Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3889 and GFF3890 are separated by 290 nucleotides GFF3890 and GFF3891 are separated by 5 nucleotides GFF3891 and GFF3892 are separated by 171 nucleotides
GFF3889 - hypothetical protein, at 256,748 to 257,272
GFF3889
GFF3890 - Trehalose-6-phosphate synthase, at 257,563 to 259,038
GFF3890
GFF3891 - Trehalose-6-phosphate phosphatase, at 259,044 to 259,835
GFF3891
GFF3892 - hypothetical protein, at 260,007 to 260,402
GFF3892
Position (kb) 257
258
259
260 Strain fitness (log2 ratio) -2
-1
0
1
2 at 256.826 kb on + strand, within GFF3889 at 256.827 kb on - strand, within GFF3889 at 256.828 kb on + strand, within GFF3889 at 256.829 kb on - strand, within GFF3889 at 256.829 kb on - strand, within GFF3889 at 256.836 kb on - strand, within GFF3889 at 257.009 kb on + strand, within GFF3889 at 257.010 kb on - strand, within GFF3889 at 257.010 kb on - strand, within GFF3889 at 257.099 kb on - strand, within GFF3889 at 257.501 kb on - strand at 257.501 kb on - strand at 257.503 kb on - strand at 257.503 kb on - strand at 257.542 kb on + strand at 257.542 kb on + strand at 257.543 kb on - strand at 257.559 kb on - strand at 257.560 kb on + strand at 257.560 kb on + strand at 257.988 kb on - strand, within GFF3890 at 258.060 kb on - strand, within GFF3890 at 258.060 kb on - strand, within GFF3890 at 258.497 kb on + strand, within GFF3890 at 258.617 kb on + strand, within GFF3890 at 258.741 kb on - strand, within GFF3890 at 258.809 kb on + strand, within GFF3890 at 258.849 kb on - strand, within GFF3890 at 259.554 kb on + strand, within GFF3891 at 259.866 kb on - strand at 260.006 kb on + strand at 260.006 kb on + strand at 260.006 kb on + strand at 260.006 kb on + strand at 260.007 kb on - strand at 260.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 256,826 + GFF3889 0.15 +0.1 256,827 - GFF3889 0.15 -1.3 256,828 + GFF3889 0.15 +0.4 256,829 - GFF3889 0.15 +0.1 256,829 - GFF3889 0.15 -0.8 256,836 - GFF3889 0.17 -0.2 257,009 + GFF3889 0.50 +2.0 257,010 - GFF3889 0.50 +0.4 257,010 - GFF3889 0.50 +0.6 257,099 - GFF3889 0.67 -0.4 257,501 - -0.3 257,501 - +0.3 257,503 - -0.1 257,503 - +0.6 257,542 + +1.2 257,542 + +1.2 257,543 - +0.4 257,559 - -2.7 257,560 + +1.1 257,560 + +0.9 257,988 - GFF3890 0.29 +2.1 258,060 - GFF3890 0.34 +0.4 258,060 - GFF3890 0.34 +0.2 258,497 + GFF3890 0.63 +0.2 258,617 + GFF3890 0.71 +0.7 258,741 - GFF3890 0.80 -1.6 258,809 + GFF3890 0.84 -1.1 258,849 - GFF3890 0.87 +0.5 259,554 + GFF3891 0.64 +0.7 259,866 - +0.3 260,006 + +0.8 260,006 + -0.5 260,006 + +0.6 260,006 + -1.0 260,007 - -0.4 260,007 - +0.3
Or see this region's nucleotide sequence