Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF677 and GFF678 are separated by 4 nucleotides GFF678 and GFF679 are separated by 17 nucleotides GFF679 and GFF680 overlap by 4 nucleotides GFF680 and GFF681 are separated by 176 nucleotides
GFF677 - Glutamine transport ATP-binding protein GlnQ, at 727,145 to 727,975
GFF677
GFF678 - putative 2-dehydro-3-deoxygalactonokinase DgoK1, at 727,980 to 728,885
GFF678
GFF679 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 728,903 to 729,532
GFF679
GFF680 - Putative L-lactate dehydrogenase operon regulatory protein, at 729,529 to 730,311
GFF680
GFF681 - L-lactate permease, at 730,488 to 732,194
GFF681
Position (kb)
728
729
730 Strain fitness (log2 ratio)
-1
0
1 at 728.031 kb on + strand at 728.031 kb on + strand at 728.032 kb on - strand at 728.032 kb on - strand at 728.220 kb on + strand, within GFF678 at 728.221 kb on - strand, within GFF678 at 728.221 kb on - strand, within GFF678 at 728.449 kb on + strand, within GFF678 at 728.481 kb on + strand, within GFF678 at 729.404 kb on + strand, within GFF679 at 729.710 kb on + strand, within GFF680 at 729.711 kb on - strand, within GFF680 at 729.711 kb on - strand, within GFF680 at 729.727 kb on - strand, within GFF680 at 729.727 kb on - strand, within GFF680 at 729.728 kb on + strand, within GFF680 at 729.728 kb on + strand, within GFF680 at 729.728 kb on + strand, within GFF680 at 729.728 kb on + strand, within GFF680 at 729.728 kb on + strand, within GFF680 at 729.729 kb on - strand, within GFF680 at 729.729 kb on - strand, within GFF680 at 729.729 kb on - strand, within GFF680 at 729.729 kb on - strand, within GFF680 at 729.729 kb on - strand, within GFF680 at 730.381 kb on + strand at 730.381 kb on + strand at 730.382 kb on - strand at 730.382 kb on - strand at 730.382 kb on - strand at 730.407 kb on - strand at 730.514 kb on - strand at 730.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 728,031 + -1.3 728,031 + -0.5 728,032 - +0.1 728,032 - +0.2 728,220 + GFF678 0.26 +0.3 728,221 - GFF678 0.27 -0.1 728,221 - GFF678 0.27 +0.8 728,449 + GFF678 0.52 +0.8 728,481 + GFF678 0.55 +0.3 729,404 + GFF679 0.80 +0.3 729,710 + GFF680 0.23 -0.6 729,711 - GFF680 0.23 -0.7 729,711 - GFF680 0.23 +0.4 729,727 - GFF680 0.25 +1.4 729,727 - GFF680 0.25 -0.4 729,728 + GFF680 0.25 -0.6 729,728 + GFF680 0.25 -0.3 729,728 + GFF680 0.25 -0.7 729,728 + GFF680 0.25 +0.6 729,728 + GFF680 0.25 -1.1 729,729 - GFF680 0.26 +0.1 729,729 - GFF680 0.26 +1.2 729,729 - GFF680 0.26 +0.5 729,729 - GFF680 0.26 +0.4 729,729 - GFF680 0.26 +0.7 730,381 + +0.1 730,381 + -1.2 730,382 - -0.5 730,382 - -0.1 730,382 - -1.0 730,407 - +0.0 730,514 - +1.0 730,527 - +0.4
Or see this region's nucleotide sequence