Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1848 and GFF1849 are separated by 16 nucleotides GFF1849 and GFF1850 are separated by 7 nucleotides GFF1850 and GFF1851 are separated by 72 nucleotides
GFF1848 - L-aspartate oxidase, at 508,225 to 509,751
GFF1848
GFF1849 - putative nicotinate-nucleotide pyrophosphorylase [carboxylating], at 509,768 to 510,622
GFF1849
GFF1850 - hypothetical protein, at 510,630 to 511,418
GFF1850
GFF1851 - hypothetical protein, at 511,491 to 512,501
GFF1851
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 509.756 kb on + strand at 509.756 kb on + strand at 510.215 kb on + strand, within GFF1849 at 510.470 kb on - strand, within GFF1849 at 510.470 kb on - strand, within GFF1849 at 510.470 kb on - strand, within GFF1849 at 511.147 kb on + strand, within GFF1850 at 511.147 kb on + strand, within GFF1850 at 511.148 kb on - strand, within GFF1850 at 511.264 kb on + strand, within GFF1850 at 511.312 kb on + strand, within GFF1850 at 511.313 kb on - strand, within GFF1850 at 511.313 kb on - strand, within GFF1850 at 511.313 kb on - strand, within GFF1850 at 511.313 kb on - strand, within GFF1850 at 511.313 kb on - strand, within GFF1850 at 511.396 kb on + strand at 511.422 kb on + strand at 511.422 kb on + strand at 511.423 kb on - strand at 511.423 kb on - strand at 511.423 kb on - strand at 511.920 kb on + strand, within GFF1851 at 511.921 kb on - strand, within GFF1851 at 511.973 kb on + strand, within GFF1851 at 512.044 kb on - strand, within GFF1851 at 512.044 kb on - strand, within GFF1851 at 512.373 kb on + strand, within GFF1851 at 512.374 kb on - strand, within GFF1851 at 512.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 509,756 + -0.1 509,756 + +0.1 510,215 + GFF1849 0.52 -1.1 510,470 - GFF1849 0.82 -0.1 510,470 - GFF1849 0.82 -1.8 510,470 - GFF1849 0.82 -2.1 511,147 + GFF1850 0.66 +1.0 511,147 + GFF1850 0.66 +0.2 511,148 - GFF1850 0.66 +0.0 511,264 + GFF1850 0.80 -0.5 511,312 + GFF1850 0.86 +0.8 511,313 - GFF1850 0.87 -0.0 511,313 - GFF1850 0.87 -1.1 511,313 - GFF1850 0.87 -0.1 511,313 - GFF1850 0.87 +0.3 511,313 - GFF1850 0.87 +0.7 511,396 + +1.0 511,422 + +0.4 511,422 + -0.1 511,423 - +0.5 511,423 - -0.2 511,423 - +0.5 511,920 + GFF1851 0.42 +0.3 511,921 - GFF1851 0.43 +0.8 511,973 + GFF1851 0.48 +0.0 512,044 - GFF1851 0.55 +0.5 512,044 - GFF1851 0.55 +0.0 512,373 + GFF1851 0.87 +1.9 512,374 - GFF1851 0.87 +1.7 512,413 - +0.9
Or see this region's nucleotide sequence