2 146,929 + GFF3308 0.76 -0.8 146,929 + GFF3308 0.76 -0.4 146,930 - GFF3308 0.76 -1.3 147,047 - +0.1 147,268 - +1.1 147,268 - -0.4 147,268 - +0.4
Or see this region's nucleotide sequence