Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1957 and GFF1958 are separated by 318 nucleotides GFF1958 and GFF1959 are separated by 225 nucleotides GFF1959 and GFF1960 are separated by 201 nucleotides
GFF1957 - 2-hydroxy-3-oxopropionate reductase, at 613,070 to 613,957
GFF1957
GFF1958 - Pyruvate kinase, at 614,276 to 615,793
GFF1958
GFF1959 - Inorganic triphosphatase, at 616,019 to 616,483
GFF1959
GFF1960 - hypothetical protein, at 616,685 to 617,569
GFF1960
Position (kb) 614
615
616 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 613.409 kb on + strand, within GFF1957 at 613.409 kb on + strand, within GFF1957 at 613.410 kb on - strand, within GFF1957 at 613.410 kb on - strand, within GFF1957 at 614.196 kb on - strand at 614.202 kb on - strand at 614.202 kb on - strand at 615.102 kb on - strand, within GFF1958 at 615.200 kb on + strand, within GFF1958 at 615.200 kb on + strand, within GFF1958 at 615.201 kb on - strand, within GFF1958 at 615.201 kb on - strand, within GFF1958 at 615.386 kb on + strand, within GFF1958 at 615.387 kb on - strand, within GFF1958 at 615.387 kb on - strand, within GFF1958 at 615.387 kb on - strand, within GFF1958 at 615.537 kb on - strand, within GFF1958 at 615.877 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 616.104 kb on - strand, within GFF1959 at 616.104 kb on - strand, within GFF1959 at 616.104 kb on - strand, within GFF1959 at 616.104 kb on - strand, within GFF1959 at 616.283 kb on + strand, within GFF1959 at 616.283 kb on + strand, within GFF1959 at 616.284 kb on - strand, within GFF1959 at 616.284 kb on - strand, within GFF1959 at 616.436 kb on + strand, within GFF1959 at 616.481 kb on + strand at 616.482 kb on - strand at 616.482 kb on - strand at 616.495 kb on - strand at 616.636 kb on - strand at 616.636 kb on - strand at 616.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 613,409 + GFF1957 0.38 +0.1 613,409 + GFF1957 0.38 +0.8 613,410 - GFF1957 0.38 -0.2 613,410 - GFF1957 0.38 -0.5 614,196 - +0.1 614,202 - -0.1 614,202 - -1.2 615,102 - GFF1958 0.54 -0.9 615,200 + GFF1958 0.61 -0.4 615,200 + GFF1958 0.61 -0.3 615,201 - GFF1958 0.61 -0.8 615,201 - GFF1958 0.61 -0.2 615,386 + GFF1958 0.73 -1.4 615,387 - GFF1958 0.73 -0.4 615,387 - GFF1958 0.73 -0.7 615,387 - GFF1958 0.73 -0.6 615,537 - GFF1958 0.83 -0.4 615,877 + +1.0 615,984 + +0.6 615,984 + -0.2 615,985 - -0.4 615,985 - +0.0 615,985 - -1.3 615,985 - -0.5 615,985 - -2.8 615,985 - -0.6 616,104 - GFF1959 0.18 -0.4 616,104 - GFF1959 0.18 +0.2 616,104 - GFF1959 0.18 +0.8 616,104 - GFF1959 0.18 -0.6 616,283 + GFF1959 0.57 +0.3 616,283 + GFF1959 0.57 -0.3 616,284 - GFF1959 0.57 +0.4 616,284 - GFF1959 0.57 -0.5 616,436 + GFF1959 0.90 -0.7 616,481 + -0.2 616,482 - +0.8 616,482 - -0.3 616,495 - -0.5 616,636 - -1.7 616,636 - -0.1 616,636 - -0.4
Or see this region's nucleotide sequence