Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3853 and GFF3854 are separated by 119 nucleotides GFF3854 and GFF3855 are separated by 533 nucleotides GFF3855 and GFF3856 are separated by 206 nucleotides
GFF3853 - Protease HtpX, at 221,131 to 222,042
GFF3853
GFF3854 - hypothetical protein, at 222,162 to 222,368
GFF3854
GFF3855 - 5,6,7,8-tetrahydromethanopterin hydro-lyase, at 222,902 to 223,408
GFF3855
GFF3856 - Hydrazine synthase subunit beta, at 223,615 to 224,595
GFF3856
Position (kb) 222
223
224 Strain fitness (log2 ratio) -1
0
1 at 222.085 kb on - strand at 222.085 kb on - strand at 222.352 kb on + strand at 222.353 kb on - strand at 222.556 kb on + strand at 222.573 kb on - strand at 222.826 kb on - strand at 222.834 kb on + strand at 222.835 kb on - strand at 222.875 kb on + strand at 222.907 kb on + strand at 222.908 kb on - strand at 222.913 kb on + strand at 222.914 kb on - strand at 223.166 kb on + strand, within GFF3855 at 223.298 kb on + strand, within GFF3855 at 223.299 kb on - strand, within GFF3855 at 223.305 kb on - strand, within GFF3855 at 223.407 kb on - strand at 223.494 kb on - strand at 223.582 kb on + strand at 223.739 kb on + strand, within GFF3856 at 223.739 kb on + strand, within GFF3856 at 223.740 kb on - strand, within GFF3856 at 223.793 kb on + strand, within GFF3856 at 223.850 kb on + strand, within GFF3856 at 223.896 kb on - strand, within GFF3856 at 223.983 kb on + strand, within GFF3856 at 223.983 kb on + strand, within GFF3856 at 223.986 kb on - strand, within GFF3856 at 223.986 kb on - strand, within GFF3856 at 224.081 kb on + strand, within GFF3856 at 224.222 kb on + strand, within GFF3856 at 224.222 kb on + strand, within GFF3856 at 224.223 kb on - strand, within GFF3856 at 224.237 kb on + strand, within GFF3856 at 224.238 kb on - strand, within GFF3856 at 224.238 kb on - strand, within GFF3856
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 222,085 - +0.5 222,085 - +0.3 222,352 + +0.5 222,353 - +0.2 222,556 + +0.8 222,573 - +0.2 222,826 - -0.6 222,834 + -0.5 222,835 - +0.1 222,875 + -1.0 222,907 + +0.3 222,908 - +1.1 222,913 + +0.5 222,914 - +0.3 223,166 + GFF3855 0.52 +0.4 223,298 + GFF3855 0.78 -1.6 223,299 - GFF3855 0.78 -0.6 223,305 - GFF3855 0.79 -0.0 223,407 - +0.7 223,494 - -0.5 223,582 + -0.1 223,739 + GFF3856 0.13 -0.5 223,739 + GFF3856 0.13 +0.5 223,740 - GFF3856 0.13 -0.3 223,793 + GFF3856 0.18 +0.7 223,850 + GFF3856 0.24 +0.2 223,896 - GFF3856 0.29 -1.1 223,983 + GFF3856 0.38 +0.5 223,983 + GFF3856 0.38 +0.3 223,986 - GFF3856 0.38 +0.1 223,986 - GFF3856 0.38 +0.4 224,081 + GFF3856 0.48 -0.5 224,222 + GFF3856 0.62 -0.5 224,222 + GFF3856 0.62 -0.2 224,223 - GFF3856 0.62 -0.2 224,237 + GFF3856 0.63 +0.1 224,238 - GFF3856 0.64 -1.2 224,238 - GFF3856 0.64 -0.1
Or see this region's nucleotide sequence