Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF295 and GFF296 are separated by 538 nucleotides GFF296 and GFF297 overlap by 4 nucleotides GFF297 and GFF298 overlap by 14 nucleotides GFF298 and GFF299 are separated by 9 nucleotides GFF299 and GFF300 are separated by 3 nucleotides
GFF295 - hypothetical protein, at 310,434 to 311,120
GFF295
GFF296 - hypothetical protein, at 311,659 to 312,783
GFF296
GFF297 - hypothetical protein, at 312,780 to 313,031
GFF297
GFF298 - hypothetical protein, at 313,018 to 313,233
GFF298
GFF299 - Single-stranded DNA-binding protein, at 313,243 to 313,668
GFF299
GFF300 - ATP-dependent zinc metalloprotease FtsH, at 313,672 to 314,595
GFF300
Position (kb)
311
312
313 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 310.669 kb on + strand, within GFF295 at 310.669 kb on + strand, within GFF295 at 310.717 kb on + strand, within GFF295 at 310.717 kb on + strand, within GFF295 at 310.717 kb on + strand, within GFF295 at 310.876 kb on - strand, within GFF295 at 310.876 kb on - strand, within GFF295 at 310.876 kb on - strand, within GFF295 at 311.041 kb on + strand, within GFF295 at 311.042 kb on - strand, within GFF295 at 311.434 kb on + strand at 311.635 kb on + strand at 311.762 kb on + strand at 311.762 kb on + strand at 311.763 kb on - strand at 311.763 kb on - strand at 311.962 kb on + strand, within GFF296 at 311.962 kb on + strand, within GFF296 at 311.962 kb on + strand, within GFF296 at 311.962 kb on + strand, within GFF296 at 311.963 kb on - strand, within GFF296 at 312.097 kb on + strand, within GFF296 at 312.105 kb on + strand, within GFF296 at 312.107 kb on + strand, within GFF296 at 312.107 kb on + strand, within GFF296 at 312.107 kb on + strand, within GFF296 at 312.107 kb on + strand, within GFF296 at 312.107 kb on + strand, within GFF296 at 312.164 kb on + strand, within GFF296 at 312.164 kb on + strand, within GFF296 at 312.164 kb on + strand, within GFF296 at 312.164 kb on + strand, within GFF296 at 312.165 kb on - strand, within GFF296 at 312.165 kb on - strand, within GFF296 at 312.165 kb on - strand, within GFF296 at 312.217 kb on - strand, within GFF296 at 312.398 kb on + strand, within GFF296 at 312.398 kb on + strand, within GFF296 at 312.398 kb on + strand, within GFF296 at 312.629 kb on + strand, within GFF296 at 312.629 kb on + strand, within GFF296 at 312.630 kb on - strand, within GFF296 at 312.630 kb on - strand, within GFF296 at 312.704 kb on + strand at 312.704 kb on + strand at 312.705 kb on - strand at 312.705 kb on - strand at 312.705 kb on - strand at 312.705 kb on - strand at 312.705 kb on - strand at 312.705 kb on - strand at 312.705 kb on - strand at 312.740 kb on + strand at 312.741 kb on - strand at 312.741 kb on - strand at 312.741 kb on - strand at 312.746 kb on + strand at 312.746 kb on + strand at 312.746 kb on + strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.747 kb on - strand at 312.784 kb on - strand at 312.936 kb on + strand, within GFF297 at 312.960 kb on - strand, within GFF297 at 312.961 kb on + strand, within GFF297 at 312.961 kb on + strand, within GFF297 at 312.961 kb on + strand, within GFF297 at 313.194 kb on - strand, within GFF298 at 313.194 kb on - strand, within GFF298 at 313.289 kb on + strand, within GFF299 at 313.379 kb on + strand, within GFF299 at 313.380 kb on - strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.387 kb on + strand, within GFF299 at 313.388 kb on - strand, within GFF299 at 313.388 kb on - strand, within GFF299 at 313.388 kb on - strand, within GFF299 at 313.436 kb on + strand, within GFF299 at 313.491 kb on + strand, within GFF299 at 313.493 kb on + strand, within GFF299 at 313.493 kb on + strand, within GFF299 at 313.493 kb on + strand, within GFF299 at 313.493 kb on + strand, within GFF299 at 313.494 kb on - strand, within GFF299 at 313.494 kb on - strand, within GFF299 at 313.494 kb on - strand, within GFF299 at 313.494 kb on - strand, within GFF299 at 313.634 kb on + strand at 313.634 kb on + strand at 313.635 kb on - strand at 313.686 kb on + strand at 313.686 kb on + strand at 313.686 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 310,669 + GFF295 0.34 +0.2 310,669 + GFF295 0.34 -1.8 310,717 + GFF295 0.41 +0.5 310,717 + GFF295 0.41 +0.5 310,717 + GFF295 0.41 -0.8 310,876 - GFF295 0.64 -1.1 310,876 - GFF295 0.64 -0.8 310,876 - GFF295 0.64 -1.7 311,041 + GFF295 0.88 -2.0 311,042 - GFF295 0.89 +0.2 311,434 + -0.0 311,635 + -1.1 311,762 + -0.1 311,762 + +0.1 311,763 - +0.7 311,763 - +0.1 311,962 + GFF296 0.27 +0.0 311,962 + GFF296 0.27 +0.0 311,962 + GFF296 0.27 +0.3 311,962 + GFF296 0.27 +0.8 311,963 - GFF296 0.27 -1.0 312,097 + GFF296 0.39 -0.3 312,105 + GFF296 0.40 -0.7 312,107 + GFF296 0.40 +0.2 312,107 + GFF296 0.40 -0.2 312,107 + GFF296 0.40 -0.1 312,107 + GFF296 0.40 -0.5 312,107 + GFF296 0.40 +0.2 312,164 + GFF296 0.45 +0.0 312,164 + GFF296 0.45 -0.5 312,164 + GFF296 0.45 +0.3 312,164 + GFF296 0.45 +1.2 312,165 - GFF296 0.45 +0.0 312,165 - GFF296 0.45 -1.0 312,165 - GFF296 0.45 +0.4 312,217 - GFF296 0.50 -1.8 312,398 + GFF296 0.66 +1.0 312,398 + GFF296 0.66 +0.1 312,398 + GFF296 0.66 +0.2 312,629 + GFF296 0.86 +0.5 312,629 + GFF296 0.86 +0.2 312,630 - GFF296 0.86 -0.2 312,630 - GFF296 0.86 +0.1 312,704 + +0.7 312,704 + -1.3 312,705 - +0.3 312,705 - -0.2 312,705 - -0.3 312,705 - +1.7 312,705 - -0.4 312,705 - +1.1 312,705 - -0.3 312,740 + -0.5 312,741 - +0.5 312,741 - -1.3 312,741 - -0.8 312,746 + -0.1 312,746 + +0.1 312,746 + -0.2 312,747 - +0.0 312,747 - +2.5 312,747 - -1.7 312,747 - +0.5 312,747 - -0.5 312,747 - +0.6 312,747 - -0.6 312,747 - +1.7 312,784 - -0.3 312,936 + GFF297 0.62 -0.8 312,960 - GFF297 0.71 -0.6 312,961 + GFF297 0.72 +0.3 312,961 + GFF297 0.72 -0.3 312,961 + GFF297 0.72 -0.1 313,194 - GFF298 0.81 -0.4 313,194 - GFF298 0.81 +0.8 313,289 + GFF299 0.11 +1.2 313,379 + GFF299 0.32 -0.9 313,380 - GFF299 0.32 +0.6 313,387 + GFF299 0.34 -0.2 313,387 + GFF299 0.34 -0.1 313,387 + GFF299 0.34 +1.2 313,387 + GFF299 0.34 +0.1 313,387 + GFF299 0.34 +0.5 313,387 + GFF299 0.34 -0.6 313,387 + GFF299 0.34 -0.2 313,387 + GFF299 0.34 -1.4 313,388 - GFF299 0.34 -0.3 313,388 - GFF299 0.34 +1.1 313,388 - GFF299 0.34 +0.2 313,436 + GFF299 0.45 -0.3 313,491 + GFF299 0.58 -0.2 313,493 + GFF299 0.59 -0.1 313,493 + GFF299 0.59 -0.8 313,493 + GFF299 0.59 -0.0 313,493 + GFF299 0.59 -0.9 313,494 - GFF299 0.59 +0.3 313,494 - GFF299 0.59 -1.5 313,494 - GFF299 0.59 +0.2 313,494 - GFF299 0.59 +0.0 313,634 + +0.3 313,634 + -0.3 313,635 - +1.2 313,686 + +0.5 313,686 + -0.6 313,686 + -0.6
Or see this region's nucleotide sequence