Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3001 and GFF3002 overlap by 37 nucleotides GFF3002 and GFF3003 overlap by 4 nucleotides GFF3003 and GFF3004 are separated by 7 nucleotides GFF3004 and GFF3005 are separated by 113 nucleotides
GFF3001 - putative MFS-type transporter, at 729,144 to 730,469
GFF3001
GFF3002 - hypothetical protein, at 730,433 to 730,849
GFF3002
GFF3003 - hypothetical protein, at 730,846 to 731,253
GFF3003
GFF3004 - hypothetical protein, at 731,261 to 731,734
GFF3004
GFF3005 - Glycine cleavage system transcriptional activator, at 731,848 to 732,750
GFF3005
Position (kb) 730
731
732 Strain fitness (log2 ratio) -1
0
1 at 729.881 kb on + strand, within GFF3001 at 729.945 kb on + strand, within GFF3001 at 729.945 kb on + strand, within GFF3001 at 729.945 kb on + strand, within GFF3001 at 729.945 kb on + strand, within GFF3001 at 729.946 kb on - strand, within GFF3001 at 729.946 kb on - strand, within GFF3001 at 729.946 kb on - strand, within GFF3001 at 730.113 kb on + strand, within GFF3001 at 730.114 kb on - strand, within GFF3001 at 730.114 kb on - strand, within GFF3001 at 730.383 kb on + strand at 730.384 kb on - strand at 730.384 kb on - strand at 730.467 kb on + strand at 730.468 kb on - strand at 730.647 kb on + strand, within GFF3002 at 730.647 kb on + strand, within GFF3002 at 730.648 kb on - strand, within GFF3002 at 730.648 kb on - strand, within GFF3002 at 730.742 kb on + strand, within GFF3002 at 730.743 kb on - strand, within GFF3002 at 730.926 kb on - strand, within GFF3003 at 730.929 kb on - strand, within GFF3003 at 730.929 kb on - strand, within GFF3003 at 730.940 kb on + strand, within GFF3003 at 731.043 kb on - strand, within GFF3003 at 731.338 kb on - strand, within GFF3004 at 731.379 kb on + strand, within GFF3004 at 731.482 kb on + strand, within GFF3004 at 731.509 kb on + strand, within GFF3004 at 731.511 kb on + strand, within GFF3004 at 731.511 kb on + strand, within GFF3004 at 731.511 kb on + strand, within GFF3004 at 731.512 kb on - strand, within GFF3004 at 731.512 kb on - strand, within GFF3004 at 731.512 kb on - strand, within GFF3004 at 731.512 kb on - strand, within GFF3004 at 731.667 kb on + strand, within GFF3004 at 731.668 kb on - strand, within GFF3004 at 731.668 kb on - strand, within GFF3004 at 731.783 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 729,881 + GFF3001 0.56 -0.6 729,945 + GFF3001 0.60 -0.3 729,945 + GFF3001 0.60 +0.8 729,945 + GFF3001 0.60 -0.8 729,945 + GFF3001 0.60 -0.1 729,946 - GFF3001 0.60 +0.8 729,946 - GFF3001 0.60 +0.2 729,946 - GFF3001 0.60 +0.9 730,113 + GFF3001 0.73 -1.6 730,114 - GFF3001 0.73 +0.2 730,114 - GFF3001 0.73 +0.1 730,383 + -0.3 730,384 - -0.1 730,384 - -0.3 730,467 + -0.3 730,468 - -0.7 730,647 + GFF3002 0.51 +0.2 730,647 + GFF3002 0.51 +0.5 730,648 - GFF3002 0.52 -0.1 730,648 - GFF3002 0.52 -0.6 730,742 + GFF3002 0.74 +0.3 730,743 - GFF3002 0.74 +0.1 730,926 - GFF3003 0.20 +0.3 730,929 - GFF3003 0.20 +0.6 730,929 - GFF3003 0.20 -0.1 730,940 + GFF3003 0.23 -0.2 731,043 - GFF3003 0.48 -0.5 731,338 - GFF3004 0.16 -0.4 731,379 + GFF3004 0.25 -1.1 731,482 + GFF3004 0.47 -0.7 731,509 + GFF3004 0.52 -0.0 731,511 + GFF3004 0.53 -0.5 731,511 + GFF3004 0.53 +0.1 731,511 + GFF3004 0.53 -0.4 731,512 - GFF3004 0.53 +0.3 731,512 - GFF3004 0.53 +0.9 731,512 - GFF3004 0.53 -0.9 731,512 - GFF3004 0.53 +0.2 731,667 + GFF3004 0.86 +0.4 731,668 - GFF3004 0.86 +0.2 731,668 - GFF3004 0.86 -0.5 731,783 - -1.4
Or see this region's nucleotide sequence