Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF376 and GFF377 overlap by 4 nucleotides GFF377 and GFF378 overlap by 15 nucleotides GFF378 and GFF379 are separated by 182 nucleotides
GFF376 - hypothetical protein, at 389,351 to 390,523
GFF376
GFF377 - hypothetical protein, at 390,520 to 391,104
GFF377
GFF378 - hypothetical protein, at 391,090 to 392,097
GFF378
GFF379 - Thiol:disulfide interchange protein DsbD, at 392,280 to 393,011
GFF379
Position (kb) 391
392
393 Strain fitness (log2 ratio) -1
0
1 at 390.254 kb on + strand, within GFF376 at 390.377 kb on + strand, within GFF376 at 390.378 kb on - strand, within GFF376 at 390.378 kb on - strand, within GFF376 at 390.427 kb on + strand at 390.428 kb on - strand at 390.428 kb on - strand at 391.064 kb on - strand at 391.064 kb on - strand at 391.091 kb on + strand at 391.091 kb on + strand at 391.092 kb on - strand at 391.092 kb on - strand at 391.317 kb on + strand, within GFF378 at 391.409 kb on + strand, within GFF378 at 391.409 kb on + strand, within GFF378 at 391.410 kb on - strand, within GFF378 at 391.415 kb on + strand, within GFF378 at 391.868 kb on + strand, within GFF378 at 391.869 kb on - strand, within GFF378 at 391.869 kb on - strand, within GFF378 at 391.902 kb on - strand, within GFF378 at 392.113 kb on + strand at 393.042 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 390,254 + GFF376 0.77 -0.3 390,377 + GFF376 0.87 -0.5 390,378 - GFF376 0.88 +0.0 390,378 - GFF376 0.88 -0.1 390,427 + -0.7 390,428 - +0.7 390,428 - -1.6 391,064 - -0.2 391,064 - -0.6 391,091 + +0.4 391,091 + -1.3 391,092 - +0.8 391,092 - -0.0 391,317 + GFF378 0.23 +0.4 391,409 + GFF378 0.32 -0.6 391,409 + GFF378 0.32 +0.3 391,410 - GFF378 0.32 -0.1 391,415 + GFF378 0.32 +0.6 391,868 + GFF378 0.77 -0.3 391,869 - GFF378 0.77 -1.6 391,869 - GFF378 0.77 -1.0 391,902 - GFF378 0.81 +0.3 392,113 + +0.7 393,042 + -0.6
Or see this region's nucleotide sequence