Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1987 and GFF1988 overlap by 4 nucleotides GFF1988 and GFF1989 are separated by 9 nucleotides GFF1989 and GFF1990 are separated by 12 nucleotides GFF1990 and GFF1991 overlap by 4 nucleotides
GFF1987 - hypothetical protein, at 651,888 to 653,480
GFF1987
GFF1988 - hypothetical protein, at 653,477 to 653,779
GFF1988
GFF1989 - Adaptive-response sensory-kinase SasA, at 653,789 to 656,563
GFF1989
GFF1990 - Sensor histidine kinase RcsC, at 656,576 to 657,019
GFF1990
GFF1991 - 3'-5' exonuclease DinG, at 657,016 to 659,190
GFF1991
Position (kb) 653
654
655
656
657 Strain fitness (log2 ratio) -2
-1
0
1 at 652.873 kb on + strand, within GFF1987 at 653.037 kb on + strand, within GFF1987 at 653.038 kb on - strand, within GFF1987 at 653.461 kb on - strand at 653.476 kb on + strand at 653.476 kb on + strand at 653.477 kb on - strand at 653.477 kb on - strand at 653.477 kb on - strand at 653.837 kb on + strand at 653.837 kb on + strand at 653.837 kb on + strand at 653.838 kb on - strand at 653.921 kb on + strand at 653.921 kb on + strand at 653.921 kb on + strand at 653.921 kb on + strand at 653.922 kb on - strand at 653.922 kb on - strand at 653.942 kb on + strand at 653.942 kb on + strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.960 kb on + strand at 653.961 kb on - strand at 653.961 kb on - strand at 654.015 kb on - strand at 654.015 kb on - strand at 654.086 kb on + strand, within GFF1989 at 654.087 kb on - strand, within GFF1989 at 654.189 kb on - strand, within GFF1989 at 654.189 kb on - strand, within GFF1989 at 654.189 kb on - strand, within GFF1989 at 654.189 kb on - strand, within GFF1989 at 654.440 kb on + strand, within GFF1989 at 654.671 kb on + strand, within GFF1989 at 654.809 kb on + strand, within GFF1989 at 654.810 kb on - strand, within GFF1989 at 654.810 kb on - strand, within GFF1989 at 655.002 kb on - strand, within GFF1989 at 655.031 kb on + strand, within GFF1989 at 655.031 kb on + strand, within GFF1989 at 655.031 kb on + strand, within GFF1989 at 655.031 kb on + strand, within GFF1989 at 655.031 kb on + strand, within GFF1989 at 655.032 kb on - strand, within GFF1989 at 655.032 kb on - strand, within GFF1989 at 655.106 kb on + strand, within GFF1989 at 655.107 kb on - strand, within GFF1989 at 655.107 kb on - strand, within GFF1989 at 655.107 kb on - strand, within GFF1989 at 655.107 kb on - strand, within GFF1989 at 655.107 kb on - strand, within GFF1989 at 655.173 kb on - strand, within GFF1989 at 655.173 kb on - strand, within GFF1989 at 655.173 kb on - strand, within GFF1989 at 655.343 kb on + strand, within GFF1989 at 655.343 kb on + strand, within GFF1989 at 655.343 kb on + strand, within GFF1989 at 655.343 kb on + strand, within GFF1989 at 655.343 kb on + strand, within GFF1989 at 655.344 