Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3249 and GFF3250 are separated by 311 nucleotides GFF3250 and GFF3251 are separated by 120 nucleotides
GFF3249 - High-affinity branched-chain amino acid transport system permease protein LivH, at 78,593 to 79,576
GFF3249
GFF3250 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 79,888 to 81,060
GFF3250
GFF3251 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 81,181 to 82,662
GFF3251
Position (kb) 79
80
81
82 Strain fitness (log2 ratio) -2
-1
0
1 at 79.038 kb on + strand, within GFF3249 at 79.038 kb on + strand, within GFF3249 at 79.038 kb on + strand, within GFF3249 at 79.039 kb on - strand, within GFF3249 at 79.086 kb on + strand, within GFF3249 at 79.086 kb on + strand, within GFF3249 at 79.086 kb on + strand, within GFF3249 at 79.095 kb on + strand, within GFF3249 at 79.096 kb on - strand, within GFF3249 at 79.248 kb on + strand, within GFF3249 at 79.380 kb on + strand, within GFF3249 at 79.380 kb on + strand, within GFF3249 at 79.381 kb on - strand, within GFF3249 at 79.381 kb on - strand, within GFF3249 at 79.746 kb on + strand at 79.925 kb on + strand at 79.925 kb on + strand at 79.997 kb on + strand at 79.997 kb on + strand at 79.998 kb on - strand at 80.153 kb on + strand, within GFF3250 at 80.168 kb on + strand, within GFF3250 at 80.183 kb on + strand, within GFF3250 at 80.183 kb on + strand, within GFF3250 at 80.183 kb on + strand, within GFF3250 at 80.183 kb on + strand, within GFF3250 at 80.184 kb on - strand, within GFF3250 at 80.189 kb on + strand, within GFF3250 at 80.189 kb on + strand, within GFF3250 at 80.189 kb on + strand, within GFF3250 at 80.189 kb on + strand, within GFF3250 at 80.364 kb on - strand, within GFF3250 at 80.480 kb on + strand, within GFF3250 at 80.480 kb on + strand, within GFF3250 at 80.480 kb on + strand, within GFF3250 at 80.481 kb on - strand, within GFF3250 at 80.508 kb on - strand, within GFF3250 at 80.510 kb on + strand, within GFF3250 at 80.549 kb on + strand, within GFF3250 at 80.597 kb on + strand, within GFF3250 at 80.597 kb on + strand, within GFF3250 at 80.634 kb on - strand, within GFF3250 at 81.230 kb on + strand at 81.294 kb on - strand at 81.363 kb on - strand, within GFF3251 at 81.410 kb on + strand, within GFF3251 at 81.410 kb on + strand, within GFF3251 at 81.410 kb on + strand, within GFF3251 at 81.410 kb on + strand, within GFF3251 at 81.410 kb on + strand, within GFF3251 at 81.411 kb on - strand, within GFF3251 at 81.426 kb on - strand, within GFF3251 at 81.840 kb on - strand, within GFF3251
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 79,038 + GFF3249 0.45 +0.6 79,038 + GFF3249 0.45 +0.4 79,038 + GFF3249 0.45 +0.1 79,039 - GFF3249 0.45 -0.3 79,086 + GFF3249 0.50 +1.1 79,086 + GFF3249 0.50 -1.1 79,086 + GFF3249 0.50 +0.5 79,095 + GFF3249 0.51 -0.3 79,096 - GFF3249 0.51 -0.3 79,248 + GFF3249 0.67 -0.2 79,380 + GFF3249 0.80 +0.1 79,380 + GFF3249 0.80 +0.2 79,381 - GFF3249 0.80 +0.1 79,381 - GFF3249 0.80 +0.1 79,746 + +0.9 79,925 + -1.0 79,925 + +0.3 79,997 + +0.2 79,997 + -0.7 79,998 - +0.2 80,153 + GFF3250 0.23 +0.2 80,168 + GFF3250 0.24 -0.2 80,183 + GFF3250 0.25 +0.6 80,183 + GFF3250 0.25 -0.1 80,183 + GFF3250 0.25 +0.7 80,183 + GFF3250 0.25 +0.9 80,184 - GFF3250 0.25 -0.7 80,189 + GFF3250 0.26 +0.8 80,189 + GFF3250 0.26 -0.3 80,189 + GFF3250 0.26 -0.0 80,189 + GFF3250 0.26 -0.3 80,364 - GFF3250 0.41 -0.3 80,480 + GFF3250 0.50 -1.6 80,480 + GFF3250 0.50 -0.4 80,480 + GFF3250 0.50 -0.9 80,481 - GFF3250 0.51 +0.2 80,508 - GFF3250 0.53 -0.0 80,510 + GFF3250 0.53 -0.9 80,549 + GFF3250 0.56 -1.2 80,597 + GFF3250 0.60 -0.5 80,597 + GFF3250 0.60 +0.7 80,634 - GFF3250 0.64 -0.7 81,230 + -0.7 81,294 - -0.2 81,363 - GFF3251 0.12 -0.3 81,410 + GFF3251 0.15 +0.5 81,410 + GFF3251 0.15 +0.9 81,410 + GFF3251 0.15 -2.0 81,410 + GFF3251 0.15 +0.2 81,410 + GFF3251 0.15 +1.2 81,411 - GFF3251 0.16 +0.1 81,426 - GFF3251 0.17 +0.3 81,840 - GFF3251 0.44 +0.6
Or see this region's nucleotide sequence