Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3890 and GFF3891 are separated by 5 nucleotides GFF3891 and GFF3892 are separated by 171 nucleotides GFF3892 and GFF3893 are separated by 117 nucleotides GFF3893 and GFF3894 are separated by 199 nucleotides
GFF3890 - Trehalose-6-phosphate synthase, at 257,563 to 259,038
GFF3890
GFF3891 - Trehalose-6-phosphate phosphatase, at 259,044 to 259,835
GFF3891
GFF3892 - hypothetical protein, at 260,007 to 260,402
GFF3892
GFF3893 - hypothetical protein, at 260,520 to 260,789
GFF3893
GFF3894 - hypothetical protein, at 260,989 to 261,165
GFF3894
Position (kb) 260
261 Strain fitness (log2 ratio) -1
0
1
2 at 259.554 kb on + strand, within GFF3891 at 259.866 kb on - strand at 260.006 kb on + strand at 260.006 kb on + strand at 260.006 kb on + strand at 260.006 kb on + strand at 260.007 kb on - strand at 260.007 kb on - strand at 260.204 kb on + strand, within GFF3892 at 260.205 kb on - strand, within GFF3892 at 260.205 kb on - strand, within GFF3892 at 260.314 kb on + strand, within GFF3892 at 260.315 kb on - strand, within GFF3892 at 260.315 kb on - strand, within GFF3892 at 260.317 kb on - strand, within GFF3892 at 260.317 kb on - strand, within GFF3892 at 260.317 kb on - strand, within GFF3892 at 260.324 kb on - strand, within GFF3892 at 260.400 kb on + strand at 260.510 kb on + strand at 260.892 kb on + strand at 260.906 kb on - strand at 261.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 259,554 + GFF3891 0.64 +0.7 259,866 - +0.3 260,006 + +0.8 260,006 + -0.5 260,006 + +0.6 260,006 + -1.0 260,007 - -0.4 260,007 - +0.3 260,204 + GFF3892 0.50 +1.8 260,205 - GFF3892 0.50 +0.4 260,205 - GFF3892 0.50 +1.1 260,314 + GFF3892 0.78 +0.5 260,315 - GFF3892 0.78 +2.0 260,315 - GFF3892 0.78 +0.9 260,317 - GFF3892 0.78 +0.5 260,317 - GFF3892 0.78 +0.3 260,317 - GFF3892 0.78 +0.4 260,324 - GFF3892 0.80 -0.2 260,400 + +0.1 260,510 + +0.5 260,892 + +0.6 260,906 - -0.9 261,172 - +0.3
Or see this region's nucleotide sequence