Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF138 and GFF139 are separated by 197 nucleotides GFF139 and GFF140 overlap by 4 nucleotides GFF140 and GFF141 are separated by 256 nucleotides GFF141 and GFF142 are separated by 175 nucleotides
GFF138 - 2-hydroxychromene-2-carboxylate isomerase, at 149,307 to 149,951
GFF138
GFF139 - hypothetical protein, at 150,149 to 150,400
GFF139
GFF140 - hypothetical protein, at 150,397 to 150,846
GFF140
GFF141 - hypothetical protein, at 151,103 to 151,555
GFF141
GFF142 - Cytosol aminopeptidase, at 151,731 to 153,227
GFF142
Position (kb) 150
151 Strain fitness (log2 ratio) -2
-1
0
1 at 149.407 kb on + strand, within GFF138 at 149.408 kb on - strand, within GFF138 at 149.462 kb on - strand, within GFF138 at 149.564 kb on - strand, within GFF138 at 149.996 kb on + strand at 149.996 kb on + strand at 149.997 kb on - strand at 149.997 kb on - strand at 149.997 kb on - strand at 149.997 kb on - strand at 149.997 kb on - strand at 150.148 kb on + strand at 150.760 kb on + strand, within GFF140 at 150.760 kb on + strand, within GFF140 at 150.760 kb on + strand, within GFF140 at 151.056 kb on - strand at 151.568 kb on - strand at 151.723 kb on - strand at 151.723 kb on - strand at 151.741 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 149,407 + GFF138 0.16 -0.3 149,408 - GFF138 0.16 +0.2 149,462 - GFF138 0.24 +0.4 149,564 - GFF138 0.40 +0.1 149,996 + -0.4 149,996 + -1.0 149,997 - -1.2 149,997 - +0.5 149,997 - -1.8 149,997 - -0.1 149,997 - +0.8 150,148 + +0.8 150,760 + GFF140 0.81 -0.6 150,760 + GFF140 0.81 -0.2 150,760 + GFF140 0.81 -1.1 151,056 - -0.3 151,568 - +0.6 151,723 - +0.3 151,723 - +0.5 151,741 + +1.0
Or see this region's nucleotide sequence