Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF692 and GFF693 are separated by 92 nucleotides GFF693 and GFF694 are separated by 9 nucleotides GFF694 and GFF695 are separated by 166 nucleotides GFF695 and GFF696 are separated by 17 nucleotides
GFF692 - Methionine--tRNA ligase, at 741,726 to 743,279
GFF692
GFF693 - hypothetical protein, at 743,372 to 743,881
GFF693
GFF694 - Biotin transporter BioY2, at 743,891 to 744,472
GFF694
GFF695 - D-aminoacyl-tRNA deacylase, at 744,639 to 745,415
GFF695
GFF696 - hypothetical protein, at 745,433 to 745,972
GFF696
Position (kb) 743
744
745 Strain fitness (log2 ratio) -1
0
1 at 743.320 kb on + strand at 743.330 kb on + strand at 743.468 kb on + strand, within GFF693 at 743.616 kb on - strand, within GFF693 at 743.730 kb on - strand, within GFF693 at 744.249 kb on + strand, within GFF694 at 744.312 kb on + strand, within GFF694 at 744.312 kb on + strand, within GFF694 at 744.312 kb on + strand, within GFF694 at 744.313 kb on - strand, within GFF694 at 744.313 kb on - strand, within GFF694 at 744.524 kb on + strand at 744.607 kb on + strand at 744.607 kb on + strand at 744.927 kb on + strand, within GFF695 at 744.927 kb on + strand, within GFF695 at 744.928 kb on - strand, within GFF695 at 744.928 kb on - strand, within GFF695 at 744.934 kb on - strand, within GFF695 at 744.934 kb on - strand, within GFF695 at 745.152 kb on + strand, within GFF695 at 745.152 kb on + strand, within GFF695 at 745.386 kb on + strand at 745.387 kb on - strand at 745.387 kb on - strand at 745.460 kb on + strand at 745.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 743,320 + +0.2 743,330 + -1.3 743,468 + GFF693 0.19 -0.3 743,616 - GFF693 0.48 +1.6 743,730 - GFF693 0.70 +0.7 744,249 + GFF694 0.62 +0.9 744,312 + GFF694 0.72 +0.1 744,312 + GFF694 0.72 +1.1 744,312 + GFF694 0.72 -0.1 744,313 - GFF694 0.73 +1.3 744,313 - GFF694 0.73 +0.7 744,524 + -0.6 744,607 + +0.5 744,607 + +0.2 744,927 + GFF695 0.37 +0.1 744,927 + GFF695 0.37 +0.0 744,928 - GFF695 0.37 +0.3 744,928 - GFF695 0.37 +0.1 744,934 - GFF695 0.38 -0.2 744,934 - GFF695 0.38 -0.6 745,152 + GFF695 0.66 -0.7 745,152 + GFF695 0.66 +0.8 745,386 + -0.9 745,387 - +0.8 745,387 - +0.1 745,460 + +0.3 745,463 - -0.0
Or see this region's nucleotide sequence