Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1433 and GFF1434 overlap by 4 nucleotides GFF1434 and GFF1435 are separated by 9 nucleotides GFF1435 and GFF1436 are separated by 86 nucleotides GFF1436 and GFF1437 are separated by 45 nucleotides
GFF1433 - 3-oxoadipate CoA-transferase subunit A, at 57,062 to 57,784
GFF1433
GFF1434 - 3-oxoadipate CoA-transferase subunit B, at 57,781 to 58,464
GFF1434
GFF1435 - Beta-ketoadipyl-CoA thiolase, at 58,474 to 59,676
GFF1435
GFF1436 - NAD(P)H-dependent FMN reductase, at 59,763 to 60,326
GFF1436
GFF1437 - hypothetical protein, at 60,372 to 60,659
GFF1437
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1 at 57.503 kb on + strand, within GFF1433 at 57.503 kb on + strand, within GFF1433 at 57.504 kb on - strand, within GFF1433 at 57.504 kb on - strand, within GFF1433 at 57.581 kb on + strand, within GFF1433 at 57.581 kb on + strand, within GFF1433 at 57.776 kb on + strand at 57.776 kb on + strand at 57.776 kb on + strand at 57.777 kb on - strand at 57.777 kb on - strand at 57.798 kb on + strand at 57.859 kb on + strand, within GFF1434 at 57.859 kb on + strand, within GFF1434 at 57.859 kb on + strand, within GFF1434 at 57.859 kb on + strand, within GFF1434 at 57.860 kb on - strand, within GFF1434 at 57.928 kb on + strand, within GFF1434 at 57.928 kb on + strand, within GFF1434 at 57.929 kb on - strand, within GFF1434 at 57.929 kb on - strand, within GFF1434 at 58.111 kb on + strand, within GFF1434 at 58.111 kb on + strand, within GFF1434 at 58.111 kb on + strand, within GFF1434 at 58.111 kb on + strand, within GFF1434 at 58.312 kb on + strand, within GFF1434 at 58.312 kb on + strand, within GFF1434 at 58.314 kb on + strand, within GFF1434 at 58.314 kb on + strand, within GFF1434 at 58.315 kb on - strand, within GFF1434 at 58.315 kb on - strand, within GFF1434 at 58.462 kb on + strand at 58.462 kb on + strand at 58.463 kb on - strand at 58.498 kb on + strand at 58.498 kb on + strand at 58.498 kb on + strand at 58.499 kb on - strand at 58.499 kb on - strand at 59.291 kb on - strand, within GFF1435 at 59.695 kb on - strand at 59.942 kb on - strand, within GFF1436 at 59.942 kb on - strand, within GFF1436 at 60.070 kb on + strand, within GFF1436 at 60.070 kb on + strand, within GFF1436 at 60.070 kb on + strand, within GFF1436 at 60.070 kb on + strand, within GFF1436 at 60.071 kb on - strand, within GFF1436 at 60.071 kb on - strand, within GFF1436 at 60.071 kb on - strand, within GFF1436 at 60.071 kb on - strand, within GFF1436 at 60.076 kb on + strand, within GFF1436 at 60.148 kb on + strand, within GFF1436 at 60.175 kb on + strand, within GFF1436 at 60.176 kb on - strand, within GFF1436 at 60.176 kb on - strand, within GFF1436 at 60.262 kb on + strand, within GFF1436 at 60.262 kb on + strand, within GFF1436 at 60.263 kb on - strand, within GFF1436 at 60.263 kb on - strand, within GFF1436 at 60.263 kb on - strand, within GFF1436 at 60.263 kb on - strand, within GFF1436 at 60.263 kb on - strand, within GFF1436 at 60.326 kb on + strand at 60.326 kb on + strand at 60.326 kb on + strand at 60.326 kb on + strand at 60.326 kb on + strand at 60.327 kb on - strand at 60.327 kb on - strand at 60.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 57,503 + GFF1433 0.61 -0.6 57,503 + GFF1433 0.61 +0.1 57,504 - GFF1433 0.61 +0.0 57,504 - GFF1433 0.61 +0.0 57,581 + GFF1433 0.72 +1.4 57,581 + GFF1433 0.72 +0.1 57,776 + +0.5 57,776 + +0.5 57,776 + +0.6 57,777 - -0.1 57,777 - -0.2 57,798 + +0.7 57,859 + GFF1434 0.11 -0.4 57,859 + GFF1434 0.11 +0.1 57,859 + GFF1434 0.11 -1.9 57,859 + GFF1434 0.11 -0.4 57,860 - GFF1434 0.12 -0.1 57,928 + GFF1434 0.21 -0.6 57,928 + GFF1434 0.21 -0.1 57,929 - GFF1434 0.22 +0.2 57,929 - GFF1434 0.22 -0.3 58,111 + GFF1434 0.48 +0.5 58,111 + GFF1434 0.48 +1.0 58,111 + GFF1434 0.48 +1.3 58,111 + GFF1434 0.48 +0.0 58,312 + GFF1434 0.78 -0.2 58,312 + GFF1434 0.78 +0.8 58,314 + GFF1434 0.78 -1.4 58,314 + GFF1434 0.78 -0.4 58,315 - GFF1434 0.78 -0.4 58,315 - GFF1434 0.78 -0.2 58,462 + -0.1 58,462 + -0.0 58,463 - -0.2 58,498 + +0.7 58,498 + -0.6 58,498 + +0.8 58,499 - -0.2 58,499 - +0.6 59,291 - GFF1435 0.68 -0.8 59,695 - +0.6 59,942 - GFF1436 0.32 +0.9 59,942 - GFF1436 0.32 -0.6 60,070 + GFF1436 0.54 +1.5 60,070 + GFF1436 0.54 -0.8 60,070 + GFF1436 0.54 -0.7 60,070 + GFF1436 0.54 +0.1 60,071 - GFF1436 0.55 +1.0 60,071 - GFF1436 0.55 -0.0 60,071 - GFF1436 0.55 +1.1 60,071 - GFF1436 0.55 -0.6 60,076 + GFF1436 0.55 -1.4 60,148 + GFF1436 0.68 -0.5 60,175 + GFF1436 0.73 -0.0 60,176 - GFF1436 0.73 -0.2 60,176 - GFF1436 0.73 -0.6 60,262 + GFF1436 0.88 +0.5 60,262 + GFF1436 0.88 +0.2 60,263 - GFF1436 0.89 -0.4 60,263 - GFF1436 0.89 +0.7 60,263 - GFF1436 0.89 +0.6 60,263 - GFF1436 0.89 +0.4 60,263 - GFF1436 0.89 +0.9 60,326 + +0.4 60,326 + +1.1 60,326 + +0.8 60,326 + +0.9 60,326 + +0.4 60,327 - +0.0 60,327 - +0.0 60,327 - -1.6
Or see this region's nucleotide sequence