Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4065 and GFF4066 are separated by 14 nucleotides GFF4066 and GFF4067 are separated by 9 nucleotides GFF4067 and GFF4068 are separated by 2 nucleotides GFF4068 and GFF4069 overlap by 4 nucleotides GFF4069 and GFF4070 overlap by 4 nucleotides
GFF4065 - L(+)-tartrate dehydratase subunit beta, at 59,728 to 60,339
GFF4065
GFF4066 - L(+)-tartrate dehydratase subunit alpha, at 60,354 to 61,226
GFF4066
GFF4067 - hypothetical protein, at 61,236 to 61,967
GFF4067
GFF4068 - hypothetical protein, at 61,970 to 62,350
GFF4068
GFF4069 - hypothetical protein, at 62,347 to 62,691
GFF4069
GFF4070 - Fumarate reductase iron-sulfur subunit, at 62,688 to 63,464
GFF4070
Position (kb) 61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.465 kb on + strand, within GFF4066 at 60.465 kb on + strand, within GFF4066 at 60.466 kb on - strand, within GFF4066 at 60.466 kb on - strand, within GFF4066 at 60.921 kb on - strand, within GFF4066 at 61.009 kb on + strand, within GFF4066 at 61.009 kb on + strand, within GFF4066 at 61.009 kb on + strand, within GFF4066 at 61.009 kb on + strand, within GFF4066 at 61.009 kb on + strand, within GFF4066 at 61.180 kb on + strand at 61.180 kb on + strand at 61.181 kb on - strand at 61.237 kb on + strand at 61.238 kb on - strand at 61.238 kb on - strand at 61.315 kb on + strand, within GFF4067 at 61.453 kb on + strand, within GFF4067 at 61.453 kb on + strand, within GFF4067 at 61.454 kb on - strand, within GFF4067 at 61.454 kb on - strand, within GFF4067 at 61.633 kb on + strand, within GFF4067 at 61.633 kb on + strand, within GFF4067 at 61.633 kb on + strand, within GFF4067 at 61.633 kb on + strand, within GFF4067 at 61.633 kb on + strand, within GFF4067 at 61.634 kb on - strand, within GFF4067 at 61.634 kb on - strand, within GFF4067 at 61.634 kb on - strand, within GFF4067 at 61.634 kb on - strand, within GFF4067 at 61.634 kb on - strand, within GFF4067 at 62.372 kb on + strand at 62.505 kb on + strand, within GFF4069 at 62.531 kb on + strand, within GFF4069 at 62.531 kb on + strand, within GFF4069 at 62.675 kb on + strand at 62.675 kb on + strand at 62.675 kb on + strand at 62.675 kb on + strand at 62.675 kb on + strand at 62.675 kb on + strand at 62.675 kb on + strand at 62.676 kb on - strand at 62.764 kb on + strand at 62.764 kb on + strand at 62.765 kb on - strand at 62.929 kb on + strand, within GFF4070 at 62.929 kb on + strand, within GFF4070 at 62.930 kb on - strand, within GFF4070 at 62.930 kb on - strand, within GFF4070 at 62.966 kb on - strand, within GFF4070
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 60,465 + GFF4066 0.13 -1.8 60,465 + GFF4066 0.13 -0.3 60,466 - GFF4066 0.13 -1.1 60,466 - GFF4066 0.13 +1.8 60,921 - GFF4066 0.65 +0.9 61,009 + GFF4066 0.75 -1.7 61,009 + GFF4066 0.75 -0.4 61,009 + GFF4066 0.75 -0.1 61,009 + GFF4066 0.75 +1.2 61,009 + GFF4066 0.75 +0.4 61,180 + +0.4 61,180 + -0.1 61,181 - +0.1 61,237 + -1.1 61,238 - -0.3 61,238 - +0.6 61,315 + GFF4067 0.11 -0.1 61,453 + GFF4067 0.30 -0.6 61,453 + GFF4067 0.30 +0.8 61,454 - GFF4067 0.30 -0.8 61,454 - GFF4067 0.30 +0.1 61,633 + GFF4067 0.54 +0.1 61,633 + GFF4067 0.54 -0.4 61,633 + GFF4067 0.54 +0.4 61,633 + GFF4067 0.54 -0.3 61,633 + GFF4067 0.54 -0.2 61,634 - GFF4067 0.54 -0.0 61,634 - GFF4067 0.54 -0.0 61,634 - GFF4067 0.54 +0.6 61,634 - GFF4067 0.54 -0.1 61,634 - GFF4067 0.54 -0.1 62,372 + +1.5 62,505 + GFF4069 0.46 -0.4 62,531 + GFF4069 0.53 +0.1 62,531 + GFF4069 0.53 -1.2 62,675 + +0.3 62,675 + -0.7 62,675 + -0.4 62,675 + +0.3 62,675 + -0.4 62,675 + -1.3 62,675 + +1.0 62,676 - -0.3 62,764 + -0.8 62,764 + +0.3 62,765 - -0.8 62,929 + GFF4070 0.31 -0.2 62,929 + GFF4070 0.31 -0.2 62,930 - GFF4070 0.31 -0.2 62,930 - GFF4070 0.31 -0.7 62,966 - GFF4070 0.36 -0.1
Or see this region's nucleotide sequence