Strain Fitness in Xanthobacter sp. DMC5 around GFF1150

Experiment: Methanol and casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1148 and GFF1149 are separated by 68 nucleotidesGFF1149 and GFF1150 overlap by 4 nucleotidesGFF1150 and GFF1151 overlap by 11 nucleotides GFF1148 - hypothetical protein, at 1,239,568 to 1,241,880 GFF1148 GFF1149 - CRISPR pre-crRNA endoribonuclease Cas5d, at 1,241,949 to 1,242,626 GFF1149 GFF1150 - hypothetical protein, at 1,242,623 to 1,244,443 GFF1150 GFF1151 - hypothetical protein, at 1,244,433 to 1,245,374 GFF1151 Position (kb) 1242 1243 1244 1245Strain fitness (log2 ratio) -2 -1 0 1at 1241.698 kb on + strandat 1241.698 kb on + strandat 1241.698 kb on + strandat 1241.698 kb on + strandat 1241.699 kb on - strandat 1241.699 kb on - strandat 1241.699 kb on - strandat 1241.813 kb on - strandat 1241.878 kb on + strandat 1241.878 kb on + strandat 1241.878 kb on + strandat 1241.879 kb on - strandat 1241.955 kb on + strandat 1242.030 kb on + strand, within GFF1149at 1242.031 kb on - strand, within GFF1149at 1242.031 kb on - strand, within GFF1149at 1242.288 kb on + strand, within GFF1149at 1242.288 kb on + strand, within GFF1149at 1242.289 kb on - strand, within GFF1149at 1242.289 kb on - strand, within GFF1149at 1242.289 kb on - strand, within GFF1149at 1242.529 kb on - strand, within GFF1149at 1242.653 kb on + strandat 1242.653 kb on + strandat 1242.654 kb on - strandat 1242.696 kb on - strandat 1242.855 kb on + strand, within GFF1150at 1242.855 kb on + strand, within GFF1150at 1242.872 kb on + strand, within GFF1150at 1242.872 kb on + strand, within GFF1150at 1242.872 kb on + strand, within GFF1150at 1242.872 kb on + strand, within GFF1150at 1242.873 kb on - strand, within GFF1150at 1242.873 kb on - strand, within GFF1150at 1242.873 kb on - strand, within GFF1150at 1242.873 kb on - strand, within GFF1150at 1243.089 kb on - strand, within GFF1150at 1243.089 kb on - strand, within GFF1150at 1243.659 kb on - strand, within GFF1150at 1243.709 kb on + strand, within GFF1150at 1243.802 kb on + strand, within GFF1150at 1243.865 kb on + strand, within GFF1150at 1243.866 kb on - strand, within GFF1150at 1243.866 kb on - strand, within GFF1150at 1244.070 kb on - strand, within GFF1150at 1244.096 kb on + strand, within GFF1150at 1244.154 kb on - strand, within GFF1150at 1244.352 kb on - strandat 1244.352 kb on - strandat 1244.352 kb on - strandat 1244.352 kb on - strandat 1244.605 kb on - strand, within GFF1151at 1244.605 kb on - strand, within GFF1151at 1244.646 kb on + strand, within GFF1151at 1244.646 kb on + strand, within GFF1151at 1244.647 kb on - strand, within GFF1151at 1244.647 kb on - strand, within GFF1151at 1244.647 kb on - strand, within GFF1151at 1244.997 kb on + strand, within GFF1151at 1245.373 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol and casaminos (C)
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1,241,698 + +1.3
1,241,698 + -0.1
1,241,698 + +0.6
1,241,698 + +0.4
1,241,699 - -0.8
1,241,699 - +0.3
1,241,699 - +1.0
1,241,813 - -0.2
1,241,878 + +0.0
1,241,878 + +0.0
1,241,878 + -0.3
1,241,879 - +0.0
1,241,955 + -0.3
1,242,030 + GFF1149 0.12 +0.3
1,242,031 - GFF1149 0.12 +0.6
1,242,031 - GFF1149 0.12 -1.5
1,242,288 + GFF1149 0.50 -0.9
1,242,288 + GFF1149 0.50 -0.2
1,242,289 - GFF1149 0.50 +0.5
1,242,289 - GFF1149 0.50 -0.3
1,242,289 - GFF1149 0.50 -0.1
1,242,529 - GFF1149 0.86 -0.5
1,242,653 + +0.1
1,242,653 + -2.4
1,242,654 - +0.2
1,242,696 - -0.4
1,242,855 + GFF1150 0.13 +0.3
1,242,855 + GFF1150 0.13 +1.3
1,242,872 + GFF1150 0.14 -0.2
1,242,872 + GFF1150 0.14 +0.2
1,242,872 + GFF1150 0.14 +0.0
1,242,872 + GFF1150 0.14 +0.7
1,242,873 - GFF1150 0.14 +1.0
1,242,873 - GFF1150 0.14 +0.7
1,242,873 - GFF1150 0.14 +0.2
1,242,873 - GFF1150 0.14 +0.1
1,243,089 - GFF1150 0.26 +1.2
1,243,089 - GFF1150 0.26 -0.8
1,243,659 - GFF1150 0.57 +0.4
1,243,709 + GFF1150 0.60 +0.3
1,243,802 + GFF1150 0.65 +0.7
1,243,865 + GFF1150 0.68 +0.2
1,243,866 - GFF1150 0.68 +0.4
1,243,866 - GFF1150 0.68 -0.1
1,244,070 - GFF1150 0.79 +0.1
1,244,096 + GFF1150 0.81 -0.6
1,244,154 - GFF1150 0.84 -0.4
1,244,352 - +0.4
1,244,352 - +0.5
1,244,352 - +1.0
1,244,352 - +0.3
1,244,605 - GFF1151 0.18 -0.0
1,244,605 - GFF1151 0.18 +1.5
1,244,646 + GFF1151 0.23 +0.2
1,244,646 + GFF1151 0.23 +0.1
1,244,647 - GFF1151 0.23 +0.0
1,244,647 - GFF1151 0.23 +0.1
1,244,647 - GFF1151 0.23 +0.3
1,244,997 + GFF1151 0.60 -0.0
1,245,373 - +1.1

Or see this region's nucleotide sequence