Experiment: R2A_low_K2HPO4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS09705 and ABZR88_RS09710 are separated by 330 nucleotides ABZR88_RS09710 and ABZR88_RS09715 are separated by 38 nucleotides ABZR88_RS09715 and ABZR88_RS09720 are separated by 51 nucleotides ABZR88_RS09720 and ABZR88_RS09725 are separated by 243 nucleotides
ABZR88_RS09705: ABZR88_RS09705 - helix-turn-helix domain-containing protein, at 2,423,071 to 2,423,706
_RS09705
ABZR88_RS09710: ABZR88_RS09710 - hypothetical protein, at 2,424,037 to 2,424,567
_RS09710
ABZR88_RS09715: ABZR88_RS09715 - site-specific integrase, at 2,424,606 to 2,425,775
_RS09715
ABZR88_RS09720: ABZR88_RS09720 - tRNA-His, at 2,425,827 to 2,425,900
_RS09720
ABZR88_RS09725: ABZR88_RS09725 - DUF6252 family protein, at 2,426,144 to 2,426,605
_RS09725
Position (kb)
2424
2425
2426 Strain fitness (log2 ratio)
-1
0
1
2 at 2423.639 kb on - strand, within ABZR88_RS09705 at 2423.683 kb on - strand at 2423.701 kb on - strand at 2423.704 kb on + strand at 2423.749 kb on - strand at 2423.752 kb on - strand at 2423.791 kb on - strand at 2423.880 kb on + strand at 2423.883 kb on + strand at 2424.011 kb on + strand at 2424.097 kb on + strand, within ABZR88_RS09710 at 2424.170 kb on - strand, within ABZR88_RS09710 at 2424.170 kb on - strand, within ABZR88_RS09710 at 2424.513 kb on + strand, within ABZR88_RS09710 at 2424.527 kb on - strand at 2424.528 kb on + strand at 2424.570 kb on - strand at 2424.702 kb on + strand at 2424.901 kb on - strand, within ABZR88_RS09715 at 2424.921 kb on + strand, within ABZR88_RS09715 at 2424.934 kb on - strand, within ABZR88_RS09715 at 2424.951 kb on + strand, within ABZR88_RS09715 at 2424.977 kb on + strand, within ABZR88_RS09715 at 2424.977 kb on + strand, within ABZR88_RS09715 at 2425.137 kb on + strand, within ABZR88_RS09715 at 2425.153 kb on + strand, within ABZR88_RS09715 at 2425.193 kb on + strand, within ABZR88_RS09715 at 2425.216 kb on + strand, within ABZR88_RS09715 at 2425.277 kb on - strand, within ABZR88_RS09715 at 2425.277 kb on - strand, within ABZR88_RS09715 at 2425.412 kb on + strand, within ABZR88_RS09715 at 2425.456 kb on - strand, within ABZR88_RS09715 at 2425.546 kb on - strand, within ABZR88_RS09715 at 2425.569 kb on - strand, within ABZR88_RS09715 at 2425.682 kb on + strand at 2425.712 kb on - strand at 2425.724 kb on - strand at 2425.749 kb on - strand at 2425.812 kb on + strand at 2425.833 kb on - strand at 2425.989 kb on + strand at 2426.067 kb on - strand at 2426.119 kb on + strand at 2426.137 kb on - strand at 2426.248 kb on + strand, within ABZR88_RS09725 at 2426.249 kb on - strand, within ABZR88_RS09725 at 2426.320 kb on - strand, within ABZR88_RS09725 at 2426.330 kb on - strand, within ABZR88_RS09725 at 2426.330 kb on - strand, within ABZR88_RS09725 at 2426.420 kb on + strand, within ABZR88_RS09725 at 2426.570 kb on + strand at 2426.594 kb on + strand at 2426.651 kb on - strand at 2426.686 kb on - strand at 2426.686 kb on - strand at 2426.729 kb on - strand at 2426.751 kb on - strand at 2426.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_low_K2HPO4 remove 2,423,639 - ABZR88_RS09705 0.89 -0.0 2,423,683 - -0.2 2,423,701 - +0.2 2,423,704 + -0.1 2,423,749 - +0.0 2,423,752 - -0.1 2,423,791 - +1.5 2,423,880 + -0.2 2,423,883 + -0.1 2,424,011 + -0.1 2,424,097 + ABZR88_RS09710 0.11 +0.1 2,424,170 - ABZR88_RS09710 0.25 +0.1 2,424,170 - ABZR88_RS09710 0.25 -0.1 2,424,513 + ABZR88_RS09710 0.90 -0.0 2,424,527 - +0.3 2,424,528 + +0.0 2,424,570 - -0.8 2,424,702 + -0.2 2,424,901 - ABZR88_RS09715 0.25 -0.3 2,424,921 + ABZR88_RS09715 0.27 +0.0 2,424,934 - ABZR88_RS09715 0.28 +0.0 2,424,951 + ABZR88_RS09715 0.29 -0.0 2,424,977 + ABZR88_RS09715 0.32 -0.2 2,424,977 + ABZR88_RS09715 0.32 -0.0 2,425,137 + ABZR88_RS09715 0.45 +0.0 2,425,153 + ABZR88_RS09715 0.47 +0.1 2,425,193 + ABZR88_RS09715 0.50 +0.2 2,425,216 + ABZR88_RS09715 0.52 -0.0 2,425,277 - ABZR88_RS09715 0.57 -0.0 2,425,277 - ABZR88_RS09715 0.57 +1.2 2,425,412 + ABZR88_RS09715 0.69 +0.1 2,425,456 - ABZR88_RS09715 0.73 -0.1 2,425,546 - ABZR88_RS09715 0.80 -0.1 2,425,569 - ABZR88_RS09715 0.82 +0.0 2,425,682 + -0.1 2,425,712 - -0.2 2,425,724 - -0.2 2,425,749 - +0.4 2,425,812 + +0.2 2,425,833 - +0.1 2,425,989 + +1.9 2,426,067 - -0.1 2,426,119 + -0.4 2,426,137 - -0.7 2,426,248 + ABZR88_RS09725 0.23 -0.5 2,426,249 - ABZR88_RS09725 0.23 -0.2 2,426,320 - ABZR88_RS09725 0.38 -0.1 2,426,330 - ABZR88_RS09725 0.40 -1.0 2,426,330 - ABZR88_RS09725 0.40 -0.1 2,426,420 + ABZR88_RS09725 0.60 -1.1 2,426,570 + -0.1 2,426,594 + -0.1 2,426,651 - +0.5 2,426,686 - +0.0 2,426,686 - -0.5 2,426,729 - -0.1 2,426,751 - +0.2 2,426,751 - +0.0
Or see this region's nucleotide sequence