Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF99 and GFF100 are separated by 82 nucleotides GFF100 and GFF101 are separated by 3 nucleotides GFF101 and GFF102 overlap by 4 nucleotides
GFF99 - Tryptophan--tRNA ligase, at 110,380 to 111,414
GFF99
GFF100 - Ubiquinone biosynthesis O-methyltransferase, at 111,497 to 112,240
GFF100
GFF101 - hypothetical protein, at 112,244 to 112,747
GFF101
GFF102 - Deaminated glutathione amidase, at 112,744 to 113,649
GFF102
Position (kb) 112
113 Strain fitness (log2 ratio) -2
-1
0
1 at 111.471 kb on + strand at 111.472 kb on - strand at 112.238 kb on + strand at 112.239 kb on - strand at 112.451 kb on - strand, within GFF101 at 112.677 kb on + strand, within GFF101 at 112.678 kb on - strand, within GFF101 at 112.738 kb on - strand at 112.997 kb on + strand, within GFF102 at 112.998 kb on - strand, within GFF102 at 112.998 kb on - strand, within GFF102 at 113.207 kb on + strand, within GFF102 at 113.225 kb on + strand, within GFF102 at 113.226 kb on - strand, within GFF102 at 113.249 kb on - strand, within GFF102 at 113.249 kb on - strand, within GFF102 at 113.249 kb on - strand, within GFF102 at 113.498 kb on + strand, within GFF102 at 113.723 kb on + strand at 113.723 kb on + strand at 113.723 kb on + strand at 113.723 kb on + strand at 113.724 kb on - strand at 113.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 111,471 + +1.1 111,472 - -1.1 112,238 + -0.4 112,239 - -0.9 112,451 - GFF101 0.41 +0.1 112,677 + GFF101 0.86 -0.0 112,678 - GFF101 0.86 -0.7 112,738 - +0.3 112,997 + GFF102 0.28 -1.3 112,998 - GFF102 0.28 +0.8 112,998 - GFF102 0.28 +0.0 113,207 + GFF102 0.51 -0.1 113,225 + GFF102 0.53 +0.0 113,226 - GFF102 0.53 +0.0 113,249 - GFF102 0.56 -1.6 113,249 - GFF102 0.56 +0.3 113,249 - GFF102 0.56 +0.4 113,498 + GFF102 0.83 +1.2 113,723 + -0.4 113,723 + -1.8 113,723 + -0.0 113,723 + -0.1 113,724 - -0.1 113,724 - +0.4
Or see this region's nucleotide sequence