Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2242 and GFF2243 are separated by 43 nucleotides GFF2243 and GFF2244 are separated by 129 nucleotides GFF2244 and GFF2245 are separated by 49 nucleotides
GFF2242 - Carbamoyltransferase HypF, at 946,029 to 948,338
GFF2242
GFF2243 - Ribosomal RNA small subunit methyltransferase I, at 948,382 to 949,353
GFF2243
GFF2244 - hypothetical protein, at 949,483 to 949,746
GFF2244
GFF2245 - Glutathione synthetase, at 949,796 to 950,761
GFF2245
Position (kb) 948
949
950 Strain fitness (log2 ratio) -2
-1
0
1
2 at 947.994 kb on - strand, within GFF2242 at 948.976 kb on + strand, within GFF2243 at 949.117 kb on + strand, within GFF2243 at 949.294 kb on + strand at 949.294 kb on + strand at 949.295 kb on - strand at 949.295 kb on - strand at 949.633 kb on + strand, within GFF2244 at 949.633 kb on + strand, within GFF2244 at 949.634 kb on - strand, within GFF2244 at 949.775 kb on - strand at 949.776 kb on + strand at 949.776 kb on + strand at 949.777 kb on - strand at 949.777 kb on - strand at 949.777 kb on - strand at 949.777 kb on - strand at 949.780 kb on + strand at 949.780 kb on + strand at 949.780 kb on + strand at 949.781 kb on - strand at 949.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 947,994 - GFF2242 0.85 -0.4 948,976 + GFF2243 0.61 +0.6 949,117 + GFF2243 0.76 -2.1 949,294 + +0.6 949,294 + +0.3 949,295 - -1.0 949,295 - -0.4 949,633 + GFF2244 0.57 +0.4 949,633 + GFF2244 0.57 +2.0 949,634 - GFF2244 0.57 -0.9 949,775 - +0.9 949,776 + -0.9 949,776 + -0.2 949,777 - -0.2 949,777 - -0.8 949,777 - +0.4 949,777 - -0.8 949,780 + -0.4 949,780 + +0.0 949,780 + +0.4 949,781 - +0.7 949,781 - +0.3
Or see this region's nucleotide sequence