Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF29 and GFF30 are separated by 13 nucleotides GFF30 and GFF31 are separated by 37 nucleotides
GFF29 - 2,3-diketo-L-gulonate-binding periplasmic protein YiaO, at 33,943 to 34,971
GFF29
GFF30 - Sialic acid TRAP transporter permease protein SiaT, at 34,985 to 36,856
GFF30
GFF31 - Long-chain-fatty-acid--CoA ligase, at 36,894 to 38,456
GFF31
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 34.312 kb on + strand, within GFF29 at 34.312 kb on + strand, within GFF29 at 34.312 kb on + strand, within GFF29 at 34.313 kb on - strand, within GFF29 at 34.565 kb on - strand, within GFF29 at 34.621 kb on + strand, within GFF29 at 34.621 kb on + strand, within GFF29 at 34.622 kb on - strand, within GFF29 at 34.622 kb on - strand, within GFF29 at 34.652 kb on - strand, within GFF29 at 35.035 kb on - strand at 35.141 kb on + strand at 35.141 kb on + strand at 35.142 kb on - strand at 35.142 kb on - strand at 35.245 kb on + strand, within GFF30 at 35.245 kb on + strand, within GFF30 at 35.245 kb on + strand, within GFF30 at 35.246 kb on - strand, within GFF30 at 35.356 kb on + strand, within GFF30 at 35.357 kb on + strand, within GFF30 at 35.358 kb on - strand, within GFF30 at 35.452 kb on + strand, within GFF30 at 35.452 kb on + strand, within GFF30 at 35.679 kb on - strand, within GFF30 at 35.725 kb on + strand, within GFF30 at 35.726 kb on - strand, within GFF30 at 35.726 kb on - strand, within GFF30 at 35.870 kb on + strand, within GFF30 at 35.870 kb on + strand, within GFF30 at 35.871 kb on - strand, within GFF30 at 35.871 kb on - strand, within GFF30 at 35.871 kb on - strand, within GFF30 at 35.874 kb on + strand, within GFF30 at 35.874 kb on + strand, within GFF30 at 35.874 kb on + strand, within GFF30 at 35.875 kb on - strand, within GFF30 at 35.891 kb on + strand, within GFF30 at 35.891 kb on + strand, within GFF30 at 35.891 kb on + strand, within GFF30 at 35.892 kb on - strand, within GFF30 at 35.892 kb on - strand, within GFF30 at 35.892 kb on - strand, within GFF30 at 35.892 kb on - strand, within GFF30 at 36.327 kb on - strand, within GFF30 at 36.380 kb on + strand, within GFF30 at 36.380 kb on + strand, within GFF30 at 36.381 kb on - strand, within GFF30 at 36.397 kb on + strand, within GFF30 at 36.657 kb on - strand, within GFF30 at 36.657 kb on - strand, within GFF30 at 36.657 kb on - strand, within GFF30 at 36.719 kb on + strand at 36.719 kb on + strand at 36.720 kb on - strand at 36.720 kb on - strand at 36.722 kb on + strand at 36.723 kb on - strand at 36.782 kb on + strand at 37.080 kb on + strand, within GFF31 at 37.081 kb on - strand, within GFF31 at 37.108 kb on - strand, within GFF31 at 37.108 kb on - strand, within GFF31 at 37.272 kb on + strand, within GFF31 at 37.405 kb on - strand, within GFF31 at 37.405 kb on - strand, within GFF31 at 37.405 kb on - strand, within