Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1148 and GFF1149 are separated by 68 nucleotides GFF1149 and GFF1150 overlap by 4 nucleotides GFF1150 and GFF1151 overlap by 11 nucleotides
GFF1148 - hypothetical protein, at 1,239,568 to 1,241,880
GFF1148
GFF1149 - CRISPR pre-crRNA endoribonuclease Cas5d, at 1,241,949 to 1,242,626
GFF1149
GFF1150 - hypothetical protein, at 1,242,623 to 1,244,443
GFF1150
GFF1151 - hypothetical protein, at 1,244,433 to 1,245,374
GFF1151
Position (kb)
1242
1243
1244
1245 Strain fitness (log2 ratio)
-2
-1
0
1 at 1241.698 kb on + strand at 1241.698 kb on + strand at 1241.698 kb on + strand at 1241.698 kb on + strand at 1241.699 kb on - strand at 1241.699 kb on - strand at 1241.699 kb on - strand at 1241.813 kb on - strand at 1241.878 kb on + strand at 1241.878 kb on + strand at 1241.878 kb on + strand at 1241.879 kb on - strand at 1241.955 kb on + strand at 1242.030 kb on + strand, within GFF1149 at 1242.031 kb on - strand, within GFF1149 at 1242.031 kb on - strand, within GFF1149 at 1242.288 kb on + strand, within GFF1149 at 1242.288 kb on + strand, within GFF1149 at 1242.289 kb on - strand, within GFF1149 at 1242.289 kb on - strand, within GFF1149 at 1242.289 kb on - strand, within GFF1149 at 1242.529 kb on - strand, within GFF1149 at 1242.653 kb on + strand at 1242.653 kb on + strand at 1242.654 kb on - strand at 1242.696 kb on - strand at 1242.855 kb on + strand, within GFF1150 at 1242.855 kb on + strand, within GFF1150 at 1242.872 kb on + strand, within GFF1150 at 1242.872 kb on + strand, within GFF1150 at 1242.872 kb on + strand, within GFF1150 at 1242.872 kb on + strand, within GFF1150 at 1242.873 kb on - strand, within GFF1150 at 1242.873 kb on - strand, within GFF1150 at 1242.873 kb on - strand, within GFF1150 at 1242.873 kb on - strand, within GFF1150 at 1243.089 kb on - strand, within GFF1150 at 1243.089 kb on - strand, within GFF1150 at 1243.659 kb on - strand, within GFF1150 at 1243.709 kb on + strand, within GFF1150 at 1243.802 kb on + strand, within GFF1150 at 1243.865 kb on + strand, within GFF1150 at 1243.866 kb on - strand, within GFF1150 at 1243.866 kb on - strand, within GFF1150 at 1244.070 kb on - strand, within GFF1150 at 1244.096 kb on + strand, within GFF1150 at 1244.154 kb on - strand, within GFF1150 at 1244.352 kb on - strand at 1244.352 kb on - strand at 1244.352 kb on - strand at 1244.352 kb on - strand at 1244.605 kb on - strand, within GFF1151 at 1244.605 kb on - strand, within GFF1151 at 1244.646 kb on + strand, within GFF1151 at 1244.646 kb on + strand, within GFF1151 at 1244.647 kb on - strand, within GFF1151 at 1244.647 kb on - strand, within GFF1151 at 1244.647 kb on - strand, within GFF1151 at 1244.997 kb on + strand, within GFF1151 at 1245.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 1,241,698 + +1.3 1,241,698 + -0.1 1,241,698 + +0.6 1,241,698 + +0.4 1,241,699 - -0.8 1,241,699 - +0.3 1,241,699 - +1.0 1,241,813 - -0.2 1,241,878 + +0.0 1,241,878 + +0.0 1,241,878 + -0.3 1,241,879 - +0.0 1,241,955 + -0.3 1,242,030 + GFF1149 0.12 +0.3 1,242,031 - GFF1149 0.12 +0.6 1,242,031 - GFF1149 0.12 -1.5 1,242,288 + GFF1149 0.50 -0.9 1,242,288 + GFF1149 0.50 -0.2 1,242,289 - GFF1149 0.50 +0.5 1,242,289 - GFF1149 0.50 -0.3 1,242,289 - GFF1149 0.50 -0.1 1,242,529 - GFF1149 0.86 -0.5 1,242,653 + +0.1 1,242,653 + -2.4 1,242,654 - +0.2 1,242,696 - -0.4 1,242,855 + GFF1150 0.13 +0.3 1,242,855 + GFF1150 0.13 +1.3 1,242,872 + GFF1150 0.14 -0.2 1,242,872 + GFF1150 0.14 +0.2 1,242,872 + GFF1150 0.14 +0.0 1,242,872 + GFF1150 0.14 +0.7 1,242,873 - GFF1150 0.14 +1.0 1,242,873 - GFF1150 0.14 +0.7 1,242,873 - GFF1150 0.14 +0.2 1,242,873 - GFF1150 0.14 +0.1 1,243,089 - GFF1150 0.26 +1.2 1,243,089 - GFF1150 0.26 -0.8 1,243,659 - GFF1150 0.57 +0.4 1,243,709 + GFF1150 0.60 +0.3 1,243,802 + GFF1150 0.65 +0.7 1,243,865 + GFF1150 0.68 +0.2 1,243,866 - GFF1150 0.68 +0.4 1,243,866 - GFF1150 0.68 -0.1 1,244,070 - GFF1150 0.79 +0.1 1,244,096 + GFF1150 0.81 -0.6 1,244,154 - GFF1150 0.84 -0.4 1,244,352 - +0.4 1,244,352 - +0.5 1,244,352 - +1.0 1,244,352 - +0.3 1,244,605 - GFF1151 0.18 -0.0 1,244,605 - GFF1151 0.18 +1.5 1,244,646 + GFF1151 0.23 +0.2 1,244,646 + GFF1151 0.23 +0.1 1,244,647 - GFF1151 0.23 +0.0 1,244,647 - GFF1151 0.23 +0.1 1,244,647 - GFF1151 0.23 +0.3 1,244,997 + GFF1151 0.60 -0.0 1,245,373 - +1.1
Or see this region's nucleotide sequence