Strain Fitness in Xanthobacter sp. DMC5 around GFF3224

Experiment: Methanol and casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3223 and GFF3224 are separated by 31 nucleotidesGFF3224 and GFF3225 are separated by 21 nucleotides GFF3223 - 3-oxoacyl-[acyl-carrier-protein] synthase 2, at 49,371 to 50,654 GFF3223 GFF3224 - 3-oxoacyl-[acyl-carrier-protein] reductase FabG, at 50,686 to 51,438 GFF3224 GFF3225 - Alcohol dehydrogenase, at 51,460 to 52,482 GFF3225 Position (kb) 50 51 52Strain fitness (log2 ratio) -1 0 1at 50.764 kb on + strand, within GFF3224at 50.782 kb on + strand, within GFF3224at 50.968 kb on - strand, within GFF3224at 50.968 kb on - strand, within GFF3224at 51.142 kb on + strand, within GFF3224at 51.142 kb on + strand, within GFF3224at 51.143 kb on - strand, within GFF3224at 51.224 kb on - strand, within GFF3224at 51.224 kb on - strand, within GFF3224at 51.224 kb on - strand, within GFF3224at 51.375 kb on + strandat 51.375 kb on + strandat 51.375 kb on + strandat 51.447 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol and casaminos (C)
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50,764 + GFF3224 0.10 +0.5
50,782 + GFF3224 0.13 +0.2
50,968 - GFF3224 0.37 +0.6
50,968 - GFF3224 0.37 +0.9
51,142 + GFF3224 0.61 -0.1
51,142 + GFF3224 0.61 -0.2
51,143 - GFF3224 0.61 +0.3
51,224 - GFF3224 0.71 +1.3
51,224 - GFF3224 0.71 -0.0
51,224 - GFF3224 0.71 +0.4
51,375 + -1.3
51,375 + -0.3
51,375 + +0.6
51,447 + -0.6

Or see this region's nucleotide sequence