Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1790 and GFF1791 are separated by 80 nucleotides GFF1791 and GFF1792 are separated by 113 nucleotides GFF1792 and GFF1793 are separated by 215 nucleotides GFF1793 and GFF1794 are separated by 24 nucleotides
GFF1790 - putative 3-hydroxyisobutyrate dehydrogenase, at 439,644 to 440,543
GFF1790
GFF1791 - Inner membrane protein YhaH, at 440,624 to 441,058
GFF1791
GFF1792 - Putative aminoacrylate hydrolase RutD, at 441,172 to 442,137
GFF1792
GFF1793 - Aklanonic acid methyltransferase DauC, at 442,353 to 442,997
GFF1793
GFF1794 - tRNA-specific 2-thiouridylase MnmA, at 443,022 to 444,203
GFF1794
Position (kb)
441
442
443 Strain fitness (log2 ratio)
-1
0
1 at 440.298 kb on + strand, within GFF1790 at 440.298 kb on + strand, within GFF1790 at 440.298 kb on + strand, within GFF1790 at 440.298 kb on + strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.299 kb on - strand, within GFF1790 at 440.346 kb on + strand, within GFF1790 at 440.346 kb on + strand, within GFF1790 at 440.347 kb on - strand, within GFF1790 at 440.378 kb on + strand, within GFF1790 at 440.458 kb on - strand at 440.458 kb on - strand at 440.664 kb on + strand at 440.862 kb on + strand, within GFF1791 at 441.018 kb on + strand at 441.018 kb on + strand at 441.019 kb on - strand at 441.104 kb on + strand at 441.104 kb on + strand at 441.105 kb on - strand at 441.320 kb on - strand, within GFF1792 at 441.878 kb on + strand, within GFF1792 at 442.157 kb on - strand at 442.157 kb on - strand at 442.281 kb on - strand at 442.985 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 440,298 + GFF1790 0.73 +0.4 440,298 + GFF1790 0.73 -0.3 440,298 + GFF1790 0.73 +0.3 440,298 + GFF1790 0.73 +0.0 440,299 - GFF1790 0.73 +1.3 440,299 - GFF1790 0.73 +0.5 440,299 - GFF1790 0.73 -0.2 440,299 - GFF1790 0.73 +0.3 440,299 - GFF1790 0.73 -0.2 440,299 - GFF1790 0.73 -0.5 440,299 - GFF1790 0.73 -0.5 440,346 + GFF1790 0.78 +0.7 440,346 + GFF1790 0.78 -0.9 440,347 - GFF1790 0.78 -0.4 440,378 + GFF1790 0.82 -0.1 440,458 - -0.6 440,458 - +0.4 440,664 + +1.2 440,862 + GFF1791 0.55 +0.2 441,018 + -0.1 441,018 + -0.2 441,019 - +0.5 441,104 + -1.1 441,104 + -0.7 441,105 - +1.1 441,320 - GFF1792 0.15 -0.8 441,878 + GFF1792 0.73 -0.6 442,157 - -0.5 442,157 - +0.6 442,281 - +1.4 442,985 - +0.1
Or see this region's nucleotide sequence