GFF31 at 37.405 kb on - strand, within GFF31 at 37.458 kb on - strand, within GFF31 at 37.475 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.532 kb on + strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.533 kb on - strand, within GFF31 at 37.576 kb on - strand, within GFF31 at 37.576 kb on - strand, within GFF31 at 37.600 kb on - strand, within GFF31 at 37.686 kb on + strand, within GFF31 at 37.686 kb on + strand, within GFF31 at 37.686 kb on + strand, within GFF31 at 37.687 kb on - strand, within GFF31 at 37.729 kb on - strand, within GFF31
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 34,312 + GFF29 0.36 +0.8 34,312 + GFF29 0.36 -0.4 34,312 + GFF29 0.36 +0.5 34,313 - GFF29 0.36 +0.7 34,565 - GFF29 0.60 +0.0 34,621 + GFF29 0.66 -0.4 34,621 + GFF29 0.66 +0.3 34,622 - GFF29 0.66 -0.8 34,622 - GFF29 0.66 -0.4 34,652 - GFF29 0.69 +0.2 35,035 - +1.2 35,141 + -0.8 35,141 + +0.1 35,142 - -1.7 35,142 - -0.8 35,245 + GFF30 0.14 -0.8 35,245 + GFF30 0.14 +0.2 35,245 + GFF30 0.14 -1.0 35,246 - GFF30 0.14 -0.1 35,356 + GFF30 0.20 +0.8 35,357 + GFF30 0.20 -0.7 35,358 - GFF30 0.20 +0.7 35,452 + GFF30 0.25 +0.6 35,452 + GFF30 0.25 -0.4 35,679 - GFF30 0.37 -0.7 35,725 + GFF30 0.40 +0.2 35,726 - GFF30 0.40 -1.1 35,726 - GFF30 0.40 -0.5 35,870 + GFF30 0.47 +0.1 35,870 + GFF30 0.47 -1.5 35,871 - GFF30 0.47 +0.1 35,871 - GFF30 0.47 +0.8 35,871 - GFF30 0.47 -0.4 35,874 + GFF30 0.47 +0.8 35,874 + GFF30 0.47 +0.8 35,874 + GFF30 0.47 +0.9 35,875 - GFF30 0.48 -0.2 35,891 + GFF30 0.48 +0.6 35,891 + GFF30 0.48 -0.7 35,891 + GFF30 0.48 -0.7 35,892 - GFF30 0.48 +1.1 35,892 - GFF30 0.48 -0.2 35,892 - GFF30 0.48 -0.3 35,892 - GFF30 0.48 +0.5 36,327 - GFF30 0.72 +1.2 36,380 + GFF30 0.75 -0.4 36,380 + GFF30 0.75 +0.6 36,381 - GFF30 0.75 -0.0 36,397 + GFF30 0.75 +0.7 36,657 - GFF30 0.89 +0.1 36,657 - GFF30 0.89 -0.6 36,657 - GFF30 0.89 -0.1 36,719 + -0.5 36,719 + +0.6 36,720 - -0.2 36,720 - +1.0 36,722 + -0.2 36,723 - +0.2 36,782 + -2.9 37,080 + GFF31 0.12 +0.8 37,081 - GFF31 0.12 +0.0 37,108 - GFF31 0.14 +0.4 37,108 - GFF31 0.14 +0.2 37,272 + GFF31 0.24 -0.6 37,405 - GFF31 0.33 -1.1 37,405 - GFF31 0.33 +0.6 37,405 - GFF31 0.33 -0.1 37,405 - GFF31 0.33 +0.7 37,458 - GFF31 0.36 -0.3 37,475 + GFF31 0.37 -0.5 37,532 + GFF31 0.41 -1.2 37,532 + GFF31 0.41 +0.1 37,532 + GFF31 0.41 -0.5 37,532 + GFF31 0.41 -0.5 37,532 + GFF31 0.41 -0.3 37,532 + GFF31 0.41 -0.9 37,532 + GFF31 0.41 +0.2 37,533 - GFF31 0.41 +0.5 37,533 - GFF31 0.41 +0.7 37,533 - GFF31 0.41 -0.5 37,533 - GFF31 0.41 +0.8 37,533 - GFF31 0.41 -0.1 37,533 - GFF31 0.41 -0.4 37,533 - GFF31 0.41 +0.4 37,533 - GFF31 0.41 -0.3 37,533 - GFF31 0.41 +0.7 37,576 - GFF31 0.44 -0.0 37,576 - GFF31 0.44 +0.0 37,600 - GFF31 0.45 -0.3 37,686 + GFF31 0.51 -0.6 37,686 + GFF31 0.51 +0.5 37,686 + GFF31 0.51 -0.1 37,687 - GFF31 0.51 +0.3 37,729 - GFF31 0.53 +0.3
Or see this region's nucleotide sequence