kb on - strand, within GFF1989 at 655.344 kb on - strand, within GFF1989 at 655.736 kb on + strand, within GFF1989 at 655.736 kb on + strand, within GFF1989 at 655.736 kb on + strand, within GFF1989 at 655.737 kb on - strand, within GFF1989 at 656.930 kb on + strand, within GFF1990 at 656.930 kb on + strand, within GFF1990 at 656.930 kb on + strand, within GFF1990 at 656.930 kb on + strand, within GFF1990 at 656.930 kb on + strand, within GFF1990 at 656.931 kb on - strand, within GFF1990 at 657.244 kb on + strand, within GFF1991 at 657.244 kb on + strand, within GFF1991 at 657.244 kb on + strand, within GFF1991 at 657.244 kb on + strand, within GFF1991 at 657.244 kb on + strand, within GFF1991 at 657.245 kb on - strand, within GFF1991 at 657.245 kb on - strand, within GFF1991
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 652,873 + GFF1987 0.62 +0.1 653,037 + GFF1987 0.72 -0.7 653,038 - GFF1987 0.72 -0.9 653,461 - -0.3 653,476 + -0.2 653,476 + +0.6 653,477 - +0.5 653,477 - -0.2 653,477 - +0.3 653,837 + +0.0 653,837 + -0.2 653,837 + -1.4 653,838 - +0.3 653,921 + +0.1 653,921 + -0.3 653,921 + -1.1 653,921 + -0.2 653,922 - +1.0 653,922 - -0.0 653,942 + +0.4 653,942 + +0.1 653,943 - +0.2 653,943 - -0.8 653,943 - +0.6 653,960 + -0.5 653,961 - -1.5 653,961 - +0.2 654,015 - +0.2 654,015 - +0.6 654,086 + GFF1989 0.11 +0.9 654,087 - GFF1989 0.11 -1.1 654,189 - GFF1989 0.14 +0.5 654,189 - GFF1989 0.14 -0.0 654,189 - GFF1989 0.14 +0.2 654,189 - GFF1989 0.14 -0.4 654,440 + GFF1989 0.23 -0.9 654,671 + GFF1989 0.32 -1.0 654,809 + GFF1989 0.37 +0.6 654,810 - GFF1989 0.37 +0.8 654,810 - GFF1989 0.37 +1.0 655,002 - GFF1989 0.44 +0.4 655,031 + GFF1989 0.45 -0.5 655,031 + GFF1989 0.45 +0.6 655,031 + GFF1989 0.45 +0.4 655,031 + GFF1989 0.45 -0.8 655,031 + GFF1989 0.45 -0.2 655,032 - GFF1989 0.45 +0.0 655,032 - GFF1989 0.45 -0.2 655,106 + GFF1989 0.47 -0.7 655,107 - GFF1989 0.47 -0.2 655,107 - GFF1989 0.47 +0.4 655,107 - GFF1989 0.47 -1.2 655,107 - GFF1989 0.47 +0.6 655,107 - GFF1989 0.47 +0.1 655,173 - GFF1989 0.50 +0.3 655,173 - GFF1989 0.50 +0.5 655,173 - GFF1989 0.50 -0.8 655,343 + GFF1989 0.56 -0.5 655,343 + GFF1989 0.56 +0.5 655,343 + GFF1989 0.56 -0.0 655,343 + GFF1989 0.56 -0.4 655,343 + GFF1989 0.56 +0.4 655,344 - GFF1989 0.56 +0.7 655,344 - GFF1989 0.56 -1.1 655,736 + GFF1989 0.70 -0.0 655,736 + GFF1989 0.70 -0.4 655,736 + GFF1989 0.70 +0.2 655,737 - GFF1989 0.70 -0.2 656,930 + GFF1990 0.80 -0.6 656,930 + GFF1990 0.80 -0.7 656,930 + GFF1990 0.80 +0.6 656,930 + GFF1990 0.80 +1.3 656,930 + GFF1990 0.80 +0.3 656,931 - GFF1990 0.80 +0.9 657,244 + GFF1991 0.10 -2.0 657,244 + GFF1991 0.10 -1.2 657,244 + GFF1991 0.10 +0.0 657,244 + GFF1991 0.10 -0.8 657,244 + GFF1991 0.10 -0.2 657,245 - GFF1991 0.11 +0.0 657,245 - GFF1991 0.11 -1.0
Or see this region's nucleotide sequence