Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1026 and GFF1027 are separated by 195 nucleotides GFF1027 and GFF1028 are separated by 239 nucleotides GFF1028 and GFF1029 are separated by 3 nucleotides
GFF1026 - Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase, at 1,107,981 to 1,109,714
GFF1026
GFF1027 - hypothetical protein, at 1,109,910 to 1,110,134
GFF1027
GFF1028 - hypothetical protein, at 1,110,374 to 1,110,610
GFF1028
GFF1029 - Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase, at 1,110,614 to 1,111,546
GFF1029
Position (kb)
1110
1111 Strain fitness (log2 ratio)
-1
0
1 at 1109.429 kb on - strand, within GFF1026 at 1109.448 kb on - strand, within GFF1026 at 1109.483 kb on - strand, within GFF1026 at 1109.753 kb on - strand at 1110.196 kb on - strand at 1110.238 kb on - strand at 1110.238 kb on - strand at 1110.282 kb on + strand at 1110.283 kb on - strand at 1110.283 kb on - strand at 1110.448 kb on - strand, within GFF1028 at 1110.478 kb on - strand, within GFF1028 at 1110.478 kb on - strand, within GFF1028 at 1110.478 kb on - strand, within GFF1028 at 1110.517 kb on - strand, within GFF1028 at 1110.564 kb on + strand, within GFF1028 at 1110.564 kb on + strand, within GFF1028 at 1110.564 kb on + strand, within GFF1028 at 1110.565 kb on - strand, within GFF1028
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 1,109,429 - GFF1026 0.84 +1.7 1,109,448 - GFF1026 0.85 +0.5 1,109,483 - GFF1026 0.87 -0.4 1,109,753 - -0.1 1,110,196 - +0.1 1,110,238 - -0.1 1,110,238 - -0.1 1,110,282 + +0.1 1,110,283 - +0.5 1,110,283 - +1.2 1,110,448 - GFF1028 0.31 +0.7 1,110,478 - GFF1028 0.44 -0.0 1,110,478 - GFF1028 0.44 +0.6 1,110,478 - GFF1028 0.44 -0.9 1,110,517 - GFF1028 0.60 +0.1 1,110,564 + GFF1028 0.80 +0.9 1,110,564 + GFF1028 0.80 +0.1 1,110,564 + GFF1028 0.80 +0.3 1,110,565 - GFF1028 0.81 +0.6
Or see this region's nucleotide sequence