Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4165 and GFF4166 are separated by 301 nucleotides GFF4166 and GFF4167 are separated by 36 nucleotides GFF4167 and GFF4168 are separated by 165 nucleotides
GFF4165 - NADP-dependent 3-hydroxy acid dehydrogenase YdfG, at 178,628 to 179,401
GFF4165
GFF4166 - hypothetical protein, at 179,703 to 180,263
GFF4166
GFF4167 - 5,6-dimethylbenzimidazole synthase, at 180,300 to 180,968
GFF4167
GFF4168 - Glycerol kinase, at 181,134 to 182,621
GFF4168
Position (kb) 180
181 Strain fitness (log2 ratio) -1
0
1 at 179.432 kb on - strand at 179.673 kb on + strand at 180.301 kb on + strand at 180.302 kb on - strand at 180.565 kb on + strand, within GFF4167 at 180.566 kb on - strand, within GFF4167 at 180.566 kb on - strand, within GFF4167 at 180.566 kb on - strand, within GFF4167 at 180.567 kb on + strand, within GFF4167 at 180.600 kb on + strand, within GFF4167 at 180.922 kb on + strand at 180.923 kb on - strand at 180.993 kb on + strand at 181.294 kb on + strand, within GFF4168 at 181.708 kb on + strand, within GFF4168 at 181.729 kb on + strand, within GFF4168 at 181.729 kb on + strand, within GFF4168 at 181.729 kb on + strand, within GFF4168 at 181.730 kb on - strand, within GFF4168 at 181.730 kb on - strand, within GFF4168 at 181.730 kb on - strand, within GFF4168 at 181.754 kb on - strand, within GFF4168 at 181.834 kb on + strand, within GFF4168
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 179,432 - +0.4 179,673 + -0.3 180,301 + -0.5 180,302 - +0.1 180,565 + GFF4167 0.40 +0.0 180,566 - GFF4167 0.40 +1.5 180,566 - GFF4167 0.40 +0.2 180,566 - GFF4167 0.40 +0.3 180,567 + GFF4167 0.40 -0.8 180,600 + GFF4167 0.45 +0.3 180,922 + +0.4 180,923 - -0.6 180,993 + +0.7 181,294 + GFF4168 0.11 +0.2 181,708 + GFF4168 0.39 -0.4 181,729 + GFF4168 0.40 -0.0 181,729 + GFF4168 0.40 +0.4 181,729 + GFF4168 0.40 +0.5 181,730 - GFF4168 0.40 -0.3 181,730 - GFF4168 0.40 +0.4 181,730 - GFF4168 0.40 -0.8 181,754 - GFF4168 0.42 -0.2 181,834 + GFF4168 0.47 +0.1
Or see this region's nucleotide sequence