Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4254 and GFF4255 are separated by 42 nucleotides GFF4255 and GFF4256 are separated by 121 nucleotides GFF4256 and GFF4257 are separated by 127 nucleotides GFF4257 and GFF4258 are separated by 96 nucleotides
GFF4254 - Polyphosphate:AMP phosphotransferase, at 24,312 to 25,799
GFF4254
GFF4255 - (R)-specific enoyl-CoA hydratase, at 25,842 to 26,294
GFF4255
GFF4256 - Chaperone protein DnaJ, at 26,416 to 27,366
GFF4256
GFF4257 - hypothetical protein, at 27,494 to 27,901
GFF4257
GFF4258 - Pyridoxine/pyridoxamine 5'-phosphate oxidase, at 27,998 to 28,639
GFF4258
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 25.477 kb on + strand, within GFF4254 at 25.478 kb on - strand, within GFF4254 at 25.478 kb on - strand, within GFF4254 at 25.532 kb on - strand, within GFF4254 at 25.616 kb on - strand, within GFF4254 at 25.616 kb on - strand, within GFF4254 at 25.616 kb on - strand, within GFF4254 at 25.833 kb on + strand at 25.833 kb on + strand at 26.044 kb on + strand, within GFF4255 at 26.045 kb on - strand, within GFF4255 at 26.104 kb on + strand, within GFF4255 at 26.105 kb on - strand, within GFF4255 at 26.310 kb on - strand at 26.332 kb on + strand at 26.333 kb on - strand at 26.371 kb on + strand at 26.482 kb on + strand at 26.483 kb on - strand at 26.656 kb on + strand, within GFF4256 at 26.657 kb on - strand, within GFF4256 at 26.714 kb on + strand, within GFF4256 at 26.730 kb on - strand, within GFF4256 at 26.730 kb on - strand, within GFF4256 at 26.730 kb on - strand, within GFF4256 at 27.177 kb on - strand, within GFF4256 at 27.353 kb on + strand at 27.606 kb on + strand, within GFF4257 at 27.938 kb on - strand at 27.938 kb on - strand at 27.939 kb on + strand at 27.940 kb on - strand at 28.035 kb on + strand at 28.036 kb on - strand at 28.189 kb on + strand, within GFF4258 at 28.189 kb on + strand, within GFF4258 at 28.190 kb on - strand, within GFF4258 at 28.361 kb on + strand, within GFF4258
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 25,477 + GFF4254 0.78 +0.4 25,478 - GFF4254 0.78 +0.8 25,478 - GFF4254 0.78 +0.5 25,532 - GFF4254 0.82 +0.2 25,616 - GFF4254 0.88 +0.5 25,616 - GFF4254 0.88 -3.1 25,616 - GFF4254 0.88 +0.0 25,833 + -0.9 25,833 + -0.3 26,044 + GFF4255 0.45 -0.4 26,045 - GFF4255 0.45 +0.5 26,104 + GFF4255 0.58 -0.5 26,105 - GFF4255 0.58 -0.6 26,310 - +0.1 26,332 + +0.2 26,333 - -0.8 26,371 + +0.3 26,482 + +0.3 26,483 - -1.7 26,656 + GFF4256 0.25 +0.0 26,657 - GFF4256 0.25 -0.5 26,714 + GFF4256 0.31 -0.8 26,730 - GFF4256 0.33 -0.5 26,730 - GFF4256 0.33 -0.2 26,730 - GFF4256 0.33 +0.6 27,177 - GFF4256 0.80 -0.1 27,353 + -0.4 27,606 + GFF4257 0.27 +1.2 27,938 - -1.3 27,938 - -0.3 27,939 + -2.7 27,940 - -0.1 28,035 + +0.4 28,036 - -0.8 28,189 + GFF4258 0.30 +0.7 28,189 + GFF4258 0.30 -0.8 28,190 - GFF4258 0.30 -1.3 28,361 + GFF4258 0.57 -0.6
Or see this region's nucleotide sequence