Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1174 and GFF1175 overlap by 4 nucleotides GFF1175 and GFF1176 are separated by 26 nucleotides GFF1176 and GFF1177 are separated by 36 nucleotides
GFF1174 - Sulfate/thiosulfate import ATP-binding protein CysA, at 1,275,679 to 1,276,386
GFF1174
GFF1175 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 1,276,383 to 1,277,078
GFF1175
GFF1176 - Leu/Ile/Val-binding protein, at 1,277,105 to 1,278,292
GFF1176
GFF1177 - HTH-type transcriptional regulator KipR, at 1,278,329 to 1,279,150
GFF1177
Position (kb)
1277
1278
1279 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1276.419 kb on + strand at 1276.420 kb on - strand at 1276.599 kb on + strand, within GFF1175 at 1276.599 kb on + strand, within GFF1175 at 1276.600 kb on - strand, within GFF1175 at 1276.600 kb on - strand, within GFF1175 at 1276.600 kb on - strand, within GFF1175 at 1277.219 kb on + strand at 1277.219 kb on + strand at 1277.220 kb on - strand at 1277.220 kb on - strand at 1277.220 kb on - strand at 1277.220 kb on - strand at 1277.318 kb on + strand, within GFF1176 at 1277.318 kb on + strand, within GFF1176 at 1277.463 kb on - strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.729 kb on + strand, within GFF1176 at 1277.730 kb on - strand, within GFF1176 at 1277.730 kb on - strand, within GFF1176 at 1277.730 kb on - strand, within GFF1176 at 1277.730 kb on - strand, within GFF1176 at 1277.730 kb on - strand, within GFF1176 at 1277.730 kb on - strand, within GFF1176 at 1277.858 kb on - strand, within GFF1176 at 1277.988 kb on - strand, within GFF1176 at 1278.026 kb on + strand, within GFF1176 at 1278.026 kb on + strand, within GFF1176 at 1278.027 kb on - strand, within GFF1176 at 1278.027 kb on - strand, within GFF1176 at 1278.116 kb on + strand, within GFF1176 at 1278.349 kb on + strand at 1278.473 kb on + strand, within GFF1177 at 1278.607 kb on - strand, within GFF1177 at 1278.717 kb on + strand, within GFF1177 at 1278.717 kb on + strand, within GFF1177 at 1278.793 kb on - strand, within GFF1177 at 1278.849 kb on + strand, within GFF1177 at 1278.850 kb on - strand, within GFF1177 at 1278.850 kb on - strand, within GFF1177 at 1278.852 kb on + strand, within GFF1177 at 1278.852 kb on + strand, within GFF1177 at 1278.853 kb on - strand, within GFF1177 at 1278.981 kb on + strand, within GFF1177 at 1278.981 kb on + strand, within GFF1177 at 1278.981 kb on + strand, within GFF1177 at 1278.981 kb on + strand, within GFF1177 at 1278.981 kb on + strand, within GFF1177 at 1278.982 kb on - strand, within GFF1177 at 1278.982 kb on - strand, within GFF1177 at 1279.035 kb on + strand, within GFF1177 at 1279.035 kb on + strand, within GFF1177 at 1279.036 kb on - strand, within GFF1177 at 1279.036 kb on - strand, within GFF1177 at 1279.036 kb on - strand, within GFF1177 at 1279.212 kb on + strand at 1279.212 kb on + strand at 1279.212 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 1,276,419 + +1.1 1,276,420 - -1.8 1,276,599 + GFF1175 0.31 -0.4 1,276,599 + GFF1175 0.31 +1.2 1,276,600 - GFF1175 0.31 +0.0 1,276,600 - GFF1175 0.31 -0.5 1,276,600 - GFF1175 0.31 -0.2 1,277,219 + +1.0 1,277,219 + +0.2 1,277,220 - -0.5 1,277,220 - +0.3 1,277,220 - +1.0 1,277,220 - -0.5 1,277,318 + GFF1176 0.18 -0.8 1,277,318 + GFF1176 0.18 -0.1 1,277,463 - GFF1176 0.30 -1.0 1,277,729 + GFF1176 0.53 -1.3 1,277,729 + GFF1176 0.53 +0.1 1,277,729 + GFF1176 0.53 +2.0 1,277,729 + GFF1176 0.53 -0.5 1,277,729 + GFF1176 0.53 +0.3 1,277,729 + GFF1176 0.53 -0.5 1,277,729 + GFF1176 0.53 +0.9 1,277,729 + GFF1176 0.53 +0.3 1,277,729 + GFF1176 0.53 -1.1 1,277,730 - GFF1176 0.53 +0.7 1,277,730 - GFF1176 0.53 +0.8 1,277,730 - GFF1176 0.53 -1.0 1,277,730 - GFF1176 0.53 -0.7 1,277,730 - GFF1176 0.53 -0.7 1,277,730 - GFF1176 0.53 +0.5 1,277,858 - GFF1176 0.63 -0.2 1,277,988 - GFF1176 0.74 -0.4 1,278,026 + GFF1176 0.78 +0.3 1,278,026 + GFF1176 0.78 +0.6 1,278,027 - GFF1176 0.78 +0.1 1,278,027 - GFF1176 0.78 +1.2 1,278,116 + GFF1176 0.85 -1.0 1,278,349 + +0.2 1,278,473 + GFF1177 0.18 -0.8 1,278,607 - GFF1177 0.34 -0.1 1,278,717 + GFF1177 0.47 -0.9 1,278,717 + GFF1177 0.47 -1.1 1,278,793 - GFF1177 0.56 +0.8 1,278,849 + GFF1177 0.63 +0.5 1,278,850 - GFF1177 0.63 -1.1 1,278,850 - GFF1177 0.63 -1.2 1,278,852 + GFF1177 0.64 -0.5 1,278,852 + GFF1177 0.64 -1.3 1,278,853 - GFF1177 0.64 +0.1 1,278,981 + GFF1177 0.79 -0.6 1,278,981 + GFF1177 0.79 -2.1 1,278,981 + GFF1177 0.79 -0.5 1,278,981 + GFF1177 0.79 -1.8 1,278,981 + GFF1177 0.79 -0.4 1,278,982 - GFF1177 0.79 -2.2 1,278,982 - GFF1177 0.79 -0.8 1,279,035 + GFF1177 0.86 -0.3 1,279,035 + GFF1177 0.86 +0.7 1,279,036 - GFF1177 0.86 -1.3 1,279,036 - GFF1177 0.86 -1.0 1,279,036 - GFF1177 0.86 -2.3 1,279,212 + +1.1 1,279,212 + -0.0 1,279,212 + -1.0
Or see this region's nucleotide sequence