Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3100 and GFF3101 are separated by 365 nucleotides GFF3101 and GFF3102 are separated by 10 nucleotides GFF3102 and GFF3103 are separated by 165 nucleotides GFF3103 and GFF3104 are separated by 20 nucleotides
GFF3100 - GTP cyclohydrolase 1, at 829,488 to 830,213
GFF3100
GFF3101 - hypothetical protein, at 830,579 to 831,022
GFF3101
GFF3102 - hypothetical protein, at 831,033 to 831,632
GFF3102
GFF3103 - Putative membrane protein insertion efficiency factor, at 831,798 to 832,166
GFF3103
GFF3104 - Biosynthetic peptidoglycan transglycosylase, at 832,187 to 834,460
GFF3104
Position (kb) 831
832 Strain fitness (log2 ratio) -1
0
1 at 830.514 kb on - strand at 831.169 kb on + strand, within GFF3102 at 831.169 kb on + strand, within GFF3102 at 831.169 kb on + strand, within GFF3102 at 831.170 kb on - strand, within GFF3102 at 831.477 kb on + strand, within GFF3102 at 831.477 kb on + strand, within GFF3102 at 831.512 kb on - strand, within GFF3102 at 831.798 kb on - strand at 831.913 kb on - strand, within GFF3103 at 832.066 kb on - strand, within GFF3103 at 832.108 kb on - strand, within GFF3103 at 832.491 kb on + strand, within GFF3104 at 832.491 kb on + strand, within GFF3104
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 830,514 - +0.5 831,169 + GFF3102 0.23 -1.0 831,169 + GFF3102 0.23 -1.0 831,169 + GFF3102 0.23 +0.5 831,170 - GFF3102 0.23 -0.4 831,477 + GFF3102 0.74 +0.4 831,477 + GFF3102 0.74 +0.2 831,512 - GFF3102 0.80 +0.6 831,798 - +0.6 831,913 - GFF3103 0.31 +0.8 832,066 - GFF3103 0.73 -0.3 832,108 - GFF3103 0.84 +0.9 832,491 + GFF3104 0.13 -0.5 832,491 + GFF3104 0.13 +0.5
Or see this region's nucleotide sequence