Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1580 and GFF1581 overlap by 20 nucleotides GFF1581 and GFF1582 overlap by 4 nucleotides GFF1582 and GFF1583 overlap by 4 nucleotides GFF1583 and GFF1584 are separated by 85 nucleotides
GFF1580 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 226,855 to 227,571
GFF1580
GFF1581 - Lipopolysaccharide export system ATP-binding protein LptB, at 227,552 to 228,289
GFF1581
GFF1582 - hypothetical protein, at 228,286 to 229,332
GFF1582
GFF1583 - High-affinity branched-chain amino acid transport system permease protein LivH, at 229,329 to 230,213
GFF1583
GFF1584 - hypothetical protein, at 230,299 to 231,597
GFF1584
Position (kb) 228
229
230 Strain fitness (log2 ratio) -2
-1
0
1
2 at 227.528 kb on + strand at 227.529 kb on - strand at 227.529 kb on - strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.571 kb on + strand at 227.572 kb on - strand at 227.572 kb on - strand at 227.586 kb on + strand at 228.370 kb on - strand at 228.428 kb on + strand, within GFF1582 at 228.428 kb on + strand, within GFF1582 at 228.429 kb on - strand, within GFF1582 at 228.569 kb on + strand, within GFF1582 at 228.569 kb on + strand, within GFF1582 at 228.569 kb on + strand, within GFF1582 at 228.587 kb on + strand, within GFF1582 at 228.587 kb on + strand, within GFF1582 at 228.588 kb on - strand, within GFF1582 at 228.588 kb on - strand, within GFF1582 at 228.588 kb on - strand, within GFF1582 at 228.644 kb on + strand, within GFF1582 at 228.660 kb on + strand, within GFF1582 at 228.711 kb on - strand, within GFF1582 at 228.711 kb on - strand, within GFF1582 at 228.779 kb on + strand, within GFF1582 at 228.780 kb on - strand, within GFF1582 at 228.782 kb on + strand, within GFF1582 at 228.782 kb on + strand, within GFF1582 at 228.782 kb on + strand, within GFF1582 at 228.786 kb on - strand, within GFF1582 at 228.865 kb on + strand, within GFF1582 at 228.866 kb on - strand, within GFF1582 at 228.891 kb on + strand, within GFF1582 at 228.947 kb on + strand, within GFF1582 at 228.975 kb on - strand, within GFF1582 at 229.046 kb on + strand, within GFF1582 at 229.046 kb on + strand, within GFF1582 at 229.046 kb on + strand, within GFF1582 at 229.047 kb on - strand, within GFF1582 at 229.047 kb on - strand, within GFF1582 at 229.047 kb on - strand, within GFF1582 at 229.047 kb on - strand, within GFF1582 at 229.073 kb on + strand, within GFF1582 at 229.073 kb on + strand, within GFF1582 at 229.074 kb on - strand, within GFF1582 at 229.074 kb on - strand, within GFF1582 at 229.074 kb on - strand, within GFF1582 at 229.124 kb on + strand, within GFF1582 at 229.125 kb on - strand, within GFF1582 at 229.125 kb on - strand, within GFF1582 at 229.125 kb on - strand, within GFF1582 at 229.158 kb on - strand, within GFF1582 at 229.625 kb on - strand, within GFF1583 at 229.625 kb on - strand, within GFF1583 at 229.625 kb on - strand, within GFF1583 at 229.813 kb on + strand, within GFF1583 at 229.813 kb on + strand, within GFF1583 at 229.813 kb on + strand, within GFF1583 at 229.813 kb on + strand, within GFF1583 at 229.816 kb on + strand, within GFF1583 at 229.978 kb on + strand, within GFF1583 at 230.065 kb on + strand, within GFF1583 at 230.065 kb on + strand, within GFF1583 at 230.066 kb on - strand, within GFF1583 at 230.066 kb on - strand, within GFF1583 at 230.156 kb on - strand at 230.156 kb on - strand at 230.156 kb on - strand at 230.156 kb on - strand at 230.156 kb on - strand at 230.156 kb on - strand at 230.156 kb on - strand at 230.239 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 227,528 + -0.2 227,529 - +0.5 227,529 - +0.7 227,571 + -0.3 227,571 + -0.7 227,571 + +0.2 227,571 + +0.1 227,571 + +0.3 227,571 + -0.6 227,571 + +1.6 227,571 + +0.0 227,571 + -0.7 227,572 - +0.6 227,572 - -0.6 227,586 + +0.0 228,370 - -0.3 228,428 + GFF1582 0.14 -0.6 228,428 + GFF1582 0.14 -0.7 228,429 - GFF1582 0.14 -0.0 228,569 + GFF1582 0.27 +0.6 228,569 + GFF1582 0.27 +1.8 228,569 + GFF1582 0.27 +0.3 228,587 + GFF1582 0.29 -0.5 228,587 + GFF1582 0.29 -0.1 228,588 - GFF1582 0.29 +0.7 228,588 - GFF1582 0.29 +1.0 228,588 - GFF1582 0.29 -0.3 228,644 + GFF1582 0.34 +0.3 228,660 + GFF1582 0.36 +0.1 228,711 - GFF1582 0.41 -0.2 228,711 - GFF1582 0.41 +0.8 228,779 + GFF1582 0.47 -0.6 228,780 - GFF1582 0.47 +0.1 228,782 + GFF1582 0.47 -0.0 228,782 + GFF1582 0.47 -0.3 228,782 + GFF1582 0.47 +1.1 228,786 - GFF1582 0.48 +1.4 228,865 + GFF1582 0.55 +0.8 228,866 - GFF1582 0.55 +0.1 228,891 + GFF1582 0.58 +0.0 228,947 + GFF1582 0.63 -0.3 228,975 - GFF1582 0.66 +1.6 229,046 + GFF1582 0.73 -0.3 229,046 + GFF1582 0.73 -0.6 229,046 + GFF1582 0.73 +0.2 229,047 - GFF1582 0.73 +0.4 229,047 - GFF1582 0.73 -0.5 229,047 - GFF1582 0.73 -0.5 229,047 - GFF1582 0.73 -0.2 229,073 + GFF1582 0.75 -1.9 229,073 + GFF1582 0.75 -0.5 229,074 - GFF1582 0.75 +0.8 229,074 - GFF1582 0.75 +0.5 229,074 - GFF1582 0.75 -1.0 229,124 + GFF1582 0.80 -0.5 229,125 - GFF1582 0.80 +0.1 229,125 - GFF1582 0.80 -0.1 229,125 - GFF1582 0.80 +1.1 229,158 - GFF1582 0.83 +0.6 229,625 - GFF1583 0.33 +0.0 229,625 - GFF1583 0.33 +0.3 229,625 - GFF1583 0.33 +0.3 229,813 + GFF1583 0.55 -0.1 229,813 + GFF1583 0.55 -0.6 229,813 + GFF1583 0.55 -1.0 229,813 + GFF1583 0.55 +1.3 229,816 + GFF1583 0.55 +0.9 229,978 + GFF1583 0.73 +0.1 230,065 + GFF1583 0.83 +0.3 230,065 + GFF1583 0.83 -0.1 230,066 - GFF1583 0.83 +0.3 230,066 - GFF1583 0.83 +0.3 230,156 - -1.2 230,156 - +0.7 230,156 - -0.5 230,156 - +0.1 230,156 - +0.9 230,156 - +0.7 230,156 - +1.2 230,239 + +0.1
Or see this region's nucleotide sequence